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A method for culturing tomato pollen temperature sensitive dolichostylous male sterile line

A technology of male sterile lines and male sterility, applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve problems such as unstable fertility and affecting seed quality, and achieve the effect of improving purity

Inactive Publication Date: 2009-08-26
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because its fertility is affected by the irregularity of the climate, it shows that the fertility is unstable and affects the quality of the seeds.

Method used

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  • A method for culturing tomato pollen temperature sensitive dolichostylous male sterile line
  • A method for culturing tomato pollen temperature sensitive dolichostylous male sterile line

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Embodiment Construction

[0010] In the present invention, the applicant recombines the long-style sterility gene and the tomato pollen temperature-sensitive sterility gene, and screens for the recombination of the long-style sterility gene and the tomato pollen temperature-sensitive sterility gene. At the same time, a new tomato pollen temperature-sensitive long-style male sterile line is selected to provide hybrid combination breeding.

[0011] The tomato pollen temperature-sensitive long-style male sterile line of the present invention is transformed according to the following specific operation steps:

[0012] 1. Materials: ① Female parent: The tomato pollen temperature-sensitive male sterile line (T-4) produced by the Department of Agriculture, Okayama University, Japan, which was induced by gamma ray irradiation was used as the female parent. Patent literature-"(j.japan.Soc.Hort.Sci.) 68(3): 566-568.1999" has been disclosed. ②Male parent: the long-style natural mutant of Marutama, which has exce...

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Abstract

The invention relates to a method for culturing tomato pollen temperature sensitive dolichostylous male sterile line, comprising performing artificial hybridization by adopting T-4 carrying with tomato pollen temperature sensitive recessive sterile gene as a mother plant and adopting mutant of Wanyu carrying with the dolichostylous gene, and then selecting the tomato pollen temperature sensitive dolichostylous male sterile line with stable sterility via eight genaration system in a pedigree method. Accordingly, the male sterility can not be influenced by the temperature, the sterile rate can be over 99%, and the hybrid purity is improved; in addition, self-reproduction of the sterile line can be carried out in autumn, and double hybrid tomato seed can be produced in spring.

Description

technical field [0001] The invention belongs to the field of cross-breeding of crops, in particular to a method for transferring tomato pollen temperature-sensitive long-style male sterile lines and its application. Background technique [0002] At present, when tomato hybridization is used domestically, artificial emasculation is generally adopted. This method is time-consuming, labor-intensive, inefficient, and expensive. If male sterile lines are used, seed production can not only save labor and time, but also ensure the quality of seeds. Important guarantee. At present, the utilization rate of tomato male sterility in domestic research is only 50% due to separation, and it can only be used after identification and confirmation of male sterile plants. Moreover, it is difficult to identify sterile plants and fertile plants, and it is difficult to be used in production. [0003] The tomato sterile line (T-4) produced by the Faculty of Agriculture, Okayama University, Japan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 王先裕黄元姣
Owner GUANGXI UNIV