Method for producing novel lance atractylodes rhizome inoculation agent

A kind of inoculant and Atractylodes inoculum technology, which is applied in the field of Atractylodes inoculum production, can solve the problems of difficulty in artificial cultivation, low seed germination rate, and poor stress resistance of Atractylodes atractylodes, so as to achieve easy control of production time, reduce agricultural environmental pollution, and increase yield easy to control effects

Inactive Publication Date: 2009-06-03
NANJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many places have begun to introduce and cultivate Atractylodes atractylodes, but because the continuous cropping obstacles of Atractylodes atractylodes can not be solved, there are still many problems in the development of Atractylodes atractylodes resources: (1) The germination rate of Atractylodes atractylodes seeds is low, which cannot provide stable seeds for large-scale planting. (2) Artificial cultivation is difficult, and the growth of Atractylodes atractylodes is slow. Both morbidity and mortality have increased. Wang Yixun and others investigated in Xiaochang County and Yingshan County, Hubei Province, an important base of standardized planting of Chinese herbal medicines (GAP), and found that the occurrence of Atractylodes atractylodes black spo...

Method used

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  • Method for producing novel lance atractylodes rhizome inoculation agent

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Embodiment 1

[0033]Activation of the strain and preparation of the seed solution: the strain Sclerotium sclerotium ATCC15200 (Sclerotium delphinii) was taken out from the refrigerator at 4°C, transferred to a PDA slope, grown at 25°C for 7 days, poured into sterile water, and the mycelium was scraped. Transfer to PDA liquid medium containing 1% by weight extract of Atractylodes atractylodes branches and leaves, ferment and cultivate for 7 days at 25° C. on a shaking table at 150 r / min. As seeds, use the GBCS-5 type full-automatic fermenter fermenter to ferment mycelium; 5L tank is filled with 3L fermentation liquid, and the inoculum size is 20%. Cultivate for 4 days to obtain a fermentation broth.

[0034] The fermented liquid is used as seed germination inoculant.

Embodiment 2

[0036] The strain Phomsis sp. was taken out from the 4°C refrigerator, transferred to a PDA slant, and grown for 7 days at 25°C, poured in sterile water, scraped the hyphae, and transferred to the PDA liquid medium. 25°C, shaker 150r / min fermentation culture for 7 days. Insert solid fermentation medium (2% of bran, 10% of Atractylodes atractylodes stalk, 47.5% of rice husk, 0.5% of inorganic salt, 40% of water), solid fermentation is about 10 days, and water retaining agent (high Water-absorbent resin) added to the material and mixed evenly.

[0037] This product is used as an inoculant in soil.

Embodiment 3

[0039] The fungus Cunninghamella echinulatathaxter ACCC31576 (Cunninghamella echinulatathaxter) was taken out from the refrigerator at 4°C, transferred to a PDA slope, grown at 25°C for 7 days, poured in sterile water, scraped off mycelium, and transferred to add a small amount of Atractylodes atractylodes branches and leaves The PDA liquid medium of the extract was fermented and cultured for 7 days at 25° C. in a shaking table at 150 r / min as fermented seeds in a fermenter. Utilize GBCS-5 automatic fermenter; 5L tank is filled with 3L fermentation broth, inoculum size: 20%. For aerobic fermentation, add 1% agar or gelatin as a thickener after the fermentation and culture, and stir evenly.

[0040] This fermented liquid is used for transplanting and soaking roots.

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Abstract

The invention relates to the field of agricultural seeds, in particular to a method for producing a lance atractylodes rhizome inoculation agent from lance atractylodes rhizome endophytic fungus or probiotic bacteria. The method specifically comprises the following steps: activating the lance atractylodes rhizome endophytic fungus or the probiotic bacteria; adding a small quantity of thickening agents or water retention agents after the lance atractylodes rhizome endophytic fungus or the probiotic bacteria are cultivated on a culture medium containing potato immersion liquid, lance atractylodes rhizome immersion liquid and glucose liquid or a solid medium containing lance atractylodes rhizome straws; and soaking lance atractylodes rhizome seeds and transplanting soaking roots or adding the seeds to rhizoplane soil directly. The inoculation agent is not easily influenced by the environment during the production and has short production period, simple production process, production time, easy control of output and remarkable action. The method adopting the soaking seeds, soaking plant roots or pouring and directly adding the seeds in soil ensures that the inoculation agent acts on plants and has the advantages of simple method, easy operation, less consumption, low cost and good effect.

Description

technical field [0001] The invention belongs to the technical field of agricultural application microorganisms, and in particular relates to a method for producing an inoculant of Atractylodes atractylodes. The bacterial agent produced by the method can overcome or eliminate obstacles to continuous cropping of Atractylodes atractylodes. Background technique [0002] Atractylodes lancea (Thunb.) DC. is a perennial medicinal plant of the genus Atractylodes lancea commonly used in Chinese folk. It was first recorded in "Shen Nong's Materia Medica". It has the effects of drying dampness and invigorating the spleen, expelling wind and cold, and improving eyesight. Atractylodes atractylodis is a famous authentic medicinal material in Jiangsu Province. It has a long history of clinical use in traditional Chinese medicine. Its active ingredients are mainly β-cineol, atractylone, atractylodes alcohol and polyacetylene component atractylodes. Arthralgia, anemofrigid cold and so on. ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00A01C1/06C12N1/14C12R1/645
Inventor 戴传超张波鞠群
Owner NANJING NORMAL UNIVERSITY
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