Method for identifying Y excellent series hybrid rice germ plasm
A technology of hybrid rice and identification method, which is applied in the field of identification of Yyou series hybrid rice germplasm, and achieves the effects of short detection time, accurate identification and large quantity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0043] (1), screen out the primers that can be used for Y excellent series hybrid rice germplasm identification in the following way:
[0044] ①. In the Gramene database, 12 rice chromosomes were found, and 5 SSR primers were randomly selected from each chromosome.
[0045] ②. Find the RM164 of chromosome 5 from the SSR primer obtained in step ①. The DNA sequence of the RM164 is:
[0046]forward sequence TCTTGCCCGTCACTGCAGATATCC,
[0047] Reverse sequence GCAGCCCTAATGCTACAATTCTTC.
[0048] (2), using the primers obtained in the above step (1) to perform PCR amplification on the rice sample 07A1 and the rice sample 07A2 submitted for identification:
[0049] ①. Use the PCR system to carry out PCR amplification. The components of the PCR system used are:
[0050] 1 μl of DNA template with a concentration of 25ng / μl
[0051] 2 μl of 10×PCR buffer with a pH value of 8.3
[0052] 1 μl of dNTPs at a concentration of 2.5 mM
[0053] 0.2 μl of Taq enzyme at a concentration of 5 ...
Embodiment 2
[0069] (1), screen out the primers that can be used for Y excellent series hybrid rice germplasm identification in the following way:
[0070] ①. In the Gramene database, 12 rice chromosomes were found, and 10 SSR primers were randomly selected from each chromosome.
[0071] 2. From the SSR primers obtained in step 1., find RM164, RM18 of the 7th chromosome, and RM258 of the 10th chromosome of the primers that can be used to identify the Y excellent series hybrid rice germplasm, wherein,
[0072] The DNA sequence of RM164 of chromosome 5 is:
[0073] forward sequence TCTTGCCCGTCACTGCAGATATCC,
[0074] reverse sequence GCAGCCCTAATGCTACAATTCTTC,
[0075] The DNA sequence of RM18 on chromosome 7 is:
[0076] forward sequence TTCCCTCTCATGAGCTCCAT,
[0077] reverse sequence GAGTGCCTGGCGCTGTAC,
[0078] The DNA sequence of RM258 on chromosome 10 is:
[0079] Forward sequence TGCTGTATGTAGCTCGCACC,
[0080] Reverse sequence TGGCCTTTAAAGCTGTCGC.
[0081] (2), using the primers ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com