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Application of small interfering RNA in preparing medicament for treating colon cancer

A small interference, colorectal cancer technology, applied in the field of biomedicine, can solve the problems of poor prognosis of patients with curative effect, achieve the effect of inhibiting proliferation activity, blocking growth cycle, and inducing apoptosis of colorectal cancer cells

Inactive Publication Date: 2009-12-23
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of application of small interfering RNA in the preparation of the medicine for treating colorectal cancer, described this small interfering RNA will solve the curative effect and patient prognosis of using chemotherapy to treat advanced colorectal cancer in the prior art Poor technical issues

Method used

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  • Application of small interfering RNA in preparing medicament for treating colon cancer
  • Application of small interfering RNA in preparing medicament for treating colon cancer
  • Application of small interfering RNA in preparing medicament for treating colon cancer

Examples

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Embodiment 1

[0027] Example 1 In vitro study

[0028] The experimental materials of this example are human colorectal cancer cell line HCT116 purchased from the Institute of Cell Biochemistry, Chinese Academy of Sciences, and McCOY'S5A medium purchased from Sigma Company of the United States; SiRNA fragments targeting the four potential target sites of the mTOR gene and negative controls were synthesized by Dharmacon Company of the United States (Pre-designed RNAi Reagents, Dharmacon, USA); transfection reagent: DharmaFECT TM 4siRNA Transfection Reagent (T-2004-03); 5×SiRNA Buffer: Dharmacon, USA; TRIzol Reagent: Invitrogen; MTT, Triton X-100, propidium iodide (PI): Sigma; RNase A: Roche Company; See Table 1 for the sequences of siRNA, primers, and probes; see Table 2 for the antibodies used in the test; kit for chemiluminescent detection: Super West Femto Maximum Sensitivity Substrate(34095); Super West Pico Chemilu-minescent Substrate (34077), both products of Pierce Company; cell c...

Embodiment 2

[0106] Example 2 In vivo studies

[0107] Experimental materials in this example Experimental animals: BALB / c nude mice, male, 4 weeks old, inbred strain, purchased from the Songjiang Animal Experiment Base of the Chinese Academy of Sciences (SCXK (Shanghai) 2003-0003), bred at the IVC of Shanghai Jiao Tong University School of Medicine Animal laboratory (SYXK (Shanghai) 2003-0007); siRNA: using the siRNA3 and negative control (NegativeControl) sequences shown in Table 1, modified with methoxy by Shanghai GenePharma Co., Ltd, and chemically synthesized ; Transfection reagent: in vivo-jetPEI TM (201-50G, Polyplus Transfection, Illkirch, France); Antibodies used in the test are shown in Table 2; TUNEL Apoptosis Detection Kit: Roche, 1684809a; 50 μl micro-syringe: Shanghai Tiancheng Technology Co., Ltd.; Vernier caliper: Shanghai Precision Instruments Co., Ltd.; ultrasonic cell pulverizer (JY92-II): Ningbo Xinzhi Biotechnology Co., Ltd.; paraffin slicer (RM21459), embedding mach...

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Abstract

The invention provides application of a small interfering RNA in preparing a medicament for treating colon cancer, wherein the small interfering RNA can inhibit the proliferation activity of colon cancer cells, retard the growth cycle of the colon cancer cells, induce the apoptosis of the colon cancer cells, and lower the activity of an mTOR / p70S6K / 4EBP1 signal channel of the colon cancer; besides, the small interfering RNA has therapeutic action on the transplantable tumor of nude mice colon cancer, and solves the technical problem that the prior art has poor curative effect and patient prognosis by adopting chemotherapy to treat middle and advanced stage colon cancer.

Description

Technical field: [0001] The invention relates to the field of biomedicine, in particular to a drug for treating tumors, in particular to the application of a small interfering RNA in the preparation of a drug for treating colorectal cancer. Background technique: [0002] With the improvement of people's living standards and changes in diet structure, the incidence of colorectal cancer in my country is on the rise. Therefore, exploring effective strategies for the prevention and treatment of colorectal cancer is an important issue related to national health. However, since the mechanism of the occurrence and development of colorectal cancer is still unclear, there is a lack of effective prevention and treatment targets and means. Especially for middle-advanced colorectal cancer, the treatment method can only be chemotherapy, and the curative effect and prognosis of patients are poor. [0003] Mammalian target of rapamycin (mTOR)-mediated signaling pathway is one of the most ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105A61K48/00A61P35/00
Inventor 房静远张燕捷
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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