Combined inactivated vaccine for against infectious brunchitis and newcastle disease and preparation method thereof

A dual inactivated vaccine, bronchitis technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, virus antigen components, etc. Protective effect, broad antigen spectrum, good immunogenicity effect

Active Publication Date: 2009-07-15
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. There are many serotypes of infectious bronchitis virus, which brings great difficulties to immune prevention. A single serotype vaccine can only produce immunity to the same type of infectious bronchitis virus, but not to other types of infectious bronchitis virus. The virus can only provide partial protection or no protection at all. For example, the vaccines prepared by the H52, H120, and M41 strains currently in use cannot provide protection against the infectious bronchitis virus th...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of Chicken Infectious Bronchitis and Chicken Newcastle Disease Dual Inactivated Vaccine

[0043] (1) Preparation of poison for production

[0044] Inoculate 10-day-old SPF chicken embryos with 0.1ml / inoculation amount of infectious bronchitis virus Beijing A2 strain seed virus without dilution, collect embryos for about 40 hours, place them in a refrigerator at 4°C overnight, collect allantoic fluid, and filter it as Production with poison.

[0045] Dilute Newcastle disease GM strain virus by 10 4 Inoculate 10-day-old SPF chicken embryos at an inoculation volume of 0.1ml / piece, harvest dead embryos at about 48 hours, place them in a refrigerator at 4°C overnight, collect allantoic fluid, and filter it as poison for production.

[0046] (2) Toxic inactivation for production

[0047]The poisonous Beijing A2 strain and GM strain used for production were respectively inactivated with 0.5‰ formaldehyde solution at 37°C for 16 hours, and shaken 4-5 ti...

Embodiment 2

[0064] Example 2 Preparation of Chicken Infectious Bronchitis and Chicken Newcastle Disease Dual Inactivated Vaccine

[0065] (1) Preparation of poison for production

[0066] Inoculate 10-day-old SPF chicken embryos with 0.1ml / inoculation amount of Beijing A2 strain of infectious bronchitis without dilution, collect embryos for about 40 hours, place them in a refrigerator at 4°C overnight, collect allantoic fluid, and filter it as production Use poison.

[0067] Dilute Newcastle disease GM strain virus by 10 4 Inoculate 10-day-old SPF chicken embryos at an inoculation volume of 0.1ml / piece, harvest dead embryos at about 48 hours, place them in a refrigerator at 4°C overnight, collect allantoic fluid, and filter it as poison for production.

[0068] (2) Toxic inactivation for production

[0069] The poisonous Beijing A2 strain and GM strain used in production were respectively inactivated with 1‰ formaldehyde solution at 37°C for 24 hours, and shaken 4-5 times in the middle...

Embodiment 3

[0075] Example 3 Preparation of Chicken Infectious Bronchitis and Chicken Newcastle Disease Dual Inactivated Vaccine

[0076] (1) Preparation of poison for production

[0077] Inoculate 10-day-old SPF chicken embryos with 0.1ml / inoculation amount of Beijing A2 strain of infectious bronchitis without dilution, collect embryos for about 40 hours, place them in a refrigerator at 4°C overnight, collect allantoic fluid, and filter it as production Use poison.

[0078] Dilute Newcastle disease GM strain virus by 10 4 Inoculate 10-day-old SPF chicken embryos at an inoculation volume of 0.1ml / piece, harvest dead embryos at about 48 hours, place them in a refrigerator at 4°C overnight, collect allantoic fluid, and filter it as poison for production.

[0079] (2) Toxic inactivation for production

[0080] The poisonous Beijing A2 strain and GM strain used in production were respectively inactivated with 0.7‰ formaldehyde solution at 37°C for 20 hours, and oscillated 4-5 times in the ...

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PUM

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Abstract

The invention belongs to the technical field of veterinary biology new medicine and relates to a chicken infectious bronchitis and chicken new castle disease bigeminal inactivated vaccine and a preparation method thereof. Virus seeds used by the vaccine are respectively a chicken infectious bronchitis virus Beijing A2 strain and a chicken new castle disease virus GM strain. The vaccine is prepared by the steps of mixing the chicken infectious bronchitis virus Beijing A2 strain and the chicken new castle disease virus GM strain after respectively being inactivated by an alkylating agent, adding oil adjuvant and emulsifying. The bigeminal inactivated vaccine can play a good role in preventing the novel serological type virus of infectious bronchitis and the novel antigen virus of new castle disease and solve the problem that the two viruses are only partially protected or even not protected by the prior vaccine in many places in China, thereby the occurrence of the chicken infectious bronchitis and the chicken new castle disease is effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biological medicines, and relates to a double inactivated vaccine of chicken infectious bronchitis and chicken Newcastle disease and a preparation method thereof. Background technique [0002] Chicken infectious bronchitis is an acute and highly contagious infectious disease caused by chicken infectious bronchitis virus. Quality drops. In the early 1990s, a variant infectious bronchitis with respiratory symptoms, kidney lesions and muscle necrosis broke out in the UK, mainly harming broiler breeders and laying hens. The strain is called 793 / B or 4 / 91. [0003] Newcastle disease is a highly contagious disease caused by Newcastle disease virus, which can infect various poultry, mainly chickens and turkeys. The disease can occur throughout the year, with the highest incidence in autumn, winter and early spring each year, mainly affecting the nervous system, respiratory system and digestive s...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P11/00A61K39/17A61K39/215
Inventor 倪娇赵亚荣许秀梅张渊魁赵建增张国中赵继勋
Owner 兆丰华生物科技(南京)有限公司
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