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Production process for high purity porcine blood albumin and uses thereof

A production process, albumin technology, applied in the direction of serum albumin, albumin peptide, animal/human protein, etc., can solve the problems of not containing albumin and serum, complicated purification process, high cost, etc., and achieve low cost and excellent preparation process Simple, low-material effect

Active Publication Date: 2012-01-04
GUANGZHOU BIOSEAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these recombinant human albumin purification method patents still have some similar problems: the purification process is too complicated, the cycle is long, and the cost is high
However, the extraction process of this method only includes the process of removing globulin from bovine serum, and does not include the process step of separately separating albumin from serum, and the product may contain other components

Method used

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  • Production process for high purity porcine blood albumin and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take 56,000ml of fresh pig blood from pigs that have been strictly quarantined and free from infectious diseases, add it to 6,220ml of 3.8% sodium citrate and 0.9% sodium chloride solution, and let the blood stand in a 3°C freezer for 10 hours. The erythrocytes are stratified, and the plasma is absorbed by the siphon method, and the lower layer of blood cells is used separately; the above-mentioned separated plasma is placed in a centrifugal system with a refrigeration device, and the temperature of the refrigeration system is set at 5°C. Turn on the centrifuge and centrifuge at a speed of 5000 rpm minutes, centrifuged for 50 minutes, and collected plasma. The plasma is then filtered with a 0.22μm filter to obtain about 28,000ml of purified plasma;

[0038] Put the refined plasma into the interlayer reaction tank, and heat the interlayer tank to 70°C; take 93.1g of sodium octanoate and 252g of sodium chloride, add them to 28000ml of purified water and dissolve them, and...

Embodiment 2

[0040] Take 40,000ml of fresh pig blood from pigs without infectious diseases after strict quarantine, add it to 4450ml solution containing 3.8% sodium citrate and 0.9% sodium chloride, and put the blood in a cold storage at 8°C for 8 hours, then the plasma and The erythrocytes are stratified, and the plasma is absorbed by the siphon method, and the blood cells in the lower layer are used separately; the above-mentioned separated plasma is placed in a centrifugal system with a refrigeration device, and the temperature of the refrigeration system is set at 8°C. Turn on the centrifuge and centrifuge at a speed of 5000 rpm minutes, centrifuged for 45 minutes, and collected plasma. The plasma is then filtered with a 0.22μm filter to obtain about 20,000ml of purified plasma;

[0041] Put the refined plasma into the interlayer reaction tank, and heat the interlayer tank to 70°C; take 66.5g of sodium octanoate and 180g of sodium chloride, and add them to 20,000ml of purified water to...

Embodiment 3

[0043] Take 50,000ml of fresh pig blood from pigs without infectious diseases after strict quarantine, add it to 5560ml solution containing 3.8% sodium citrate and 0.9% sodium chloride, and put the blood in a cold storage at 5°C for 8 hours. The erythrocytes are stratified, and the plasma is absorbed by the siphon method, and the lower layer of blood cells is used separately; the above-mentioned separated plasma is placed in a centrifugal system with a refrigeration device, and the temperature of the refrigeration system is set to 10°C. Turn on the centrifuge and centrifuge at a speed of 3000 rpm minutes, centrifuged for 60 minutes, and collected plasma. The plasma is then filtered with a 0.22μm filter to obtain about 25,000ml of purified plasma;

[0044] Put the refined plasma into the interlayer reaction tank, and heat the interlayer tank to 70°C; take 83.2g of sodium octanoate and 225.0g of sodium chloride, add them to 25000ml of purified water and dissolve them, and add th...

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Abstract

The present invention discloses a technique for extracting high-purity porcine serum albumin. The technique includes the following steps: sodium citrate and sodium chloride are added into porcine blood to separate plasma and erythrocytes; after being centrifugated, filtered by a membrane and extracted, the plasma is put into an interlayered reactor; alcohol, sodium caprylate and sodium chloride are added into the plasma and stirred slowly after the interlayered reactor is heated; hydrochloric acid is added to adjust the pH value for two times; after centrifugation, supernatant is added into precipitating reagent, ground into paste and stirred; after being refrigerated overnight, the mixed solution is centrifugated and left for precipitation; the precipitate is resolved in water and, afterthe pH is adjusted by sodium hydroxide solution, kept still; supernatant is separated out by centrifugation; and after membrane filtration and virus inactivation, the filtrate is frozen out into the finished product. The technique has the advantages of little material usage, short process flow, high production efficiency, easy operation, low production cost and high product quality and is suitable for mass production. The product can be used as an auxiliary material for the preparation of fibrin glue sealant, a biochemical material and a substitute for other albumins.

Description

technical field [0001] The invention relates to a process for extracting high-purity albumin from pig serum and the use of pig serum albumin; specifically, it is a process for refining pig serum albumin by alcohol extraction and compound precipitation, and the obtained high-purity albumin is used as fibrin glue for sealing It can also be used as an auxiliary material for the preparation of pharmaceuticals, and can also be used as a biochemical raw material or a substitute for other albumin, belonging to the field of biotechnology. Background technique [0002] Serum albumin is a protein that performs a variety of important biological functions in blood circulation, detoxification, and lipid metabolism. It is widely used as a stabilizer of biological macromolecules (enzymes, antigens), model antigens and carriers of haptens in biochemistry and immunology research. Since albumin is a kind of protein, it is easily denatured by temperature, organic solvent and pH, etc. It is ve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/30C07K1/14C07K14/765
Inventor 黄星李吉来江彩霞肖尚志潘小宁
Owner GUANGZHOU BIOSEAL BIOTECH