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Alkalophilic bacterium and production for solid state fermentation of alkali pectase from alkalophilic bacterium

An alkalophilic bacteria, solid state fermentation technology, applied in the field of bioengineering

Inactive Publication Date: 2007-08-22
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

We isolated and screened an alkaliphilic bacterium from alkaline soil, which has a high ability to produce alkaline pectinase. It has been identified as Bacillus gibsonii. So far, this strain has not been used for solid-state fermentation production A report on alkaline pectinase

Method used

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  • Alkalophilic bacterium and production for solid state fermentation of alkali pectase from alkalophilic bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Effects of different pectin-containing biomass and different humidity of medium on enzyme production

[0011] Add 1 times, 2 times, 3 times, 4 times water respectively in 5g (dry weight) containing pectin solid biomass, dissolve peptone 0.15g, potassium dihydrogen phosphate 0.23g, sodium carbonate 0.09g in every water, stir Uniformly, cultured statically at 35°C for 72 hours. The results are shown in Table 1.

[0012] Table 1: Effects of various pectin-containing biomass and medium humidity on enzyme production

[0013] pectin-containing biomass

[0014] Note: The enzyme activity is determined by the DN8 method, and one enzyme activity unit (u) is defined as: the enzyme required to degrade sodium polygalacturonate to produce 1 μmol of galacturonic acid per minute under the reaction conditions of 55°C and pH 10.5 amount.

Embodiment 2

[0015] Embodiment 2: the influence of different nitrogen sources on producing enzyme

[0016] Add 3 times the weight of the nutrient solution to the sugar beet meal, the composition of the nutrient solution is: 1.0% of different nitrogen sources, 0.15% of potassium dihydrogen phosphate, and 0.6% of sodium carbonate. Sugar beet pulp and nutrient solution were sterilized at 115°C for 15 minutes respectively, mixed uniformly at a weight ratio of 1:3, inoculated with liquid seeds, and cultured statically at 35°C for 72 hours. The results are shown in Table 2.

Embodiment 3

[0017] Embodiment 3: the impact of different sodium carbonate additions on producing enzyme

[0018] Add 15 milliliters of nutrient solution to 5 grams of sugar beet meal, and the composition of the nutrient solution is: 1.0% of yeast extract, 0.15% of potassium dihydrogen phosphate, and 0-1.5% of sodium carbonate. Sugar beet pulp and nutrient solution were sterilized at 115°C for 15 minutes respectively, mixed uniformly at a weight ratio of 1:3, inoculated with liquid seeds, and cultured statically at 35°C for 72 hours. The results are shown in Figure 1. When the sodium carbonate is 0.6%, the enzyme production rate is the highest, reaching 3500 units / g dry vegetable dregs.

[0019] Table 2: Effects of different nitrogen sources on enzyme production

[0020] nitrogen source

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Abstract

The present invention provides Bacillus gibsonii S-2 strain for producing alkaline pectase in high yield and the solid fermentation process therewith to prepare pectase, and belongs to the field of biological engineering technology. The present invention produces alkaline pectase powder with waste biomass containing pectin as carbon source and inducer, added nitrogen source, phosphate, sodium carbonate and water, and through inoculating Bacillus gibsonii S-2 strain, culturing statically at about 35 deg.c while contacting air for 72 hr; water soaking the cultured matter to extract, filtering to obtain alkaline pectase liquid, ultrafiltering concentration to obtain concentrated pectase liquid and spray drying to obtain alkaline pectase powder. The enzyme activity is measured via DNS method, and the yield of the process reaches 3500 unit / g beet dreg.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. technical background [0002] At present, alkaline pectinase is more and more widely used in the field of bioengineering, and its application fields include: biological refining of cotton fibers, degumming of plant bast fibers, papermaking, treatment of pectin wastewater, coffee and tea fermentation, detergents, etc. Chinese patent (publication number: CN1480529A) discloses a strain of Bacillus pumilus CCTCC No: M203042 to produce alkaline pectinase through liquid submerged fermentation, which can be used for scouring treatment of cotton textiles. A Chinese patent (publication number: CN1177003A) discloses that a strain of Bacillus alcalophilus CCTCCM96009 can be directly used for ramie degumming. Chinese patent (publication number: CN1113951A) discloses that a strain of Bacillus subtilis (Bacillus Subtilis) CCTCC No: 0213 produces alkaline pectinase through high-concentration liquid ferm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/24C12R1/07
Inventor 白志辉张保国李祖明张洪勋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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