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Pilatory containing Wnt protein

A hair growth agent and protein technology, applied in the direction of medical preparations containing active ingredients, peptide/protein components, drug combinations, etc., can solve problems such as unsatisfactory curative effect, unsatisfactory curative effect, and insignificant improvement of symptoms, etc., to achieve Quick onset, obvious effect, and clear mechanism

Inactive Publication Date: 2010-02-10
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficacy of most products on the market is not satisfactory, and the symptoms of patients do not improve significantly after use
Relevant surveys also show that about 98% of hair loss patients believe that traditional hair loss drugs on the market cannot bring satisfactory results, and most patients will choose to give up when trying hair growth agents for half a year.

Method used

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  • Pilatory containing Wnt protein
  • Pilatory containing Wnt protein
  • Pilatory containing Wnt protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1A

[0037] Construction and amplification of embodiment 1 AdWnt3a

[0038] Using the AdEasy system of He Tongchuan et al., the titer of AdWnt3a obtained was 10 9pfu / ml. Reference gene sequence NM_011718.1. (See literature for specific methods: 1. He TC, Zhou S, da Costa LT, et al. ASimplified system for generating recombinant adenoviruses. Proc Natl Acad Sci US A, 1998, 95: 2509-14 2. Luo J, Deng ZL, Luo X, et al. A protocol for rapid generation of recombinant adenoviruses using the AdEasy system. Nat Protoc, 2007, 2: 1236-47.)

Embodiment 2

[0039] Embodiment 2Wnt3a promotes hair growth test

[0040] (1) Animal experiments

[0041] 1) Inject 8-week-old female C57BL / 6 mice. At this time, the dorsal hair of the mice is in the stationary phase. The groups are as follows: 36 mice in the recombinant adenovirus AdWnt3a injection group, 36 mice in the AdGFP control group (empty vector), and 36 mice in the PBS control group. Only.

[0042] 100 μl of 1% pentobarbital was injected intraperitoneally. About 5 minutes later, the mice were under anesthesia, and the hair on the back of the mice was carefully shaved with a shaver, taking care not to shave the skin or pull out the hair follicles.

[0043] After shaving, take a point along the middle of the back midline, inject 25 μl AdWnt3a intradermally, and the virus titer is 10 8 pfu / ml, be careful not to inject subcutaneously. After the injection, a pit with a diameter of about 5 mm was produced at the injection site, and the injection site was marked along the edge of the...

Embodiment 3

[0067] Embodiment 3 result analysis

[0068] 1. Acquisition of AdWnt3a

[0069] After multiple amplifications, the titer of AdWnt3a was 10 9 pfu / ml. image 3 293 cells infected with AdWnt3a for 2 days. It can be seen that 2 days after infection, the infection rate of 293 cells reached 100%.

[0070] 2. Adwnt3a promotes hair growth

[0071] (1) Animal experiments. 9 days after AdWnt3a injection, the skin at the injection site began to turn black (appeared in 15 of 18 animals); 11 days, hair shafts grew out of the epidermis (appeared in 10 of 12 animals); 11 of them appeared). During the whole period, the injection site was still in the quiescent phase in the AdGFP injection group and the PBS injection group, and there was no significant change with the injection periphery. See Figure 4 , wherein, the left two are the PBS control group and the empty vector control group respectively, and the right three are the AdWnt3a injection group. It can be seen that a new coat (a...

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Abstract

The invention provides a pilatory containing Wnt protein. The pilatory contains Wnt protein which enhances the growth of hair follicle by a Wnt / Beta-catenin signal channel. The pilatory leads the hairfollicle to have a growing period phenotype, and the hair follicle is transformed to the growing period from a stationary period. The pilatory has the advantages of obvious effect, fast effect, clearmechanism and the like and has important social and economical significance to develop drugs for enhancing the growth of hair.

Description

technical field [0001] The present invention relates to a hair tonic, in particular to a hair tonic for promoting hair follicle growth through Wnt protein acting through Wnt / β-catenin signaling pathway, in particular to a hair tonic containing Wnt3a protein for promoting hair follicle growth. Background technique [0002] Hair and hair are unique to mammals. They are keratinized filamentous structures that grow on the surface of the skin all over the body, and are appendages derived from the epidermis. Animal skin has a lot of hair, which plays an important role in maintaining body temperature and resisting mechanical damage. Human hair has been quite degenerated, but the hair follicles are rich in nerve endings and are sensitive touch receptors. At the same time, human hair affects people's appearance and has important social significance. [0003] The hair protruding from the skin is called the hair shaft, and the hair buried in the skin is called the hair root. The hair...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P17/14
Inventor 杨恬李玉红连小华何通川
Owner ARMY MEDICAL UNIV
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