Construction method of microecosystem for microbial retting of ramie phloem

Abstract

The invention relates to a method for establishing a ramie phloem fiber biological degumming microecosystem, which comprises the following steps: bacterial sources are collected from a plurality of rotten ramie habitats; and ramie phloem is taken as a single carbon source and a single energy, subjected to enrichment culture for a short period and mixed with the bacterial sources in equal proportion; the ramie phloem is taken as the single carbon source and the single energy and subjected to directional acclimatization at a temperature of between 40 and 43 DEG C and at an initial pH value of 7.5; the ramie phloem is subjected to refueling and repeated inoculation when being separated into the shape of cotton, wherein the inoculum size is 10 percent of the total volume of a culture solution, and the accumulated acclimatization time is between 1 and 2 years; the degumming microecosystem is established after gradual removal of mixed bacteria; floras are subjected to cross-feeding in the system, and secrete extracellular enzymes to highly efficiently degrade the colloids; and the flora structure and the functional stability in the system are maintained by means of transfer of living bodies. The method has the advantages of quick speed of acquisition of high-efficiency degumming strains, extensive varieties of degumming enzymes, high appetency of the enzymes and ramie colloid substrates, strong mixed bacteria resistance of the floras, difficulty of causing degeneration of the degumming capacity, etc.

Description

technical field [0001] The invention belongs to the microbial degumming technology of hemp bast fiber, and in particular relates to a method for constructing a micro-ecosystem for biological degumming of ramie bast fiber. Background technique [0002] The ramie industry is the most characteristic traditional industry in China. China's ramie resources account for more than 95% of the world's share, and its output ranks first. Compared with chemical fibers, ramie bast fiber has the advantages of renewability, sanitation, antistatic and anti-ultraviolet rays, and also has a silk-like luster. It is a raw material for the textile industry that is friendly to humans and the ecological environment. With the concern of environmental protection, the global demand for ramie fiber fabrics is increasing year by year. However, due to the difficulty in ramie degumming and the lag in product technology development, the processing volume of ramie fiber is still less than 1% in the entire t...

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: for traditional pure culture degumming, the enzyme production ability is not high, the enzyme degumming ability is not strong, the strain preparation process requirements are high, easy to infect miscellaneous bacteria and strain degradation, etc., to provide a ramie toughness The construction method of the micro-ecosystem of leather fiber biological degumming, this method is to adopt the multi-strain joint degumming of mixed culture (referred to as mixed bacteria degumming), that is to use the long-term directional domestication of the flora in the ramie bast environment to highly specialized in ramie nutrition. Efficient degumming with monotrophic and symbiotic characteristics, using the occupation of the basic ecological niche of the degumming environment by the flora in the process of co-evolution to resist the contamination of bacteria, and using long-term maintenance of selective pressure to ensure the structural and functional stability of the degumming micro-ecosystem

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