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Method for preparing corn starch total sugar powder

A technology of corn starch and powdered sugar, which is applied in the field of bioengineering, can solve the problems of low glucose conversion rate and long saccharification time, and achieve the effects of wide application prospects, improved conversion rate and shortened production cycle

Inactive Publication Date: 2011-06-22
JIANGNAN UNIV
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Problems solved by technology

However, a major problem in the production process is that the conversion rate of glucose in the saccharification process is not high, and the saccharification time is relatively long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the preparation of a kind of cornstarch whole sugar powder:

[0013] The cornstarch is configured into a 30%-40% starch slurry. with HCl or NaCO 3 Adjust the pH value of the slurry to 6.2-6.5. Add α-amylase 30U / g starch, and use the second injection liquefaction method for liquefaction. The first injection liquefaction temperature is 110°C, 45min, the second injection liquefaction temperature is 130°C, 15min, and the color reaction with iodine is negative. Indicates the end point of liquefaction. Then the temperature was raised to 140°C to inactivate the enzyme for 10 minutes. Then the temperature was lowered to 60°C. The pH was adjusted to 4.0-4.5 with HCl, the temperature was controlled at 60±1°C, the dosage of glucoamylase was 250u / g starch, and the dosage of pullulanase was 0.13U / g starch. When the saccharification time is 60 hours and the DE value is almost unchanged, the saccharification is terminated. Add 1% bentonite to the saccharified sugar...

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Abstract

The invention relates to a method for preparing corn starch whole powered sugar, belonging to the technical field of bioengineering. The method adopts Alpha-amylase and diastatic enzyme (glucoamylase) to hydrolyze corn starch at different times; during the saccharification process, pullulanase is adopted for synergistic reaction so that the DE value of the final saccharification of the product can be enhanced to 98.80 percent. The method provides a new technical basis for using the corn starch to prepare high-quality whole powdered sugar. By the simultaneous use of a plurality of diastatic enzymes, the conversion rate of the product is enhanced and the production cycle is shortened, thus opening up wide application prospect for realizing the production of the high-quality whole powdered sugar in China.

Description

technical field [0001] The invention discloses a preparation method of cornstarch whole sugar powder, which belongs to the technical field of bioengineering. Background technique [0002] Whole sugar powder is a kind of starch sugar product, which is starch sugar obtained after liquefaction and saccharification of starch without crystallization and nectar separation. However, a major problem in the production process is that the conversion rate of glucose is not high during the saccharification process, and the saccharification time is relatively long. After adopting the technology of enzymatic method, the conversion rate of starch saccharification reaches over 98%, and the DE value reaches over 98%. Glucoamylase is an exonuclease, which is very easy to decompose α-1, 4 glycosidic bonds, but very slow to decompose α-1, 6 glycosidic bonds, and 70% of the amylopectin in corn starch has With α-1,6 glycosidic bond. Pullulanase is a pullulanase, which has a strong effect on hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 江波张银志张涛
Owner JIANGNAN UNIV
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