Astragalus polysaccharide adjuvant and uses in type I riemerella anatipestifer inactivated vaccine

The technology of astragalus polysaccharide and Reesei bacteria is applied in the field of vaccine immune adjuvant and vaccine, which can solve the problem that duck infectious serositis has no immune effect, and achieve the prevention of duck infectious serositis, good safety and immunogen Sex, the effect of improving the immune effect

Inactive Publication Date: 2009-04-08
武汉市畜牧兽医科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The invention patent application with publication date of June 18, 2008 and publication number CN101200710A discloses a duck plague vacc...

Method used

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  • Astragalus polysaccharide adjuvant and uses in type I riemerella anatipestifer inactivated vaccine
  • Astragalus polysaccharide adjuvant and uses in type I riemerella anatipestifer inactivated vaccine
  • Astragalus polysaccharide adjuvant and uses in type I riemerella anatipestifer inactivated vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Inoculate the R. anatipestifer RA-1 strain on a TSA plate and inoculate it in TSB, culture it with shaking at 37°C for 18-24 hours, take the culture solution and inoculate it on TSA and blood agar plates, and use it as seeds after passing the purity inspection. solution, stored at 4°C;

[0022] (2), seed liquid is inoculated in TSB by 5% of the culture liquid volume, 37 DEG C ventilated and shakes and cultivates for 24 hours, adopts the plate counting method to carry out bacterial count after the pure inspection is qualified, simultaneously adds formaldehyde by 0.4% of the bacterial liquid volume The solution was inactivated at 37°C for 24 hours, and after inactivation, the concentration of the bacterial solution was adjusted to 1×10 10 CFU / mL, inoculated on blood agar and TSA plates, 5% CO 2 , Cultivate at 37°C for 48 hours, and those with aseptic growth are used to prepare vaccines;

[0023] (3), astragalus polysaccharide is made into the solution that concentra...

Embodiment 2

[0026] Step (1), (2) are identical with embodiment 1;

[0027] (3), astragalus polysaccharide is made into the solution that concentration is 20mg / ml;

[0028] (4), mix the inactivated bacterium liquid of R. anatipestifer RA-1 and the astragalus polysaccharide solution in a ratio of 1:1;

[0029] (5) Add 0.01% thimerosal of the total volume of the mixed solution to the mixed solution.

[0030] Materials and methods used in the present invention and the immune effect experiment are further described below:

[0031] 1 Materials and methods

[0032] 1.1 Materials

[0033] 1.1.1 Strains

[0034] The type I RA strain was isolated from a meat duck farm in the suburbs of Wuhan, and was freeze-dried after identification.

[0035] 1.1.2 Medium

[0036] Tryptic Soy Agar (Tryptic Soy Agar, TSA) and Tryptic Soy Broth (Tryptic Soy Broth, TSB) are products of Difco and Bacto, respectively, and 10% calf serum was added when used.

[0037] 1.1.3 Adjuvant raw materials

[0038] Astraga...

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Abstract

The invention relates to a vaccine immunologic adjuvant and a vaccine, in particular to an astragalus polyose adjuvant and application thereof in an I-riemerella anatipestifer inactivated vaccine. The vaccine adjuvant is an astragalus polyose solution with the concentration of 30 milligrams per milliliter. An astragalus polyose inactivated vaccine for the I-riemerella anatipestifer is prepared from a riemerella anatipestifer RA-1 inactivated bacteria liquid as the vaccine and the astragalus polyose solution as the adjuvant through mixing. The astragalus polyose adjuvant improves the immune efficacy of the prior inactivated vaccine, effectively prevents and controls infectious serositis of duck, and provides a basis for the research of a novel immunologic adjuvant. The vaccine has good safety and immunogenicity, and can effectively prevent the infectious serositis of duck.

Description

technical field [0001] The invention relates to a vaccine immune adjuvant and a vaccine, in particular to an astragalus polysaccharide adjuvant and its application in an inactivated vaccine of type I R. anatipestifer. Background technique [0002] Astragalus polysaccharide (APS) is the main natural active ingredient in the traditional Chinese medicine Astragalus, which can promote the immune function of the body, increase the number of white blood cells and lymphocytes, increase the transformation rate of lymphocytes and macrophage activity, and induce interferon. Astragalus polysaccharide is generally made into a pharmaceutical composition and used as a medicine for improving immunity. However, there is no report on its use as a vaccine adjuvant. Because poultry, especially ducks are susceptible to diseases, and the transmission speed is very fast, once ducks are infected with diseases, they often cause huge economic losses to farmers. Therefore, it is very necessary to c...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/39A61P31/04
Inventor 金尔光陈洁陈橙钱运国周木清宫时玉华娟童伟文吴建英
Owner 武汉市畜牧兽医科学研究所
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