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Seed selection method of fertility-stable Keng rice(O.satival.ssp.keng) Dian-1 type sterile line at different ecological condition

A technology with ecological conditions and stability, applied in the agricultural field, can solve problems such as complex climatic conditions, and achieve the effects of preventing impurities and maintaining purity, improving efficiency and sterility stability.

Inactive Publication Date: 2011-03-23
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the special geographical location and complex climatic conditions, there is no perfect and effective method for improving the breeding of Dian I type japonica CMS lines.

Method used

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  • Seed selection method of fertility-stable Keng rice(O.satival.ssp.keng) Dian-1 type sterile line at different ecological condition
  • Seed selection method of fertility-stable Keng rice(O.satival.ssp.keng) Dian-1 type sterile line at different ecological condition
  • Seed selection method of fertility-stable Keng rice(O.satival.ssp.keng) Dian-1 type sterile line at different ecological condition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The experiment was set in Kunming, Yunnan (1,900 meters above sea level) in the warm and cool area and Mengzi (1,300 meters above sea level), Yunnan in the warm area, and 10 CMS materials were set up for treatment, namely: Kunming, Yunnan in the cool and warm area with different ecological conditions (1900 meters above sea level) and Yunnan Mengzi (1300 meters above sea level) in the warm area sow and plant CMS materials in different places in the same season; The planting points in Mengzi, Yunnan (1300 meters above sea level) in warm and warm regions were bagged with 6 bags each, and self-fertilization was investigated when they matured; Take 5-10 middle and upper glumes that are about to bloom the next day for each material at the planting point in Mengzi, Yunnan (1300 meters above sea level), and fix them in 75% alcohol solution for later use; Mash it with tweezers on the glass slide, and then use 0.75% I 2 -KI solution staining, put it under a microscope, magnify 1...

Embodiment 2

[0017] The experiment was set up in Anning (1850 meters above sea level) in the warm and cool area of ​​Yunnan and Gejiu (1200 meters above sea level) in the warm area, and 10 CMS materials were set up for processing, that is, Anning ( 1,850 meters above sea level) and Gejiu (1,200 meters above sea level) in warm regions were sown and planted with CMS materials in different places in the same season; Gejiu (1,200 meters above sea level) in the hot region was bagged with 6 bags each, and self-fertilization was investigated when it matured; during the flowering period of the CMS, it was grown in Anning (1,850 meters above sea level) in the cool region of Yunnan and Gejiu in the warm region of Yunnan (1,200 meters above sea level) Take 5-10 middle and upper glumes that are about to bloom the next day for each material at the planting point, and fix them in 75% alcohol solution for later use; during microscopic examination, take out the anthers, and use tweezers on the glass slide ...

Embodiment 3

[0021] The experiment was set in Songming, Yunnan (1,870 meters above sea level) in the warm and cool area and Jianshui (1,250 meters above sea level) in the warm area, and 10 CMS materials were set up for treatment, that is, using Yunnan in the cool and warm area with different ecological conditions. Songming (1,870 meters above sea level) and Yunnan Jianshui (1,250 meters above sea level) in the warm area were planted with CMS materials in different places in the same season; 1,870 meters) and Yunnan Jianshui (1,250 meters above sea level) planting sites in warm areas were bagged with 6 bags each, and self-fertilization was investigated when they matured; during the flowering period of the CMS, in Songming, Yunnan (1,870 meters above sea level) in warm and cool areas In the Jianshui (1250 meters above sea level) planting point in warm and warm regions, take 5 to 10 grains of the middle and upper glumes that are about to bloom the next day for each material, and fix them in 75...

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Abstract

The present invention pertaining to the field of agriculture relates to a Dian-1 type hybrid japonic sterile line having a stable interfertility in different ecological conditions. In order to maintaining that the current sterile line breeding method is the same as the routine method, the technical scheme of the present invention adopts a method of seeding and planting materials of the sterile line in different places having different ecological conditions in a same reason, wherein, the different places are a warm cool region with an altitude of 1800-1900m and a warm hot region with an altitude of 1200-1300m; before the sterile line is blooming, each material is sleeved by six bags for the self-fructification expedition; in the blooming period of the sterile line, each sterile line material is performed with pollen microscopic examination, and the change of stained abortion pollen is analyzed; the selection is determined according to the existence or not of self-fructification and thechange of stained abortion pollen. The functional effect of the present invention comprises fully assuring the characteristics of the sterile line materials, breeding the Dian-1 type hybrid japonic sterile line having a stable interfertility and strong ecological fitness by using the solid weather condition in Yunnan and improving the efficiency of selection of the Dian-1 type hybrid japonic sterile line.

Description

Technical field: [0001] The invention relates to a method for breeding Dian I type japonica sterile lines with stable fertility under different ecological conditions, belonging to the field of agriculture. Background technique: [0002] The male sterility of plants is mainly manifested through pollen abortion. Pollen fertility is a key index for evaluating the sterility and fertility of sterile lines, and there are obvious differences among male sterile lines of different plastid sources. Theoretically speaking, pollen fertility is shown earlier than seed setting rate and is less affected by environmental conditions; genetically speaking, pollen directly reflects the development status of male gametes, and pollen fertility is the most fundamental and most important measure of plant fertility. direct indicators. Through the determination of heritability, the pollen fertility rate has the highest heritability, the natural seed setting rate is second, and the bagging seed sett...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04
Inventor 黄大军谭学林洪汝科文建成张忠林金寿林谭亚玲
Owner YUNNAN AGRICULTURAL UNIVERSITY
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