Evaluation Method of Combined Effect in Infrared Test of Acute Toxicity Algae

Abstract

The evaluation method of joint action in the acute toxicity algae infrared test is characterized in that, constructs joint deviation coefficient method (joint deviation coefficient is represented by θi) with joint action coefficient θ and total average deviation coefficient f, adjusts the value of additive action with f=0.06 Theoretical value of θ (θ=1). Evaluate according to the change of joint deviation coefficient θi, and the evaluation method is as follows: θi>1+f, that is, θi>1.06 is synergistic effect; 1-f≤θi≤1+f, that is, 0.94≤θi≤1.06 is additive effect; θi<1-f, that is, θi<0.94 is antagonism; Tφ<Tβ<Tλ-f Tλ is an independent effect (Tφ: the temperature difference produced by the drug with the strongest toxic effect, Tβ-the temperature difference of the combined effect, Tλ-the additive effect The temperature difference, f·Tλ-deviation temperature difference). The test method of the present invention is simple and convenient; the action of the binary drug on sensitive algae shows four types of joint action: synergy, addition, antagonism and independence; Cell number, chlorophyll content, and dry cell weight are used as test indicators. The experimental period of the toxicity test is 24h to 96h.

Description

technical field [0001] The invention relates to an evaluation method for the joint action in the infrared test of acute toxicity algae, and belongs to the technical field of ecotoxicology. Background technique [0002] The comprehensive biological effect produced by the joint action of two or more chemical pollutants is called joint action. In the environment, multiple pollutants often exist at the same time, and the combined effects of multiple and single chemical pollutants on organisms are completely different. Therefore, one of the research trends in ecotoxicology is to strengthen the comprehensive effects of pollution and the environmental effects of multi-component composite pollution. (Kong Fanxiang. 2000. "Environmental Biology" [M]. Beijing: Higher Education Press: 13, 92-93). However, the study of these two effects requires the evaluation method of joint action and the establishment of the type of joint action of toxicants, there is no standard method (Kong Fanxia...

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Problems solved by technology

At present, the acute toxicity of heavy metals in the environment has been tested by algae infrared radiation (Guo Weihua, Su Haiyan, Zhang Zhi, Wang Bin, You Xiantong. 2008a. The acute toxicity of heavy metals in the environment by algae infrared radiation test [J]. Ecological Environment, 17(2): 520- 523), Acute Toxicity of Pesticide Residues in Algae Infrared Test (Guo Weihua, Wang Xiang, Zhang Zhi, You Xiantong, Wang Bin. 2008b. Study on Acute Toxicity of Pesticide Residues in Algae Infrared Test Environment [J]. Journal of Agricultural Environmental Science, 27(5): 2039-2042), the determination method of sensitive algae in the infrared test of acute toxic algae (Guo Weihua, Ding Yanyan, Zhang Zhi, Wang Xiang, Su Haiyan. 2008c. The determination method of sensitive algae in the infrared test of acute toxic algae[J]. Ecotoxicology Chinese Journal, 3(6): 577-583), however, there is no report on the joint effect of algae infrared test method at home and abroad.

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