Unlock instant, AI-driven research and patent intelligence for your innovation.
Method for detecting microalbumin
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for microalbumin and albumin, applied in the field of microalbumin detection, can solve problems such as poor sensitivity, inability to detect, and inability to achieve accurate detection of nanogram-level albumin, and achieves the effect of reducing dosage and reducing pollution
Inactive Publication Date: 2009-11-18
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
View PDF0 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
This method has the advantages of good stability, high accuracy and specificity, but its sensitivity is poor, and only microgram level albumin can be detected. For albumin with lower content, such as nanogram level synthesized by hepatocytes in vitroTrace amounts of human albumin cannot be detected
Moreover, the inventors found that even if the concentration of the standard solution is lowered to the nanogram level to make a standard curve, the accurate detection of albumin at the nanogram level cannot be achieved
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment approach
[0035] An embodiment of implementing the method of the present invention is provided below, through which nanogram level albumin can be detected, the method comprises the following steps:
[0036] (1) Dilute the albumin standard with distilled water to 2ng / ml, 10ng / ml, 20ng / ml, 50ng / ml, 100ng / ml and 200ng / ml, take 200μl each, and record it as standard tube B 1 , B 2 , B 3 , B 4 , B 5 and B 6 ;
[0037] (2) Set the non-specific tube and zero standard tube, denoted as NSB and B 0 , add 300 μl and 100 μl of incubation solution (composition is PBS buffer) respectively;
[0038] (3) Take 200 μl of each sample to be tested and record it as sample tube S 1 ~S N , where N=1;
[0039] (4) Dilute the albumin antibody with 3 times the volume of distilled water, in B 0 , B 1 ~B 6 and S 1 ~S N Add 100 μl to each tube, shake well, and incubate at 37°C or 38°C for 4-6 hours;
[0040] (5) Take a blank tube and mark it as T; in T, NSB, B 0 , B 1 ~B 6 and S 1 ~S N Add to each ...
[0048] Purchase the albumin radioimmunoassay kit from Beijing Atomic High-Tech Co., Ltd., take 3 bottles of albumin standard (lyophilized product) and add distilled water to fully dissolve it, and make the concentration of 1μg / ml, 2μg / ml and 5μg / ml Albumin standard substance; the albumin standard substance in the sample to be tested and the albumin radioimmunoassay kit, 125 I-albumin, incubation solution, albumin antibody, separation reagent shake well and equilibrate to room temperature.
[0049] 2. Drawing of standard curve and detection of samples
[0050] 1. Dilution of albumin standard: Take 7 clean test tubes and mark them as A, B, C, D, E, F and G respectively, accurately measure 100 μl of 1 μg / ml albumin standard into tube A, and then Measure 900 μl of distilled water into tube A, shake well, and prepare 100 ng / ml albumin standard; accurately measure 100 μl of 100 ng / ml albumin standard from tube A, add it into tube B, and then measure...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention provides a method for detecting albumin with nanogram content, which comprises the following steps: diluting an albuminstandard product into concentration gradient which is properly distributed in nanogram level, adding excessive albuminantibody, and performing first incubation; adding excessive albumin labeled with radioactive isotope relative to residual albumin antibody into liquid after the incubation, and performing secondary incubation to combine partial albumin labeled with the radioactive isotope and the albumin antibody into a compound; separating the compound and determining radiocounting of the compound; determining a relational expression between the radiocounting and concentration according to the radiocounting and the concentration of the albumin in a corresponding standard product; and repeating the steps by replacing the albumin standard product by a sample to be tested, and determining the concentration of the albumin in the sample to be tested according to the measured radiocounting of the sample to be tested and the relational expression. The method can detect the albumin with the nanogram content, and has high accuracy and good stability.
Description
technical field [0001] The invention relates to a method for detecting microalbumin, in particular to a method for detecting albumin whose content is nanogram level. Background technique [0002] Albuminradioimmunoassay is a radioimmunoassay technique used to determine the content of albumin in human urine or body fluids. Its principle is: the albumin in the sample to be tested and the standard 125 I-albumin competes for binding with a limited amount of antibody, 125 A part of I-albumin is combined with the antibody to form a complex, and the other part is free; the complex part is separated from the free part by centrifugation, and the precipitate is taken and its radioactive count is determined; The radioactive intensity is negatively correlated. Using a series of albumin with different concentrations as a standard, a standard curve can be obtained, and the albumin content in the sample can be found from the standard curve. The albumin radioimmunoassay kit currently on ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More