Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Food-borne pathogen numerator detection mark establishing method

A food-borne pathogenic bacteria and molecular detection technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc. High, false detection methods, etc., to achieve the effect of large number and saving financial resources

Inactive Publication Date: 2009-01-14
SHANGHAI UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for establishing molecular detection markers of food-borne pathogens by overcoming the problems of low specificity of food-borne pathogenic bacteria molecular detection markers and false detection in detection methods in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Food-borne pathogen numerator detection mark establishing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The concrete steps of embodiment one are as follows:

[0021] (1) Collection of gene sequences of Staphylococcus aureus and comparison strains; from GenBank (ftp: / / ftp.ncbi.nih.gov / genbank / genomes / Bacteria / ) all 14 genome sequences of Staphylococcus aureus that have been published ( Staphylococcus aureus NCTC 8325, USA300, RF122, COL, Mu50, N315, MRSA252, JH1, JH9, MSSA476 and MW2) and all other microbial genome sequences that have been sequenced.

[0022] (2) Analyze and obtain the conserved sequence fragments of Staphylococcus aureus: Segment the Staphylococcus aureus ATCC N315 genome according to the length of 500bp, compare and analyze each fragment with the genomes of other Staphylococcus aureus one by one using BLASTN, and retain Sequence fragments with a similarity of more than 98%.

[0023] (3) Acquisition of nucleic acid sequence fragments specific to food-borne pathogenic bacteria species: compare and analyze any fragment of the conserved nucleic acid fragmen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a building method for a food borne pathogen molecule detecting marker. The method adopts the following steps: all genome sequences of a food borne pathogen are collected; one genome selected from the genome sequences is divided into segments with a length of 500 bp to 1000 bp, each divided segment is compared with other genome sequences of the food borne pathogen, the divided segments with the similarity above 98 percent are retained, and conservative sequence segments of the food borne pathogen can be obtained; when one segment in the conservative sequence segments is compared with other microbial genome sequences, segments in conservative sequence segments which are identical with other microbial genome sequences are eliminated, so the retained sequence segments serve as a specific marker group for the food borne pathogen; and sieving is performed on the data information in the specific marker group for obtaining the molecule detecting marker of the food borne pathogen. The invention provides the molecule detecting and labeling method by using the combination of Bioinformatics and microbial Genomics for exploring the food borne pathogen for the first time, the obtained detecting marker has a higher specificity in comparison with the existing molecule detecting marker, and experiments of molecular biology such as molecular cloning, gene sequence testing and PCR are not required to be conducted, so the financial resources are saved.

Description

technical field [0001] The invention relates to a method for establishing molecular detection markers of food-borne pathogenic bacteria. technical background [0002] Foodborne pathogens are important microorganisms that cause food poisoning and endanger human health. They are the most important cause of food safety in both developed and developing countries. Among them, Staphylococcus aureus, Listeria monocytogenes, Salmonella Bacillus parahaemolyticus and Vibrio parahaemolyticus are internationally recognized as the four major pathogenic microorganisms with a wide range of hazards. In recent years, there have been outbreaks of foodborne diseases caused by the above-mentioned pathogenic bacteria in many countries around the world. Staphylococcus aureus (Staphylococcus aureus) is an important pathogen that causes human infection and bacterial food poisoning, and the frequency of food poisoning caused by it is extremely high. In my country, the poisoning cases caused by Sta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
CPCY02A50/30
Inventor 刘战民尹京苑
Owner SHANGHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com