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Method for appraising royal jelly quality

An evaluation method and technology for royal jelly, which are applied in material inspection products, test food, measuring devices, etc., can solve the problems of difficult evaluation, uneven quality of royal jelly, lack of evaluation methods, etc., and achieve the effect of simple analysis method and high sensitivity

Inactive Publication Date: 2009-02-11
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of corresponding evaluation methods, quality supervision departments and processing and trading companies have so far been unable to effectively supervise and control the production and circulation of royal jelly, and it is difficult to make a true evaluation of the storage conditions and quality experienced by royal jelly, resulting in The quality of royal jelly varies in the market

Method used

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  • Method for appraising royal jelly quality
  • Method for appraising royal jelly quality
  • Method for appraising royal jelly quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Commercially available 5-hydroxymethyl-2-furfural (Sigma Company) was dissolved in methanol to prepare a standard solution with a concentration of 0.5 mg / kg.

[0026] Weigh 1.0 g of newly harvested Camellia royal jelly (Zhejiang), Vitex royal jelly (Beijing), Acacia royal jelly (Shandong), and Rapeseed royal jelly (Qinghai) into 10 mL plastic centrifuge tubes with lids, add 5 mL of deionized water, and vortex Shake for 5 minutes, ultrasonically extract for 3 minutes, centrifuge at 12,000 rpm for 10 minutes, and collect the supernatant; add 3 mL of deionized water to the residue, repeat the above extraction and centrifugation steps, discard the residue, and combine the supernatant twice. Add 1mL of 300g / L potassium ferrocyanide solution to the supernatant, mix well, then add 1mL of 150g / L zinc acetate solution, mix well, stand still for 30min and centrifuge at 10000rpm for 10min, take 5mL of supernatant to a 10mL volumetric flask In, make up to the mark with deionized wa...

Embodiment 2

[0029] Commercially available 5-hydroxymethyl-2-furfural (Sigma Company) was dissolved in methanol to prepare standard solutions with a concentration of 0.5 mg / kg.

[0030] Weigh 1.0 g of camellia royal jelly (Zhejiang), Vitex royal jelly (Beijing), Acacia royal jelly (Shandong), and rapeseed royal jelly (Qinghai) samples into 10 mL plastic centrifuge tubes with lids, add 5 mL of deionized water, vortex for 5 min, and ultrasonically After extraction for 3 minutes, centrifuge at 12,000 rpm for 10 minutes to collect the supernatant; add 3 mL of deionized water to the residue, repeat the extraction and centrifugation steps above, discard the residue, and combine the supernatant twice. Add 1mL of 300g / L potassium ferrocyanide solution to the supernatant, mix well, then add 1mL of 150g / L zinc acetate solution, mix well, stand still for 30min, then centrifuge at 10000rpm for 10min, take 5mL of the supernatant to a 10mL volumetric flask In, make up to the mark with deionized water. ...

Embodiment 3

[0033] Commercially available 5-hydroxymethyl-2-furfural (Sigma Company) was dissolved in methanol to prepare a standard solution with a concentration of 0.5 mg / kg.

[0034] Weigh commercially available royal jelly samples: A (Shandong, batch number 20070815), B (Beijing, batch number 20080409), C (Hubei, batch number 20070106) and D (Zhejiang, batch number 20071013) 1.0g in a 10mL plastic centrifuge tube with a cover, Add 5 mL of deionized water, vortex for 5 min, ultrasonically extract for 3 min, centrifuge at 12,000 rpm for 10 min, and collect the supernatant; add 3 mL of deionized water to the residue, repeat the above extraction and centrifugation steps, discard the residue, and combine twice supernatant. Add 1mL of 300g / L potassium ferrocyanide solution to the supernatant, mix well, then add 1mL of 150g / L zinc acetate solution, mix well, stand still for 30min and centrifuge at 10000rpm for 10min, take 5mL of supernatant to a 10mL volumetric flask In, make up to the mark...

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Abstract

The invention provides an evaluation method for the quality of royal jelly. The quality of royal jelly is judged by detecting the content of a furfurol substance in the royal jelly, namely, 5- hydroxymethyl-2-furfurol. The invention has the advantages that the sensitivity is high, the analysis method is simple, six furfurol substances namely, 5-hydroxymethyl-2-furfurol, 5-methyl hydroxymethyl, 2-hydroxymethyl, 2-furoic acid, 3-furoic acid, 5-hydroxymethyl-2-furoic acid can be effectively, sensitively and accurately separated through the method for detecting the furfurol substances, and accurate quantification for 5-hydroxymethyl-2-furfurol in the royal jelly can be realized.

Description

technical field [0001] The invention relates to the field of food quality evaluation, in particular to a method for evaluating the quality of royal jelly by measuring the content of furfural 5-hydroxymethyl-2-furfural in royal jelly. Background technique [0002] Royal jelly is jointly secreted by the hypopharyngeal gland (Hypopharyngealgland) and the mandibular gland (Mandibular gland) of the head of the nursing bee. things. Royal jelly plays an important role in the hierarchical differentiation of bees. The small larvae hatched from the fertilized eggs laid by the queen bee can develop into complete reproductive organs in only 16 days if they are fed with royal jelly throughout the larval stage. Queen bee; if only feeding royal jelly in the first three days of the larval stage, and then feeding honey and pollen, it takes 21 days and can only develop into worker bees with incomplete reproductive organs. [0003] The composition of royal jelly is very complex, rich in prot...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N30/02G01N21/25
Inventor 吴黎明胡福良薛晓锋赵静李熠周金慧
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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