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Method for extracting medicinal large leaf moss polysaccharide

An extraction method and technology of grass polysaccharide, which are applied in the field of extraction and purification of grass polysaccharide, can solve the problems of less grass and no extraction of grass polysaccharide, etc., and achieve good medicinal effect, significant economic and social benefits. , the effect of high purity

Inactive Publication Date: 2009-09-23
KUNMING UNIV OF SCI & TECH
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is a pity that at present there are few studies on Herba huixincao at home and abroad, and they are mainly studied from the perspectives of pharmacognosy and overall clinical pharmacology, and there are few research reports on other aspects. to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Grind the Herba huixin, pass it through 60 mesh, put it in an ultrasonic extractor, add 20 times of water, adjust the pH to 8-9, raise the temperature to 90°C, extract for different times, combine the extracts, filter, and centrifuge for 15 minutes Then take the clear liquid; concentrate the extract under reduced pressure, remove the protein by savage method, then add edible ethanol to make the concentration reach 80%, and the resulting precipitate is refined, vacuum-dried, and pulverized to obtain the refined polysaccharide of Rhizoma chinensis. The extraction rate is shown in Table 1: it can be seen that the extraction time of 2 hours can meet the requirements.

[0019] Table 1 The polysaccharide yield of different extraction time

[0020] time / h

Embodiment 2

[0022] Grind the grass, pass through 60 meshes, put it in an ultrasonic extractor, add different multiples of water, adjust the pH to 8-9, raise the temperature to 90°C, extract for 2 hours, combine the extracts, filter, and centrifuge for 15 minutes Then take the clear liquid; concentrate the extract under reduced pressure, remove the protein by savage method, then add edible ethanol to make the concentration reach 80%, and the resulting precipitate is refined, vacuum-dried, and pulverized to obtain the refined polysaccharide of Rhizoma chinensis. The extraction rate is shown in Table 2: It can be seen that the solid-liquid ratio is 1:20-30. Considering that the subsequent process steps will be concentrated and the cost will be increased, it is more economical to choose 1:20.

[0023] Table 2 The yields of extracting polysaccharides from Coryne chinensis with different solid-liquid ratios

[0024] time / h

Embodiment 3

[0026] Grind the grass, pass it through 60 meshes, place it in an ultrasonic extractor, add 20 times of water, adjust the pH to 8-9, extract at different temperatures for 2 hours, and combine the extracts. After centrifuging for 15 minutes, take the supernatant; concentrate the extract under reduced pressure, remove the protein by the savage method, then add edible ethanol to make the concentration reach 80%, and the resulting precipitate is refined, dried in vacuum, and pulverized to obtain the fine polysaccharide of Rhizoma chinensis. The extraction rate is shown in Table 3: it can be seen that the yield is the highest when the extraction temperature is 90°C.

[0027] Table 3 Yields of polysaccharides from Grass japonicus at different temperatures

[0028] temperature / ℃

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Abstract

The invention belongs to a method for extracting active ingredients of traditional Chinese medicine, in particular to the method of extracting and purifying large leaf moss polysaccharide from large leaf moss. The method comprises the following steps of smashing the large leaf moss and using hot water to extract crude polysaccharide from the medicinal material in an ultrasonic extractor, centrifugation, filtration, concentration and protein removal are carried out on extract liquid, ethanol is further used for precipitation in a water extract to obtain the water soluble large leaf moss crude polysaccharide, then further separation and purification are carried out on the crude polysaccharide, and a purified large leaf moss polysaccharide fine product is obtained by vacuum drying. The method can extract the large leaf moss polysaccharide from the large leaf moss to the maximum extent and is safe, non-toxic, reliable, easy to operate and high in yield. The method provides possibility for playing the efficacy of the large leaf moss polysaccharide and preparing drugs or health care foods with a variety of indications.

Description

technical field [0001] The invention belongs to a method for extracting active components of traditional Chinese medicine, and more specifically relates to a method for extracting and purifying polysaccharides from Herba huixincao. Background technique [0002] At present, nearly a hundred kinds of polysaccharide compounds have been extracted. Through pharmacological research and clinical application, it has been proved that polysaccharides have the functions of anti-tumor, lowering blood fat, anti-thrombosis, enhancing immunity, and delaying aging. Therefore, more and more research and development of polysaccharides being valued by people. Polysaccharides are natural macromolecular compounds that exist in animals, plants and microorganisms. Polysaccharides are generally extracted from natural products. Natural polysaccharides are a class of important biologically active substances with various biological activities and health functions. They can be used as effective drugs a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张惠芬李宝才张水花戴伟峰
Owner KUNMING UNIV OF SCI & TECH
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