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Method for detecting serum marker of pancreatic cancer

A serum marker and pancreatic cancer technology, applied in the field of medical molecular biology, can solve the problems of losing surgical opportunities and achieve great clinical application value, good sensitivity, and high accuracy

Inactive Publication Date: 2009-12-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, the vast majority of pancreatic cancer patients are diagnosed at an advanced stage (TNM stage III, IV) and lose the opportunity for surgery

Method used

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  • Method for detecting serum marker of pancreatic cancer
  • Method for detecting serum marker of pancreatic cancer
  • Method for detecting serum marker of pancreatic cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Serum collection:

[0036] Take 31 blood samples from patients with pancreatic ductal adenocarcinoma confirmed by surgery in Changhai Hospital, let each whole blood sample stand at room temperature (30 minutes to 2 hours), centrifuge at 1200×g for 20 minutes at 4°C, and take the upper layer of each sample 500ul of serum was transferred to a 1.5ml centrifuge tube and stored in a -80°C refrigerator.

[0037] 2. RNA extraction from serum:

[0038] Take 50 μl of each serum sample, add 750 μl TRI REAGENT BD (Molecular Research Center Company) and 20 μl 5N acetic acid, mix well and add 5 μl of Cel-miR-39 (artificially synthesized microRNA, provided by Qiagen Company, with a concentration of 50 pmol / L) Remove the detection difference caused by human operation), shake and mix, let stand at room temperature for 5 minutes, add 200 μl of chloroform, shake and mix, let stand at room temperature for 5 minutes, centrifuge at 12000×g for 15 minutes at 4°C, extract the supernatant,...

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Abstract

The invention relates to the technical field of medical molecular biology and provides a method for detecting the serum marker of pancreatic cancer. The pancreatic cancer has high grade malignancy, difficult early diagnosis and poor prognosis, and the pancreatic cancer also lacks really effective solution, with surgical resection as the only therapeutic method to prolong survival period. Unfortunately, most of pancreatic cancer patients are in the advanced stage (TNM belongs to III and IV stages) and miss the surgical option. The invention aims at providing a method for detecting the serum marker of pancreatic cancer and applying the method to early diagnosis of pancreatic cancer and clinical judgment of laparotomy indication. The study proves that in the invention, the expression level of miR-196a in the serum is closely related to the postoperative survival period of pancreatic cancer; the study later proves that the relative expression abundance of miR-196a can well distinguish resectable pancreatic cancer (TNM belongs to I and II stages) from pancreatic cancer in advanced stage (TNM belongs to III and IV stages), so the method in the invention can be used for detecting the serum marker microRNA-196a of pancreatic cancer, and the method further has the advantages of convenient detection, good sensitivity and high accuracy.

Description

technical field [0001] The invention relates to the technical field of medical molecular biology, and relates to a method for detecting serum markers of pancreatic cancer. The serum marker of pancreatic cancer is specifically microRNA-196a. Background technique [0002] MicroRNA, first discovered in 1993, is a small, non-protein-coding single-stranded RNA consisting of 18 to 25 nucleotides. MicroRNA achieves its biological function by binding to the 3'untranslated region (3'UTR) of mRNA, degrading the target mRNA or inhibiting its translation. After more than 20 years of hard work, scientists have made a breakthrough in the research of microRNA, and found that it is ubiquitous in animals, plants and viruses, and plays a very important role in many life activities. According to the latest microRNA database statistics (miRBase Registry 13.0), 706 microRNAs have been found in the human body. microRNA is highly conserved, time-sequential and tissue-specific. [0003] Recent s...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 李兆申孔祥毓荆清杜奕奇王国坤高军林寒
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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