32 results about "Collema" patented technology

The invention discloses a method for preparing a banana bast fiber by adopting biological degumming, which is characterized by comprising the following steps of: preprocessing, inoculating, fermenting, inactivating, stamping and postprocessing. The prepared banana bast fiber has soft and loose appearance and uniform color and has no taste or obvious flaws; the fiber length is 80mm-200mm, the fiber count is 300Nm-500Nm, the breaking strength of fiber bundles is 3.5g / D-5.0g / D, and the residual gum content is 1.5 percent-3.2 percent. The biologically degummed banana bast fiber effectively removes the non-spinnable components of colloid, lignin, hemicellulose and the like and keeps the bacteria proofing, rapid moisture absorption and release of the self characteristics of the fiber, thereby ensuring the strength of the fiber; meanwhile, the banana fiber is kneaded and rolled by sterilization and machines before degumming, thereby ensuring the degumming effect and the smooth going of degumming and enabling the degummed fiber to be beneficial to the production and production development of the next working procedure.

The invention discloses an astragalus seed coating and a coating method for the seed coating relating to a seed coating and a coating method for the seed coating which solves the problem that an astragalus as a medicinal plant can not use the exiting seed coating. The seed coating is essentially formed by 25 to 30 portions of bactericide, 5 to 10 portions of insecticide, 0.001 to 0.05 portions of plant modifying agent and 1 to 10 portions of accessory ingredient according to a weight portion. The coating method includes: 1.washing, dipping and rewashing the seeds; 2.sealing the seeds and drying; 3.arranging the astragalus seed coating in water to be stirred uniformly and then arranging the astragalus seeds in the mixture to be stirred uniformly, laying open and air-drying, namely finishing the coating of the astragalus seeds. The astragalus seed coating and a coating method for the seed coating of the invention are designed aiming at the growing characteristics and multiple plant diseases and insect pests during a seedling stage of the astragalus with deciduous rate less than 0.2 percent. The invention can enhance the seedlings and promote a root system with an astragalus root shoot ratio improved by 30 percent. The coating method for the astragalus seed coating of the invention can remove a cortical gelatinous layer of the astragalus seeds, break the seed capsule obstacle and meet the need on the temperature for the seed bourgeon by utilizing the pathogenicbacteria on the surface of the seeds and improve the astragalus bourgeon rate by 20 to 45 percentage points.

The invention discloses a tissue isolation method of edible fungus strains. The method comprises the following steps: (1) sterilizing and moistening dried edible fungus; (2) cutting a fungus tissue block; (3) sterilizing the fungus tissue block with alcohol lamp flame; (4) inserting the fungus tissue block in an isolation culture medium for thermostatic isolating culture; and (5) selecting new hypha as an inoculation block to be inoculated to a purified culture medium for purification culture, and obtaining the edible fungus strains. The tissue isolation method of the edible fungus strains has the advantages of simple operation, low production cost, high hypha isolation success ratio, fast hypha germination, strong growth vigor and the like. The method has wide application, and is applicable to tissue isolation of various jelly fungi.

The invention discloses a method for preparing viscose fibers by using pineapple leaves, comprising the following steps: firstly, sorting the pineapple leaves, and removing stings at the edge of each pineapple leaf; secondly, adopting mechanical knock or roll compacting, scraping mesophyll colloid of the pineapple leaves through a combing method, and obtaining crude pineapple leaf cellulose fibers through washing; thirdly, removing residual colloid in microorganism mould solution through fermentation treatment and hydrogen peroxide treatment; fourthly, preparing alkali cellulose; fifthly, yellowing and dissolving the alkali cellulose to manufacture spinning slurry; sixthly, defoaming the spinning slurry; and seventhly, spinning with a wet method. The invention has the advantages that firstly, waste pineapple leaves are fully utilized to prepare the viscose fibers, and the economic value of pineapples is enhanced; secondly, hydrogen peroxide is used to replace sodium sulfite, sodium bisulfite, ammonium oxalate and ammonium citrate in a method patent (ZL200510111703.2) of protobiont-chemistry, and drained waste water has no inorganic matter and is full of biodegradable polysaccharide compounds. Therefore, the invention more accords with the environmental requirements.

The invention relates to a method for preparing viscose fiber by sisal leaves. The method comprises the following steps: (1) sorting the sisal leaves; (2) adopting mechanical knocking or roll compacting, carrying out devillication with a carding method so as to scrap off diachyma colloid of the sisal leaves, carrying out water washing, and obtaining sisal leaves cellulose base fiber; (3) carrying out fermentation treatment in a microorganic mould solution and then peroxide treatment so as to remove residual colloid; (4) preparing soda cellulose; (5) etiolating and dissolving the soda cellulose to obtain filature serosity; (6) defoaming the filature serosity; and (7) wet spinning. The invention has the advantages that the method fully utilizes the waste sisal leaves to prepare the viscose fiber and improves the economic value of the sisal leaves; peroxide is used for replacing sodium sulfite, sodium bisulfite, ammonium oxalate, ammonium citrate in the ZL200510111703.2 biological-chemical method patent; and the discharged sewage contains no inorganic matters but polysaccharide compounds which are biologically degradable, and more conforms to the environment friendly requirement.

The invention discloses a novel tremella culture medium. The novel tremella culture medium is composed of, by mass, 60-75 parts of base stock, 20-30 parts of nutritional ingredients, and 1-3 parts of gypsum powder, wherein the nutritional ingredients include at least one of lotus seed powder and lotus nut powder, and the base stock includes one or more of pear wood bits, lotus seed hulls and cotton seed hulls. The invention further discloses a tremella cultivation method. By the adoption of the novel tremella culture medium and the tremella cultivation method, tremella unique in mouthfeel and flavor can be obtained. Due to the small colloid content, the tremella is crystal clear, smooth and crisp in mouthfeel and high in quality and is not burnt after being boiled for a long time. Furthermore, the obtained tremella has the unique flavor, cool bitter taste and delicate fragrance of lotus seeds.

The invention discloses a new process for preparing a kudzu vine rope. The new process comprises steps as follows: soaking kudzu vines, peeling the soaked kudzu vines, rubbing and washing peeled kudzu vine barks, soaking kudzu vine bark fiber in a wormwood extract liquid, dividing the soaked kudzu vine bark fiber, drying the divided kudzu vine bark fiber in the sun and weaving the divided kudzu vine bark fiber into the rope. Compared with a traditional process, the kudzu vine twined rope prepared with the technique has advantages as follows: a layer of pond silt is laid at the bottom of a soaking pond and is rich in flora capable of decomposing organic matters, the floras decompose colloid and other organic matters on the kudzu vine barks, the soaking time is shortened, the kudzu vine barks can be separated from lignin parts of the kudzu vines, and accordingly, follow-up peeling is facilitated; besides, the skin layer can be effectively decomposed due to decomposition of the organic matters, no black spots are left on the left kudzu vine fiber through follow-up rubbing and washing, and the quality of the kudzu wine fiber is improved.

The invention relates to a method for tanning Tan sheep skin with a nano softening enzyme, which comprises the following steps: pickling; dampening; adding 5-8% of nano softening enzyme into a rotating drum; after the rotating drum is rotated for 4-5 hours at ordinary temperature, heating to 40-50 DEG C, continuing rotating the rotating drum for 4-6 hours, and adding 0.5-1% of nano softening enzyme to regulate the pH value to 2.5; rinsing; washing out loose color; and washing with water. Under the actions of the nano softening enzyme and acidic condition, elastic fibers and excess colloids in the skin can be effectively dissolved and removed; and since the action of the nano softening enzyme is mild, the invention can ensure the low hair slipping tendency and fullness of the Tan sheep skin. In addition, no chromium or aluminum salt is used, thereby relieving the environmental pollution, reducing the control and routine maintenance cost for waste water and lowering the production cost.

The invention provides an organic / inorganic composite fertilizer prepared from molasses fermentation liquor. How to effectively treat molasses fermentation fertilizer liquor generated by sugar plants, alcohol plants, cassava starch plants, yeast plants, monosodium glutamate plants and the like has become an urgent environmental problem and a severe economic problem ever since 1980-1990; and how to change wastes into valuable substances, solve the environmental problems and bring in considerable benefits becomes a long-term troublesome problem of the technical field. Ferrous sulfate and aerobic bacteria are creatively combined to remove odor of the molasses fermentation liquor, dissolve part of colloids and maintain rich nitrogen, phosphorus, potassium, humic acid, amino acid and other necessary minor elements for plants; and degumming and spraying granulation are performed to obtain the solid organic / inorganic composite fertilizer which is low in cost and suitable for industrialized production, thereby opening up a brand-new industry for the technical field.

The invention discloses a biomass-charcoal-enveloped colloid-rich fertilizer and a preparation method thereof. The fertilizer is prepared from the raw materials of, by weight, 20-30 parts of rape straw, 25-40 parts of corn straw, 10-15 parts of agar powder, 2-3 parts of an EM inoculant, 10-20 parts of lotus pond slit, 20-35 parts of Chinese herbal medicine residue, 10-16 parts of poultry feather, 10-20 parts of potassium bicarbonate, 20-35 parts of a calcium-magnesium-phosphorous fertilizer, 6-10 parts of calcium chloride, 10-16 parts of sodium fulvate, 20-30 parts of ammonium chloride, 5-7 parts of an adhesive, and a proper amount of water. According to the invention, the Chinese herbal medicine residue, poultry feather and the like are shortly fermented with microbial strains, and the agar powder is added for improving soil colloid content. When colloid content is improved, soil particles can be cemented to form pellets, and water retention, fertilizer retention and compaction prevention capacities are improved.

The invention belongs to the field of microbial culture technology, relates to a process technology adopting a bacillus mucilaginosus industrial liquid to form spores, and particularly relates to a submerged fermentation culture medium of bacillus mucilaginosus applied to microbial fertilizers. The culture medium comprises the following raw materials according to the weight percentage: 0.9-1.5% of corn flour, 0.1-0.3% of soybean meal, 0.08-0.2% of dipotassium phosphate, and the balance being water. The culture medium is obtained by the steps of mixing the corn flour, the soybean meal, dipotassium phosphate and water, stirring evenly, sterilizing for 20-40 min at the temperature of 121 DEG C, and then cooling to 25-40 DEG C to obtain the culture medium. The pH value of the culture medium is in the range of about 6.0-7.5, and the spores of bacillus mucilaginosus grow well in the pH value of 6.0-8.0, so the culture medium can promote thalli growth and spore formation without using an acid or alkali to adjust pH before sterilization, simplifies operation, improves the product quality, reduces the production cost, and is more suitable for industrial mass production.

The invention relates to the technical field of genetic engineering, in particular to bacillus subtilis not producing cellulase as well as a construction method and application thereof. The construction method comprises the following steps: knocking out a cellulase Endoglucanase encoding gene eglS of the bacillus subtilis strain 168 to obtain the bacillus subtilis not producing the cellulase. Themutant strain obtained by the construction method can generate various kinds of degummase such as pectinase and hemicellulase, can directly decompose the colloid component in ramie bast, can be applied to microbial degumming of the ramie, has the advantages of no damage to ramie fiber and safety for people and livestock, and is favorable for protecting the ecological environment.

The invention relates to an environmental-friendly hollow brick made from the following raw materials in parts by weight: (1) internal brick body mortar, i.e. 100 parts of cement, 250-350 parts of sand, 50-150 parts of slag, 400-600 parts of water, 10-20 parts of chrysanthemum powder, 10-20 parts of 75 <#> industrial mildewcide, 0.1-0.5 part of early strength agent; and (2) coating sizing agents,i.e. 5-8 parts of gelatin, 2-3 parts of honey, 7-9 parts of glutinous rice flour, 4-6 parts of green bean powder, 2-4 parts of sulphur powder, 1-3 parts of boric acid powder and 20-30 parts of water.The invention also relates to the preparation method of the environmental-friendly hollow brick. The invention has the advantages of simple raw materials, low cost, heat preservation and isolation andlong service life; the coating sizing agents are ropy colloids, and soil layer sizing agents form a compact and solid shell after being dried, thereby effectively preventing the vast majority of harmful gas from being separated out; in addition, the chrysanthemum powder is added so that a small amount of the separated gas has fragrant smell, thereby reducing the harm on human bodies.

The invention relates to a method for establishing a ramie phloem fiber biological degumming microecosystem, which comprises the following steps: bacterial sources are collected from a plurality of rotten ramie habitats; and ramie phloem is taken as a single carbon source and a single energy, subjected to enrichment culture for a short period and mixed with the bacterial sources in equal proportion; the ramie phloem is taken as the single carbon source and the single energy and subjected to directional acclimatization at a temperature of between 40 and 43 DEG C and at an initial pH value of 7.5; the ramie phloem is subjected to refueling and repeated inoculation when being separated into the shape of cotton, wherein the inoculum size is 10 percent of the total volume of a culture solution, and the accumulated acclimatization time is between 1 and 2 years; the degumming microecosystem is established after gradual removal of mixed bacteria; floras are subjected to cross-feeding in the system, and secrete extracellular enzymes to highly efficiently degrade the colloids; and the flora structure and the functional stability in the system are maintained by means of transfer of living bodies. The method has the advantages of quick speed of acquisition of high-efficiency degumming strains, extensive varieties of degumming enzymes, high appetency of the enzymes and ramie colloid substrates, strong mixed bacteria resistance of the floras, difficulty of causing degeneration of the degumming capacity, etc.

The invention provides a triterpenoid-saponin compound, wherein the molecular formula is C52H84O21, and the chemical name is 3beta-O-{beta-D- xylopyranosyl-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-[beta-D-glucopyranosyl(1->4)]-alpha-L-arabinopyranosyl}hederagenin. In-vitro anti-tumor tests indicate that the compound has a remarkable inhibiting action for C6 rat glioblastoma cells as well as four kinds of U87MG, U251MG, BT325 and SHG44 glioblastoma cells without influencing the growth of primarily-cultured human neuroglia cells. The compound is hopeful to be used for preparing a medicament for treating a glioma.

The invention relates to a method for production of refined dried ramie by microbial blending and degumming, Bacillus Bacillus sp., HG-116 and Bacillus Bacillus sp., HG-247 are used as strains for respective strain activation step and enlarged cultivation step to obtain a bacterium fluid, and the bacterium fluid and water are mixed for fermentation cultivation, enzyme production cultivation and biological degumming to achieve the purpose of rapid and thorough removal of various ramie gum components. After optimization of types of culture mediums and fermentation conditions, two kinds of microorganisms can grow well, cultivation period is short, technical indexes of the produced refined dried ramie are far above the national standards of first-level refined dried ramie, the refined dried ramie is very fluffy due to super low residual gum rate, a spinning pretreatment process is simple, dust is significantly reduced, spinning end breakage rate is low, ramie yarn comprehensive production cost is reduced, and ramie yarn quality is improved significantly.

The invention belongs to the technical field of tertiary oil recovery utilizing microbes and metabolism thereof to improve the crude oil yield, and discloses a strain of exiguobacterium mexicanum 2a, which is preserved in China Center for Type Culture Collection. The preservation number of the strain is CCTCC M2014674. The invention also discloses applications of exiguobacterium mexicanum 2a. The strain can be used to degrade crude oil or solid paraffin and can also be used to prevent crystal deposition of paraffin. The maximal degradation rate of colloids and asphalt in crude oil can reach 72.8% and 57.9% respectively, the viscosity of crude oil can be reduced by 11.1%, the solubility of solid paraffin in n-hexane is prominently improved, the soluble paraffin accounts for 62.0 % of the total weight of paraffin; compared with a control group, the crystallized paraffin is reduced by 64.7%; the wax clearing rate and wax preventing rate reaches 77.1% and 96.9% respectively; the cells of exiguobacterium mexicanum 2a are highly hydrophobic, the cells can tightly adhere on the surface of metals to form a biological membrane, and thus the crystal deposition of wax is prevented.

The invention provides a preparation method of a banana fiber and hemp mattress, comprising the following steps of obtaining fibers from tree trunks that are cut after fruiting of banana trees every year by the following two ways: (1) feeding the tree trunks into a duel-roller machine with roller control for peeling of coarse barks and pectin to obtain the banana fibers; (2) stacking the tree trunks in the open air, naturally fermenting for 35 to 40 days and washing by using water to obtain the fibers; then placing the fibers under natural sunlight for drying or placing in a dryer of 100 to 105 DEG C for drying, and packaging for later use, wherein the moisture regain is 14% or below; then treating the extracted fiber raw material by adopting a biological or chemical treatment method; adding meldable fibers in the treated banana fibers, carrying out opening, impurity removal, carding and net formation, and carrying out hot melting and setting to prepare a blocky banana fiber elastic plate-type material; then according to design requirements, sewing and coating the blocky banana fiber plate-type material by a hemp fabric to finally prepare the banana fiber and hemp mattress. The mattress prepared by the method has the advantages of antibacterial effect and low price.

The invention discloses a composite microbial functional liquid fertilizer for dendrobe. The composite microbial functional liquid fertilizer is prepared from the following components in parts by weight: 4.0-4.1 parts of macroelements, 0.035-0.04 part of microelement, 0.05-0.08 part of iron salt chelate, 0.12-0.18 part of organic matters, 10-30 parts of microbial fermentation liquid and 950-990 parts of water. The composite microbial functional liquid fertilizer for the dendrobe contains microbial flora beneficial to plant growth, also contains balanced macroelements, microelements, pure natural biological factor, chelate and vegetable jelly, is capable of providing small and easy-to-absorb growth nutrients and forming a gelatinous membrane on the leaf surface, has water-holding and anti-burning effects and also has the function of inhibiting harmful microbes; use of a chemical antibacterial agent is avoided; the growth speed and the product quality of dendrobium officinale are greatly improved; and meanwhile, the composite microbial functional liquid fertilizer has the function of improving the activity of beneficial microelements of the soil.

The invention discloses an astragalus seed coating and a coating method for the seed coating relating to a seed coating and a coating method for the seed coating which solves the problem that an astragalus as a medicinal plant can not use the exiting seed coating. The seed coating is essentially formed by 25 to 30 portions of bactericide, 5 to 10 portions of insecticide, 0.001 to 0.05 portions ofplant modifying agent and 1 to 10 portions of accessory ingredient according to a weight portion. The coating method includes: 1.washing, dipping and rewashing the seeds; 2.sealing the seeds and drying; 3.arranging the astragalus seed coating in water to be stirred uniformly and then arranging the astragalus seeds in the mixture to be stirred uniformly, laying open and air-drying, namely finishing the coating of the astragalus seeds. The astragalus seed coating and a coating method for the seed coating of the invention are designed aiming at the growing characteristics and multiple plant diseases and insect pests during a seedling stage of the astragalus with deciduous rate less than 0.2 percent. The invention can enhance the seedlings and promote a root system with an astragalus root shoot ratio improved by 30 percent. The coating method for the astragalus seed coating of the invention can remove a cortical gelatinous layer of the astragalus seeds, break the seed capsule obstacle andmeet the need on the temperature for the seed bourgeon by utilizing the pathogenicbacteria on the surface of the seeds and improve the astragalus bourgeon rate by 20 to 45 percentage points.

The invention relates to a thermophilic and colloid degrading Bacillus licheniformis sp. QB26 and its application. The Bacillus licheniformis sp. QB26 involved in the invention has a preservation number of CGMCC No. 5250. can Being able to grow in thick oil and colloid under a temperature of 30DEG C-60DEG C, the strain has a degradation rate of over 45% to the colloid (20g / L), and can reduce the absolute content of the colloid in the thick oil (20g / L) by over 10%. The strain and its fermentation liquid can well emulsify colloid and thick oil, and can reduce the thick oil viscosity by over 66% when applied to an inorganic salt medium containing 50% thick oil. Further, the colloid and the thick oil can be degraded, and the thick oil viscosity can be reduced. Therefore, the strain provided in the invention has good effects in microbial enhanced oil recovery, and can enhance crude oil recovery by more than 14%.

The invention provides a preparation technology of grass carp balls, and relates to the field of food processing. The preparation technology of the grass carp balls comprises the following steps of slaughtering grass carps, carrying out thorough washing, removing fine fish bones from fish meat, and separating fish meat from fish bones; obtaining fish meat by carrying out scraping along muscle lines of the fish by using a knife; putting the obtained fish meat into a rinsing apparatus, adding a cleaning material so as to carry out rinsing until the fish meat is white, and taking the rinsed fishmeat out so as to obtain materials ready for using; adding seasonings into the fish meat, putting the seasoned fish meat into a mincing machine, and carrying out mincing so as to obtain minced fish meat ready for using; adding auxiliary materials into the minced fish meat, putting the mixture into a stirring machine, and carrying out stirring for 10-15 minutes so as to obtain materials ready for next process; putting the treated minced fish meat into a shaping machine, carrying out shaping, and cooking the shaped fish balls with hot water for 4-5 minutes so as to obtain cooked fish balls; andthen, carrying out packaging and refrigeration, and carrying out transportation. According to the preparation technology of the grass carp balls provided by the invention, most of fish blood and impurities are removed so that the prepared fish balls are free of fishy flavor; moreover, the fish balls are purely white in color, rich in colloid content, good in elasticity and toughness, as well as fresh and sweet in taste.

The invention discloses an insect pest resistant solid state fermentation gel type fertilizer additive and a preparation method thereof. The additive is prepared from the following raw materials in parts by weight: bacillus thuringiensis preparations, bacillus mucilaginosus microbial inoculums, lotus pool silt, 4mol / L NaOH solution, sweet potato peels, glucose, fishbone soup, ammonium bicarbonate, peat, humic acid powder, sweet potato stems, radix sophorae flavescentis, cortex meliae, garlic straws, pyrethrum cinerariifolium flowers, ginkgo leaves, vermiculite and a proper amount of water. Through the flocculation complexing effect of bacillus mucilaginosus, the effective period of the beneficial ingredients of plants in soil is greatly prolonged; great benefits are also achieved on the colloid formation of the soil.

The invention relates to the field of microorganisms, and particularly discloses a method for screening a degumming strain. The screening method comprises the following steps of: inoculating a sample containing pectinatus, bacillus or enterobacteria into an enriched medium for culturing; coating the cultured culture onto a primary screening culture medium for culturing; purifying a colony which produces a hydrolyzed circle on the primary screening culture medium; and inoculating the purified strain onto a secondary screening culture medium, culturing for 15-24 hours and testing the degumming rate of the strain, wherein a strain with the degumming rate over 80 percent is a screened target strain. In the method for screening the degumming strain, ramie juice is taken as a unique organic nutritional ingredient for screening, a strain for decomposing the gum ingredient of ramie can be obtained quickly and repeatedly, and the cellulose fiber ingredient of ramie is not damaged; and moreover, the strain has high degumming rate, high degumming speed and industrial application.

The invention relates to a method for production of refined dried ramie by microbial blending and degumming, Bacillus Bacillus sp., HG-116 and Bacillus Bacillus sp., HG-247 are used as strains for respective strain activation step and enlarged cultivation step to obtain a bacterium fluid, and the bacterium fluid and water are mixed for fermentation cultivation, enzyme production cultivation and biological degumming to achieve the purpose of rapid and thorough removal of various ramie gum components. After optimization of types of culture mediums and fermentation conditions, two kinds of microorganisms can grow well, cultivation period is short, technical indexes of the produced refined dried ramie are far above the national standards of first-level refined dried ramie, the refined dried ramie is very fluffy due to super low residual gum rate, a spinning pretreatment process is simple, dust is significantly reduced, spinning end breakage rate is low, ramie yarn comprehensive production cost is reduced, and ramie yarn quality is improved significantly.

The invention discloses an organic fertilizer for improving soil gluing water retention property of sandy ground and a preparation method thereof; the organic fertilizer is prepared from, by weight, zeolite, 2 mol / L Na2HPO4 solution, calcium chloride, cinnamomun camphcra leaf, sludge in culture pond, manioc waste, banana peel, cob, colloid bacillus fungicide, poultry feather, 5 mol / L dilute sulphuric acid solution, tofukasu, rice bran, fish meal and proper amount of water. According to the invention, the feather taking amino acid as the main component can be better degraded in later maturity stage; meanwhile, colloid bacillus is added to ferment together, thus the fermented maturity material has more gum contents, the stickiness is strong; water absorbing and fertilizer remaining ability are good; the organic fertilizer is very suitable for the planting environment with gravel mostly such as tea trees in hilly area.

The present invention provides Bacillus gibsonii S-2 strain for producing alkaline pectase in high yield and the solid fermentation process therewith to prepare pectase, and belongs to the field of biological engineering technology. The present invention produces alkaline pectase powder with waste biomass containing pectin as carbon source and inducer, added nitrogen source, phosphate, sodium carbonate and water, and through inoculating Bacillus gibsonii S-2 strain, culturing statically at about 35 deg.c while contacting air for 72 hr; water soaking the cultured matter to extract, filtering to obtain alkaline pectase liquid, ultrafiltering concentration to obtain concentrated pectase liquid and spray drying to obtain alkaline pectase powder. The enzyme activity is measured via DNS method, and the yield of the process reaches 3500 unit / g beet dreg.

The invention discloses a technology and method for treating organic waste water and particularly relates to a biological method for combined treatment based on Lemna aequinoctialis and photosyntheticbacteria and belongs to the technical field of waste water treatment. The method comprises (1) discharging organic waste water into an anaerobic acidification treatment tank and carrying out preliminary pretreatment for 18 to 24 hours, (2) after the pretreatment, discharging the waste water into a photosynthetic bacterium (PSB) treatment tank and carrying out treatment for 10-15d, (3) carrying out reflux on the waste water discharged by the PSB tank according to a reflux ratio of 30-60%, (4) discharging the remaining reflux waste water discharged by the PSB tank into a sedimentation tank andcarrying out treatment for 12-24h, (5) introducing the waste water in the sedimentation tank into a Lemna aequinoctialis treatment tank and carrying out secondary treatment for 10-20d, (6) periodically feeding fresh bacteria into the PSB treatment tank during the treatment, and (7) periodically recovering and reusing the sludge and Lemna aequinoctialis in treatment. The method is simple and environmentally friendly, is low in cost and has high efficiency. The removal rates of BOD, ammonia nitrogen and total phosphorus are 90% or more.