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Seed selection method of cabbage type rape twofold sterile line

A cabbage type rapeseed double technology is applied in the field of crop heterosis utilization, can solve the problems of high risk and strictness of seed production failure, and achieves the effects of improving purity, stable flowering period fertility and increasing yield

Inactive Publication Date: 2009-03-25
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its Achilles heel is that it has stricter requirements on climatic conditions, there are not many seed production areas to choose from, and the risk of seed production failure is greater

Method used

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  • Seed selection method of cabbage type rape twofold sterile line

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The sterile line, code-named BronowskiNap CMS, was obtained by continuous backcrossing of the Canadian variety AC Elect by Bronowski.

[0034] Using an environmentally sensitive sterile line 373S selected and bred (China Agricultural Science Bulletin, 2007, (7): 255-258, this sterile line has been planted and identified in Qinghai Academy of Agriculture and Forestry Sciences, Yangzhou Institute of Agricultural Sciences, Shanxi Academy of Agricultural Sciences, etc. , and donated to relevant units to carry out breeding application research) to cross with the variety Zhongshuang 9, F 1 Daizai and Zhongshuang No. 9 were backcrossed with BCF 2 Environmentally sensitive sterile strains isolated from successive generations were continuously selfed to obtain the environmentally sensitive sterile line Z9-EGMS. The breeding line Z9-EGMS was sown in autumn in Yangling, Shaanxi and other places. The fertility performance of the flowering period is the same as that of 373S. It is ...

Embodiment 2

[0040] The temperature-sensitive male sterile two-line hybrid Xiangzayou No. 5 bred from the Crop Research Institute of the Hunan Academy of Agricultural Sciences (variety approval number: National Examination Oil 2003011, approved by the Hunan Provincial Crop Variety Approval Committee in 2002, the original code 9705 , Variety source: Xiang 91S × Zhongshuang 4. Chinese Journal of Oil Crops. 2005 (3): 74-76) an environmentally sensitive male sterile line Xiang5 S was isolated from the offspring.

[0041] The environmentally sensitive male sterile line Xiang5 S was used as the male parent to cross with the Nap CMS maintainer line Bronowski (glucosinolate2 Two environmentally sensitive sterile strains were isolated from 178 strains. Using these two environmentally sensitive sterile lines as male parents to testcross BronowskiNap CMS, one line can partially maintain CMS. Eleven plants were selected from the self-bred progeny of this environmentally sensitive sterile plant, and th...

Embodiment 3

[0042] Embodiment 3: Double sterile line and other sterile line comparative test of fertility

[0043] Experiments were carried out in the Yangling area of ​​Shaanxi Province, where the double sterile line Z9-ECMS, the double sterile maintainer line BZ9, the environmentally sensitive GMS line Z9-EGMS and Bronowski Nap CMS were sown in autumn.

[0044] 10 plants are randomly selected in the flowering period of the next year, and 10 fresh flowers in different parts are taken from each plant, the anthers are extruded to release the pollen, stained with acetic acid carmine, and the active pollen (ellipse in shape, complete in shape, capable of being dyed) is counted under a microscope. red) percentage.

[0045] Another 10 bagged plants were selected for self-fertilization, and the self-seed setting rate was calculated relative to the normal varieties Zhongshuang 9 and Bronowski, and the male fertility of the entire flowering period was estimated. The results showed that the ferti...

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Abstract

The invention discloses a breeding method for novel brassica napus dual-sterile lines. The method adopts the environment-sensitive genic sterile material to hybridize the Nap CMS-maintainer line; the offspring select the environment-sensitive genic sterile plant testcross to maintain Nap CMS; the environment-sensitive genic sterile gene is guided into the Nap CMS, and the bred dual-sterile lines are completely sterile, with flat petals, white pollen-free stamens and stable flowering fertility. Most normal varieties can resume the fertility, so as to greatly expand the utilization range of the rapeseed heterosis and reduce the seed production risk; moreover, the method can improve the purity of hybrid seed production and provides a technical support to increase the hybrid seed yield.

Description

technical field [0001] The invention belongs to the field of crop heterosis utilization methods, and relates to a method for breeding male sterile lines of rapeseed, in particular to a method for breeding dual male sterile lines of "environmentally sensitive nucleus + Nap cytoplasm" in Brassica napus. Background technique [0002] Cytoplasmic male sterility (CMS, including the "cytoplasmic-nuclear interaction" male sterility in the theory of three types of male sterility) is the main way to utilize heterosis in Brassica napus. There are three CMS systems, Ogura, Polima and Nap, which were discovered earlier and widely studied in the world. Ogura CMS is widely used in Europe and North America, but its restorer lines and restorer genes are protected by patents. Polima, Shaanxi 2A CMS, etc. are widely used in China, and their genetic mode, restoration relationship, and fertility performance all have the characteristics of Polima-type CMS (Yang Guangsheng et al., Genetic Classi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 于澄宇胡胜武
Owner NORTHWEST A & F UNIV
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