Microbe ground water seepage action clogging observation method and apparatus

Abstract

The invention discloses a method for observing obstruction of microbial groundwater seepage and blockage as well as a device thereof. The method comprises the followings steps: (1) adding a porous medium to an experiment box; (2) regulating water level of an upstream control water tank to stabilize the water flow, constantly illuminating with a light source, and recording light rays from the box; (3) recording the water level and nutritive substances of the control water tank by a digital camera and storing the images; (4) quantitatively analyzing microbial aggregation density and porous medium density, obtaining a sample containing microbe aqueous solution and the porous medium from a sampling valve of the experiment box; and (5) killing microbes by the ultraviolet ray; wherein, the experiment box is equipped with a valve, a filter screen and a water storage tank which is connected with a water pump, the water pump is connected with the control water tank, and a water inlet tube is connected with the experiment box. The method helps directly observe and record the propagation and migration of the microbes in the porous medium, the blockage manner and change of a water flow passage, obtains the observation data, facilitates the control of the microbial activity and sampling analysis, and is applicable to simulation research of the microbial seepage and obstruction problem in a groundwater source heat pump system.

Description

technical field [0001] The invention relates to the technical field of observation of biological clogging of groundwater seepage, more particularly to a method for observing microbial seepage clogging, and also relates to a method for studying the propagation, migration and clogging of groundwater by microorganisms in the water-bearing underground sand layer in the sand layer gap. Observation device for seepage phenomena. Background technique [0002] The reproduction and migration of microorganisms in natural water-bearing porous media will cause changes in the permeability properties of the media, which will adversely affect technical systems involving groundwater seepage. For example, the groundwater source heat pump system is a heat exchange system commonly used in areas with groundwater. During use, groundwater is used to exchange heat and provide a cold source or heat source for the ground air conditioning system, which involves microbial blockage. The low efficiency ...

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Problems solved by technology

However, such methods and devices estimate the content of microorganisms in the aqueous solution based on the overall permeability of the filling medium, which cannot provide direct observation of the specific migration process of microorganisms in the water flow area, and cannot provide sufficient data for the study of the mechanism of microbial blocking

Other similar devices have the disclosed upflow anaerobic biofilm reactor of the Chinese utility model patent (publication number CN2626986Y), which is a reactor for biochemical reactions, and the disclosed horizontal rotary biofilm reactor of the Chinese invention patent (publication number CN1246231C). The flow-suspended carrier biofilm reactor and its in-situ repair method for water body use microorganisms to degrade pollutants in water. Due to different design purposes, they cannot control the reproduction, migration and blockage of microorganisms in the water flow channel of the porous medium, and the changes in the water flow channel affect the microorganisms. Direct observational studies of the effects of

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