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Immunity chromatography detection test paper for fussol poison and preparation method thereof

An immunochromatographic detection and fluoroacetamide technology, applied in the field of immunodetection, can solve problems such as being unable to adapt to high throughput, and achieve the effect of simple operation

Inactive Publication Date: 2009-04-08
JIANGNAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, these detection methods require expensive instruments and equipment, professional and technical personnel are required for operation, and cannot be adapted to high-throughput, on-site rapid detection

Method used

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  • Immunity chromatography detection test paper for fussol poison and preparation method thereof

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Embodiment 1

[0015] One end of the PVC backing is pasted with a sample pad and a gold standard pad in sequence, a nitrocellulose membrane is stuck in the middle, and an absorbent pad is pasted at the other end, and the gold standard pad is coated with a cluster-specific antibody-colloidal gold marker against fluoroacetamide poisons , The nitrocellulose membrane was coated with sodium fluoroacetate-OVA and goat anti-rabbit IgG.

[0016] Prepare as follows:

[0017] (1) Preparation of sodium fluoroacetate-OVA conjugate: Dissolve sodium fluoroacetate and OVA in PBS (0.01mol / L pH8) at a molar ratio of 50:1, add EDC cross-linking agent under stirring, react for 3 hours, and dialyze at 4°C After 3 days, sodium fluoroacetate-OVA conjugates were formed;

[0018] (2) Preparation of polyclonal antibody against sodium fluoroacetate: prepare sodium fluoroacetate-BSA conjugate with the above-mentioned EDC method, and obtain high-purity polyclonal antibody against sodium fluoroacetate by immunization w...

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Abstract

The invention provides an immunity-chromatography detection test paper strip of fluoroacetamide poison, and a preparation method thereof, belonging to the technical field of immunoassay. The method uses the combination of a cluster specific antibody of anti-sodium fluoroacetate and the color development matter colloid gold as the reagent strip of a detection reagent assembly and carries out the poisoning screening detection of fluoroacetamide poison; the cost is low and the operation is quick, with the time of only 5-10 minutes; the test paper strip is convenient for being carried along and is suitable for site detection; and the operation is simple and convenient and special technical personnel is not needed. The test paper strip and the method can be used for the poisoning screening detection of fluoroacetamide poison.

Description

technical field [0001] An immunochromatographic detection test strip for fluoroacetamide poisons and a preparation method thereof belong to the technical field of immunodetection, and specifically relate to the preparation of immunocolloidal gold reagents and test strips. Background technique [0002] Fluoroacetamide, sodium fluoroacetate, and fluoroacetic acid are all highly toxic fluoroacetamides, and their toxicity and poisoning symptoms are similar to tetramine. Fluoroacetamide poisons have become one of the most common poisons in criminal cases in my country. After fluoroacetamide enters the organism, it is metabolized in the body to produce fluoroacetate anion, so the inspection work finally comes down to the analysis of trace amounts of fluoroacetate anion in biological samples. Existing methods that can realize multiple poisoning detection are mainly instrumental analysis, including ion chromatography, capillary electrophoresis, and derivatized GC-NPD, GC-ECD, and G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 胥传来朱颖越陈伟彭池方刘丽强谢会玲
Owner JIANGNAN UNIV
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