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A kind of rapid propagation method of hybrid orchid seedlings of Molan and Sagittarius

A technology for hybrid orchid and seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low reproduction coefficient, long cycle, and no protocorm obtained, and achieves simplified cultivation process, convenient material collection, shortened time The effect of incubation time

Inactive Publication Date: 2018-03-09
SHANGHAI ACAD OF AGRI SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Cao Shoujin carried out tissue culture research using the tip, stem segment and leaf of the tiger head orchid as explants, and the results showed that: suitable protocorm induction medium: KC+5mg / L 6-BA+0.5 (or 1.0)mg / L NAA+0.5~1% sucrose; the protocorm induction rate of the shoot tip is 38~75%; the protocorm induction rate of the stem segment is 48~78%; the protocorm induction rate of the leaf is 5~16%; proliferation medium: KC+5mg / L BA+0.1 (or 0.5) mg / L NAA+0.2 peptone; the fresh weight increase rate of protocorms is 50-58% (Cao Shoujin, Northern Horticulture, 2007 (3): 167-169), but, This induction medium is used in the tissue culture of the hybrid orchid seedlings of Molan and tiger head orchid, and no protocorms are obtained.
[0007] "Red Beauty" is a hybrid variety of Molan Hongyingji and Tiger Head Orchid. It has a beautiful plant shape, a long flowering period, and a combination of large flowers, flower fragrance, and beautiful flower color. At present, its propagation method is mainly conventional branch propagation. Only 1 to 3 times the multiplication rate can be obtained every year, the reproduction coefficient is low, and the cycle is long

Method used

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  • A kind of rapid propagation method of hybrid orchid seedlings of Molan and Sagittarius
  • A kind of rapid propagation method of hybrid orchid seedlings of Molan and Sagittarius
  • A kind of rapid propagation method of hybrid orchid seedlings of Molan and Sagittarius

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Embodiment 1

[0038] A rapid propagation method of hybrid orchids of black orchid and tiger head orchid, comprising the following steps:

[0039] 1) Induction culture

[0040]Take the aseptic seedlings of "Red Beauty", the hybrid offspring of Molan and tiger head orchid, cut off the roots and top leaves, take 0.6cm of base tissue, peel off the outer 1-2 layers of leaf sheaths, and inoculate in induction medium; carry out light culture, Cultivate 50d, obtain hybrid orchid protocorm (referring to figure 1 ), the protocorm induction rate reached 83.3%;

[0041] Among them, the induction medium is: MS+6-BA 5.0mg / L+KT 1.0mg / L+NAA1.0mg / L+PIC 0.3mg / L+hydrolyzed casein 1.0mg / L+activated carbon 0.1g / L+sucrose 30g / L+ Agar powder 6.0g / L, pH=5.6;

[0042] 2) Proliferation culture and differentiation

[0043] Take the hybrid orchid protocorm obtained in step 1), cut it, and inoculate it in the protocorm proliferation and differentiation medium. After 20 days of cultivation, the protocorm begins to p...

Embodiment 2

[0051] A rapid propagation method of hybrid orchids of black orchid and tiger head orchid, comprising the following steps:

[0052] 1) Induction culture

[0053] Take the aseptic seedlings of "Red Beauty" which is a hybrid offspring of Molan and tiger head orchid, cut off the root and top leaves, take 0.8cm of base tissue, peel off the outer 1-2 layers of leaf sheaths, and inoculate in induction medium; carry out light culture, After culturing for 53 days, hybrid orchid protocorms were obtained, and the protocorm induction rate reached 75.0%;

[0054] Among them, the induction medium is: MS+6-BA 4.0mg / L+KT 1.5mg / L+NAA1.0mg / L+PIC 0.2mg / L+hydrolyzed casein 1.0mg / L+activated carbon 0.4g / L+sucrose 30g / L+ Agar powder 6.0g / L, pH=5.6;

[0055] 2) Proliferation culture and differentiation

[0056] Take the hybrid orchid protocorm obtained in step 1), cut it, and inoculate it in the protocorm proliferation and differentiation medium. After 20 days of cultivation, the protocorm begin...

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Abstract

The invention discloses a rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum. Hybrid cymbidium aseptic seedling base tissue of the cymbidium sinense and the cymbidium hookerianum is used as a raw material, induction culture is performed in an induction culture medium containing various plant growth regulators and additives, protocorms are obtained and subjected to proliferation and differentiation culture, and then seedling strengthening, rooting and transplantation are performed. Steps of proliferation and differentiation of the protocorms are combined, the culture procedure is simple, the material cost for preparation of the culture medium is greatly reduced, the labor cost for preparation of the culture medium and inoculation is also reduced, the inductivity of the protocorms is high, the quantity of the protocorms is large, the proliferation coefficient is large, and the method has multiple advantages of low production cost, high seedling quality, short culture period, avoidance of variation and the like.

Description

technical field [0001] The invention belongs to the technical field of orchid plant tissue culture, and in particular relates to a method for rapid propagation of hybrid orchid seedlings of black orchid and tiger head orchid. Background technique [0002] Cymbidium sinense is a perennial herbaceous flower of the genus Cymbidium in the family Orchidaceae. It is one of the five traditional Chinese orchids. Its leaves are elegant and beautiful, its petals are fragrant, and its colors are changeable. The flowering period is from winter to early spring. , has high ornamental value and economic value. Because it mostly blooms around the Spring Festival, it is also called Baosuilan. The traditional division propagation method has a very low reproduction coefficient, and generally can only multiply by 1 to 3 times per year. [0003] Tiger head orchid (Cymbidium hookerianum) is an epiphytic perennial herb of the genus Orchidaceae. Originated in Southwest China, it is mainly distrib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 朱建军殷丽青张春英李秀芬孙翊范晓芬张治忠
Owner SHANGHAI ACAD OF AGRI SCI
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