Electro-chemical method and system for human blood type detection

A detection system, electrochemical technology, applied in the direction of measuring devices, scientific instruments, biological testing, etc., can solve the problems of personnel or environmental pollution, low sensitivity, high requirements for reagent storage, etc., to achieve simple and convenient operation, high sensitivity, and avoid subjective interference Effect

Inactive Publication Date: 2009-07-01
INTEC PROD INC
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Other blood group detection methods also have their own shortcomings, such as the time-consuming neutralization experiment and low sensitivity
Dissociation experiments and mixed agglutination reactions require high operating requirements, and each experiment needs to repeatedly explore the conditions, and its sensitivity is not high; synergistic agglutination reactions use IgG antibodies to sensitize SPA proteins, and determine blood types through the agglutination of staphylococci, but the reagents High storage requirements limit its application; although radioimmunoassay and enzyme-linked immunoassays have high sensitivity, they are not suitable for single-person testing, and cannot achieve on-site testing
[0007] Th...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electro-chemical method and system for human blood type detection
  • Electro-chemical method and system for human blood type detection
  • Electro-chemical method and system for human blood type detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Detection of blood type by AC impedance method:

[0021] (1) Immobilize blood group antibodies (anti-A and anti-B antibodies) on the electrodes:

[0022] Weigh 0.01 g of chitosan solid, dilute to 1 ml with 0.2 M acetic acid solution, and the final concentration is 1%. Draw 1 μl of the above chitosan solution with a micropipette, and drop it on the 4.5mm 2 Graphite working electrode, and air-dried at room temperature.

[0023] Drop 0.05% glutaraldehyde solution on the surface of the treated working electrode overnight, rinse with 5mM phosphate buffered saline (PBS) and soak in the blood group antibody (anti-A, anti-B antibody) solution with a concentration of 5mg / ml, alternately Contact 24 hours. Take it out, wash away free blood group antibodies (anti-A, anti-B antibodies) with 5mM PBS, and finally block with 5% bovine serum albumin (BSA) for 2 hours. Store in a sealed container after drying.

[0024] (2) Add red blood cells to the sample to be tested...

Embodiment 2

[0030] Embodiment 2: the practical application of blood type detection method of the present invention

[0031]The three-electrode AC impedance method blood type detection system is suitable for street blood collection and home ABO blood type self-test. Through the pre-modified electrodes, it can quickly and easily identify the blood group. The method is simple and quick to operate, does not need additional reagents such as antihuman globulin, and the entire detection process can be completed in less than 10 minutes, and is easy to popularize. We use the traditional blood type detection method and the electrochemical blood type detection method of the present invention to simultaneously perform blood type detection on 100 individuals (blood donors) from the general population. The result shows that, compared with the detection result of the method and the known hemagglutination method, the coincidence rate reaches 100%. At the same time, the electrochemical detection method ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for checking human blood groups, in particular to a method for checking human ABO blood groups via electrochemical methods and a checking system. The system is composed of an electrode system and a current resistance checking system. The method comprises: adding an object sample on a modified electrode; according to the change of the alternative current resistance caused by the reaction between the red blood cells in the blood sample and the blood type antibodies fixed on the surface of the electrode, judging the blood groups. The method has simple operation and clear result, which can effectively avoid the subjective effect of operators.

Description

field of invention [0001] The invention relates to a method and system for detecting human blood type, in particular to an electrochemical detection method and system for detecting human ABO blood type by electrochemical means. Background of the invention [0002] Blood typing is the conventional method of dividing human blood into four different types, A, B, AB, and O. Currently, blood typing methods are based on cell aggregation of red blood cell suspensions. In this method, red blood cells are first isolated from the patient's serum. Mix an appropriate amount of erythrocytes with the test solution (containing anti-A serum, anti-B serum) or its dilution introduced into the test tube or on the glass slide, and then add reagents that facilitate the immune reaction and incubate, centrifuge and stir. Then visually observe under a microscope to determine whether the cells have aggregated. [0003] If the sample undergoes cell aggregation in the presence of anti-A reagent, bu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/80G01N27/416
Inventor 汪大明杨文肖江群王保丹
Owner INTEC PROD INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap