Method and reagent kit for analyzing and diagnosing bladder cancer by means of uropsammus DNA methylation profile

A methylation profile, bladder cancer technology, applied in the field of bladder cancer diagnosis, can solve problems such as high levels in urine

Active Publication Date: 2009-01-28
SHANGHAI INST OF ONCOLOGY
View PDF0 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the quantification of MNP22 protein in urine is more sensitive than urine sediment cytology, there are also high levels in the urine of patients with benign urogenital diseases (hemuria, cystitis, kidney stones or urinary tract infection) Seriously insufficient [24]

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and reagent kit for analyzing and diagnosing bladder cancer by means of uropsammus DNA methylation profile
  • Method and reagent kit for analyzing and diagnosing bladder cancer by means of uropsammus DNA methylation profile
  • Method and reagent kit for analyzing and diagnosing bladder cancer by means of uropsammus DNA methylation profile

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] In one aspect, the invention provides a method of detecting whether a subject has bladder cancer, comprising the steps of:

[0024] 1. Provide a sample of the subject's urine sediment.

[0025] 2. A method of determining the pattern of methylation of one or more genes in a urine sediment. The genes are: ABCC13, ABCC6, ABCC8, ALX4, APC, BCAR3, BCL2, BMP3B, BNIP3, BRCA1, BRCA2, CBR1, CBR3, CCNA1, CDH1, CDH13, CDKN1C, CFTR, COX2, DAPK1, DRG1, DRM, EDNRB , FADD, GALC, GSTP1, HNF3B, HPP1, HTERT, ICAM1, ITGA4, LAMA3, LITAF, MAGEA1, MDR1, MGMT, MINT1, MINT2, MT1GMT, MINT1, MINT2, MT1A, MTSS1, MYOD1, OCLN, p14ARF, p16INK4a, PTCHD2 , RASSF1A, RPRM, RUNX3, SALL3, SERPINB5, SLC29A1, STAT1, TIMP3, TMS1, TNFRSF10A, TNFRSF10C, TNFRSF10D, TNFRSF21 and WWOX.

[0026] 3. Comparing the methylation profiles of the genes in the urine sediment samples of the subject and the normal subject, if there is one or more genes in a hypermethylated state, it means that the subject has bladder canc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method used for detecting whether a detected object suffers from bladder cancer, comprising the following steps: (a) the detected object provides a urinary sediment sample; (b) the methylation spectrum formula of a specific sequence (named as a gene in the following) in one or a plurality of gene promoter areas CpG in the sample is measured; and (c) the methylation spectrum formula of the detected object is compared with that of a normal object, if one or a plurality of genes are in high methylation state, the detected object is proved to suffer from the bladder cancer. The invention also provides a set of kit used for diagnosing the bladder cancer.

Description

technical field [0001] The present invention relates to the diagnosis of bladder cancer by detecting the difference in the methylation pattern of a specific sequence in the CpG island of the promoter region of the gene in the urine sediment from normal (or non-bladder cancer) and bladder cancer (including precancerous stage) individuals. Kits and methods for cancer. Background technique [0002] The complete genome sequencing of human beings and more and more model organisms has enabled the elucidation of gene composition and function in development and disease processes to enter a new level of analysis and interpretation based on non-DNA sequence-based epigenetic information. The age of the center. Epigenetics consists of DNA methylation (cytosine [CpG] methylation), noncoding RNA, histone modifications, and chromatin remodeling. This information interface is between the stored genetic blueprint genomic DNA sequence and the phenotype determined by the expression pattern. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/154G01N33/57407C12Q1/6886G01N2800/60
Inventor 朱景德
Owner SHANGHAI INST OF ONCOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap