Combined inactivated vaccine for against infectious brunchitis and newcastle disease and preparation method thereof

Abstract

The invention belongs to the technical field of veterinary biology new medicine and relates to a chicken infectious bronchitis and chicken new castle disease bigeminal inactivated vaccine and a preparation method thereof. Virus seeds used by the vaccine are respectively a chicken infectious bronchitis virus Beijing A2 strain and a chicken new castle disease virus GM strain. The vaccine is prepared by the steps of mixing the chicken infectious bronchitis virus Beijing A2 strain and the chicken new castle disease virus GM strain after respectively being inactivated by an alkylating agent, adding oil adjuvant and emulsifying. The bigeminal inactivated vaccine can play a good role in preventing the novel serological type virus of infectious bronchitis and the novel antigen virus of new castle disease and solve the problem that the two viruses are only partially protected or even not protected by the prior vaccine in many places in China, thereby the occurrence of the chicken infectious bronchitis and the chicken new castle disease is effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biological medicines, and relates to a double inactivated vaccine of chicken infectious bronchitis and chicken Newcastle disease and a preparation method thereof. Background technique [0002] Chicken infectious bronchitis is an acute and highly contagious infectious disease caused by chicken infectious bronchitis virus. Quality drops. In the early 1990s, a variant infectious bronchitis with respiratory symptoms, kidney lesions and muscle necrosis broke out in the UK, mainly harming broiler breeders and laying hens. The strain is called 793 / B or 4 / 91. [0003] Newcastle disease is a highly contagious disease caused by Newcastle disease virus, which can infect various poultry, mainly chickens and turkeys. The disease can occur throughout the year, with the highest incidence in autumn, winter and early spring each year, mainly affecting the nervous system, respiratory system and digestive s...

AI Technical Summary

Problems solved by technology

[0005] 1. There are many serotypes of infectious bronchitis virus, which brings great difficulties to immune prevention. A single serotype vaccine can only produce immunity to the same type of infectious bronchitis virus, but not to other types of infectious bronchitis virus. The virus can only provide partial protection or no protection at all. For example, the vaccines prepared by the H52, H120, and M41 strains currently in use cannot provide protection against the infectious bronchitis virus that is prevalent in many places;

[0006] 2. At present, I, II, III, La Sota strain, N79 strain, N88 strain and other Newcastle disease vaccines are commonly used for Newcastle disease prevention in my country. Before the 1990s, when Newcastle disease occurred, vaccines such as LaSota strain and I strain were used for emergency Prevention works well, but in recent years the strains of Newcastle disease virus that are prevalent in many places have changed and the above vaccines no longer offer good protection