Method for preparing bovine serum albumin hybridized membrane

A technology of bovine serum albumin and hybrid membrane, which is applied in the field of bio-inorganic hybridization, can solve the problem that the polypeptide chain has no biological activity, etc., and achieve the effects of environmentally friendly preparation method, compact membrane surface structure and simple preparation method

Inactive Publication Date: 2009-09-16
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although protein molecules are composed of polypeptide chains of amino acids connected end to end, a stretched or randomly arranged polypeptide chain is not biologically active

Method used

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  • Method for preparing bovine serum albumin hybridized membrane
  • Method for preparing bovine serum albumin hybridized membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] a. 3.7513g of Al (NO 3 ) 3 ·9H 2 O and 5.1280 g of magnesium nitrate (Mg(NO 3 ) 2 ·6H 2 O) was dissolved in 60 mL of deionized water. Slowly add a certain concentration of NaOH solution dropwise to excess lactic acid to adjust the pH value of the system to 6-11; add Mg / Al mixed salt solution, continue to add NaOH solution dropwise to pH 6-11, heat to reflux and crystallize 7 -12 hours, white slurry was obtained, centrifuged, washed and dried. After the above-mentioned lactic acid intercalated hydrotalcite is washed to neutrality, it is dispersed in a certain volume of water, and heated to reflux until peeling;

[0032] b. Under the condition of pH 5.0, prepare 30ml of bovine serum albumin solution with a concentration of 3mg / ml, add 10ml of hydrotalcite nanosheet solution, and assemble for 1h;

[0033] c. Immerse the treated substrate in the above-mentioned assembly system for 20 minutes, take it out, wash with water, and dry to obtain a powder-deposited hybrid f...

Embodiment 2

[0035] a. 3.7513g of Al (NO 3 ) 3 ·9H 2 O and 5.1280 g of magnesium nitrate (Mg(NO 3 ) 2 ·6H 2 O) was dissolved in 60 mL of deionized water. Slowly add a certain concentration of NaOH solution dropwise to excess lactic acid to adjust the pH value of the system to 6-11; add Mg / Al mixed salt solution, continue to add NaOH solution dropwise to pH 6-11, heat to reflux and crystallize 7 -12 hours, white slurry was obtained, centrifuged, washed and dried. After the above-mentioned lactic acid intercalated hydrotalcite is washed to neutrality, it is dispersed in a certain volume of water, and heated to reflux until peeling;

[0036] b. Under the condition of pH 8.0, prepare 30ml of 1mg / ml bovine serum albumin solution, add 10ml of hydrotalcite nanosheet solution, and assemble for 1h;

[0037] c. Immerse the treated substrate in the above-mentioned assembly system for 20 minutes, take it out, wash with water, and dry to obtain a powder-deposited hybrid film.

Embodiment 3

[0039] a. 3.7513g of Al (NO 3 ) 3 ·9H 2 O and 5.1280 g of magnesium nitrate (Mg(NO 3 ) 2 ·6H 2 O) was dissolved in 60 mL of deionized water. Slowly add a certain concentration of NaOH solution dropwise to excess lactic acid to adjust the pH value of the system to 6-11; add Mg / Al mixed salt solution, continue to add NaOH solution dropwise to pH 6-11, heat to reflux and crystallize 7 -12 hours, white slurry was obtained, centrifuged, washed and dried. After the above-mentioned lactic acid intercalated hydrotalcite is washed to neutrality, it is dispersed in a certain volume of water, and heated to reflux until peeling;

[0040] b. Under the condition of pH 5.0, prepare a bovine serum albumin solution with a concentration of 3 mg / ml;

[0041] c. The treated substrate was dipped into 50ml of hydrotalcite nanosheets for 20 minutes, washed with water and dried;

[0042] d. The above-mentioned substrate pre-spreaded with a layer of hydrotalcite film was dipped into the bovine...

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Abstract

The invention relates to a method for preparing bovine serum albumin hybridization membrane, belonging to the technical field of biological-organic hybridization. The preparation technique comprises preparation of powder hybridization membrane and layer-by-layer assembled hybridization membrane. In preparing layer-by-layer assembled hybridization membrane consisting of multilayer packaging protein and houghite nanoplates, the protein and the houghite form membrane in an alternating way in the layer-by-layer assembled hybridization membrane; characteristic spectrum intensity of the protein increases in a linear way along with the increase of assembled layers; the houghite plates are arranged on the surface of the substrate in a compact way, membrane structure of ovine serum albumin hybridization membrane prepared by layer-by layer assembly is even and continuous, the spectral intensity of the ovine serum albumin increases in a linear way along with the increase of assembled layers; the surface of the hybridization membrane is compact and smooth, the protein is evenly distributed on the surface of the membrane. The method has the advantages that the method for simultaneous preparation is easy in that no complex device is needed, the preparation cost is low, water is the only solvent in the preparation process and the preparation method is environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of biological-inorganic hybridization, and in particular relates to a method for preparing a bovine serum albumin hybrid film, and prepares a binary hybrid film of protein and hydrotalcite nanosheets. The surface of the hybrid film is smooth , with a uniform and continuous structure. Background technique [0002] Bio-inorganic hybrid technology, as an important branch of supramolecular chemistry, has always been the focus of scientific research. The construction and functionalization of bio-inorganic hybrid films is a research hotspot recently and for a long time in the future. Combining protein With the characteristics of wide application potential, the functionalization and deviceization of proteins can be realized through bio-inorganic hybrid technology, so as to realize the value of biomolecules in practical applications. Active biomolecules (such as enzymes, amino acids, and peptides) have broad applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L89/00C08K3/34B32B9/00
Inventor 何静周良彪
Owner BEIJING UNIV OF CHEM TECH
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