Single-cell micromanipulation device for microinjection

A technology of microinjection and micromanipulation, which is applied in the field of microsystems, can solve the problems of inability to meet the application requirements of cell microinjection, the inability to accurately adjust the cell posture, and the long training period of operators, so as to improve the degree of automation and experimental efficiency, Improve the success rate of the experiment and the effect of long service life

Inactive Publication Date: 2011-12-07
NANJING UNIV OF SCI & TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing contact mechanical operation method has the following disadvantages: it is difficult to adjust the position and posture of cells, it is seriously affected by the operator's subjective factors, the training period for operators is long, the cost is high, the automation is poor, and the cell posture cannot be adjusted accurately. The efficiency of microscopic experiments is low, it is easy to damage the manipulated cells, and the success of experiments is low
Therefore, the non-contact cell micromanipulation method cannot meet the application requirements of cell microinjection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single-cell micromanipulation device for microinjection
  • Single-cell micromanipulation device for microinjection
  • Single-cell micromanipulation device for microinjection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0018] Such as image 3 , during the cell operation process, the left and right end effectors 13, 3 form a stable single-cell micro-operation flow field with controllable velocity distribution on the inverted microscope workbench 10 to adjust the posture of the operated cell. The left and right end effectors 13, 3 and the o of the operated cell 14 c It is distributed in the center of symmetry. Under the condition of equal pressure applied by the gas source 1 and the pressure control valve 2, the internal cell culture fluid flows out of the port at the same speed, and forms an o c Centered vortex flow field. Under the action of the viscous drag force of the culture medium, the manipulated cells 14 are subjected to a planar force couple, resulting in rotational movement in the horizontal plane. By setting parameters such as the pressure value and action time of the left and right end effectors 13, 3, the rotation angle of the operated cell 14 in the horizontal plane can be adj...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a single-cell micro-operation device for micro-injection. The air source and pressure control valve of the device form a sealed air path with the left and right end effectors through the channels in the holder respectively. The platform is symmetrically installed on the left and right sides of the inverted microscope workbench, and the left and right end effectors form a single-cell micro-operation flow field with controllable flow velocity distribution on the inverted microscope workbench; Pressure control valve and left and right three-dimensional micro-motion platform. The invention effectively controls the flow field of the operating environment of the cell, forms a stable single-cell micro-operation flow field with controllable flow velocity distribution, drives the cell movement, realizes arbitrary adjustment of the cell angle in the three-dimensional orthogonal plane, and accurately controls the three-degree-of-freedom attitude of the cell ; Adjust the three-dimensional space position of the cell to realize the precise positioning of the cell position and posture.

Description

technical field [0001] The invention belongs to microsystem technology, and relates to a single-cell micro-operation device based on the coupling effect of mechanical motion and flow field flow, in particular to a single-cell micro-operation device for microinjection. Background technique [0002] In different cell engineering applications, the target of microinjection on cells is different, such as female pronucleus injected by pronucleus of zygote, polar body reorganized by polar body of oocyte, spindle of single sperm microinjected, etc. Precisely locate the position and posture of the manipulated cells. The existing single-cell micromanipulation methods are divided into two types: contact and non-contact, namely: [0003] 1. Contact type, mainly based on the mechanical operation mode of the existing manual adjustment microinjection system, such as the NT-88NE microinjection system of Japan Narishige Company. The end effector used for cell manipulation in this system ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36
Inventor 章维一侯丽雅田桂中
Owner NANJING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap