Enzymoimmunoassay of beta-lactam medicine residual quantity in royal jelly

A technology of lactams and ELISA, which is applied in the field of detection of drug residues in royal jelly, can solve the problems of increased difficulty and instability, antibody difficulties, interfering substances, etc., and achieves reduced instrument analysis costs, simple equipment requirements, and satisfactory Determining the effect of requirements

Inactive Publication Date: 2009-11-25
THE INSPECTION & QUARANTINE TECH CENT ZHEJIANG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is because on the one hand, royal jelly contains a variety of active ingredients, complex composition, and a large number of interfering substances, which increases the difficulty and instability in specific operations; on the other hand, β-lactam drugs are easily Degradation, it is very difficult to prepare corresponding antibodies

Method used

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  • Enzymoimmunoassay of beta-lactam medicine residual quantity in royal jelly
  • Enzymoimmunoassay of beta-lactam medicine residual quantity in royal jelly
  • Enzymoimmunoassay of beta-lactam medicine residual quantity in royal jelly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] Embodiment: (the ELISA assay is carried out to the extract with the enzyme-linked immunoassay kit that British RANDOX company produces)

[0018] 1) Cross-reaction test

[0019] Add different concentrations of β-lactam drugs to the dilution buffer included in the detection kit to conduct a selectivity test (results are shown in Table 1), and the cross-reactivity rate of different substances is calculated by the following formula:

[0020] Cross-reactivity rate (%) = I 50 (Ampicillin) / I 50 (to be tested)×100

[0021] I 50 It is the concentration of β-lactam drug or analyte that produces 50% maximum inhibition rate in the reaction.

[0022] The crossover data of different β-lactam drugs are shown in Table 1:

[0023]

[0024] From the cross-reaction results, it can be seen that the kit has good cross-reactivity to nafcillin, ampicillin, amoxicillin, piperacillin, azlocillin, cloxacillin, penicillin G, dicloxacillin and oxacillin. The cross-reactivity between mayta...

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Abstract

The invention relates to an enzymoimmunoassay of beta-lactam medicine residual quantity in royal jelly. At present, no screening method of the beta-lactam residual quantity in royal jelly sample exists. The invention comprises the steps of 1) adding methanol aqueous solution in a royal jelly sample in a volume ratio of 48-52% and adequately and uniformly mixing; 2) centrifuging at a high speed at 15(+ / -)2 DEG C until the mixture is clear; 3) adding a thinning washing buffer solution in the supernatant; 4) adjusting the pH value to be 6.5-7.5 by an alkali or an acid; and 5) detecting through a kit for enzymoimmunoassay, wherein the thinning washing buffer solution is included in a detection kit or a phosphate buffer solution, and the pH value thereof is 7.0. The invention realizes the quick extraction and measurement of the beta-lactam medicine residual quantity, has a determination lower limit reaching 10 microgram / kg, and satisfies the determination requirements on the beta-lactam residual quantity in royal jelly product at home and abroad.

Description

technical field [0001] The invention relates to a detection method for drug residues in royal jelly, in particular to a method for detecting nafcillin, ampicillin, amoxicillin, piperacillin, azlocillin, cloxacillin, penicillin G, dicloxacillin and oxacillin in royal jelly Rapid extraction and enzyme-linked immunoassay method for total residues of nine β-lactam drugs. Background technique [0002] β-lactams (Beta-Lactams) refer to a large class of antibiotics with a β-lactam ring in their chemical structure, including the most commonly used clinical penicillins and cephalosporins, as well as the newly developed cephamycins and thiamycins , monocyclic β-lactams and other atypical β-lactam antibiotics. The antibacterial mechanism of β-lactams is similar, and they can all inhibit cell wall mucopeptide synthase, that is, penicillin-binding protein, thereby hindering the synthesis of cell wall mucopeptides, causing bacterial cell wall defects, and bacterial cell swelling and crac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/31C07D499/74C07D499/68C07D499/76C07D499/46
Inventor 张晓峰陈笑梅施伟良朱振江
Owner THE INSPECTION & QUARANTINE TECH CENT ZHEJIANG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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