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Hollow polysaccharide microsphere immobilized enzyme and preparation method thereof

A technology for immobilizing enzymes and polysaccharides, which is applied in the direction of immobilization on or in biological cells, on/in organic carriers, etc., which can solve the problem that the structure is easily affected by the external environment, difficult to separate and recover, and difficult to continue Utilize and other problems to achieve the effect of simple technology, high specific surface area, and rich surface functional groups

Inactive Publication Date: 2011-04-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional free enzymes have some disadvantages, such as their structure is easily inactivated by the external environment; it is difficult to separate and recover after use, and it is difficult to achieve continuous utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, hollow polysaccharide microsphere immobilized enzyme

[0019] a) Preparation of hollow polysaccharide microspheres

[0020] Weigh 5 g of ellipsoidal beer yeast (Saccharomyces cerevisiae), disperse it in 100 ml of deionized water to make a yeast suspension, then transfer the yeast suspension to a reaction kettle, place it in a constant temperature box and heat it at 180°C for 18 hours. , the precipitate was collected, the obtained precipitate was washed with deionized water, and then dried at 20 °C for 24 h. After drying, hollow polysaccharide microspheres are obtained. Observing the hollow polysaccharide microspheres with a scanning electron microscope, the hollow polysaccharide microspheres are spherical with a diameter of 2-3 microns.

[0021] b) Preparation of hollow polysaccharide microsphere immobilized enzyme

[0022] Take step a) 5 g of spherical hollow polysaccharide microspheres, disperse them in 100 mL of 20% epichlorohydrin alkaline solution,...

Embodiment 2

[0026] Embodiment 2, hollow polysaccharide microsphere immobilized enzyme

[0027] a) Preparation of hollow polysaccharide microspheres

[0028] Weigh 5 g of ellipsoidal beer yeast (Saccharomyces cerevisiae), disperse it in 100 ml of deionized water to make a yeast suspension, then transfer the yeast suspension to a reaction kettle, place it in a constant temperature box and heat it at 180°C for 18 hours. , the precipitate was collected, the obtained precipitate was washed with deionized water, and then dried at 20 °C for 24 h. After drying, hollow polysaccharide microspheres are obtained. Observing the hollow polysaccharide microsphere with a scanning electron microscope, the hollow polysaccharide microsphere is a regular spherical shape with a diameter of 2-3 microns.

[0029] b) Preparation of hollow polysaccharide microspheres to immobilize enzymes

[0030] Take 5 g of the spherical hollow polysaccharide microspheres in step a), disperse them in 100 mL of an alkaline so...

Embodiment 3

[0033] Embodiment 3, hollow polysaccharide microsphere immobilized enzyme

[0034] a) Preparation of hollow polysaccharide microspheres

[0035] Weigh 5 g of Saccharomyces cerevisiae, saccharomyces cerevisiae, and disperse it in 100 ml of deionized water to make a yeast suspension, then add dilute hydrochloric acid to the yeast suspension to make the final concentration 0.1 mol / L, and mix the suspension The solution was transferred to a reaction kettle, placed in a thermostat and heated at 150°C for 24h. After the reaction, the precipitate was collected, washed with deionized water, and then dried at 20°C for 24h. After drying, hollow polysaccharide microspheres are obtained. Observing the hollow polysaccharide microspheres with a scanning electron microscope, the hollow polysaccharide microspheres are jar-shaped with a diameter of 2-3 microns.

[0036] b) Preparation of hollow polysaccharide microsphere immobilized enzyme

[0037] Take 5 g of the jar-shaped hollow polysacc...

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Abstract

The invention discloses a hollow polysaccharide microsphere immobilized enzyme and a preparation method thereof. The hollow polysaccharide microsphere immobilized enzyme comprises enzyme and a carrier, wherein the carrier is hollow polysaccharide microsphere; and the preparation method of the hollow polysaccharide microsphere comprises the steps of carbonizing yeast in liquid at a constant temperature and obtaining the hollow polysaccharide microsphere, wherein the constant temperature is any temperature from 150 to 240 DEG C. The invention also discloses a preparation method for the hollow polysaccharide microsphere immobilized enzyme. The hollow polysaccharide microsphere immobilized enzyme prepared by the method has a wide raw material source for preparing the carrier, and is simple and easy in technology of carrier preparation, surface modification and enzyme immobilization; in addition, the carrier is characterized by high specific surface area, large inner space, rich surface functional groups, good biocompatibility and the like.

Description

technical field [0001] The invention relates to a hollow polysaccharide microsphere immobilized enzyme and a preparation method thereof. Background technique [0002] Enzyme is a biocatalyst with important application value in many fields. It has the advantages of high catalytic rate, mild catalytic conditions, low energy consumption and no pollution. Traditional free enzymes have some disadvantages, such as their structure is easily inactivated by the external environment; it is difficult to separate and recover after use, and it is difficult to realize continuous utilization. Enzyme immobilization technology can effectively solve these problems, not only retaining the catalytic properties of the enzyme, improving the storage stability and operation stability of the enzyme, but also realizing the recovery and continuous utilization of the enzyme, thus reducing the production of enzyme preparations. The input cost and simplifies the subsequent product purification process. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/16C12N11/04C12N11/02
Inventor 杨森周克斌倪德志王雷
Owner CHINA AGRI UNIV
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