Separation method of rhubarb-combined anthraquinone and rhubarb tannin

A separation method and a combined technology are applied in the directions of drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effect of reducing tannin content and high utilization value

Inactive Publication Date: 2011-03-23
TIANJIN NANKAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the removal of tannin is a difficult problem in traditional Chinese medicine preparations. Some methods for removing tannin remove a large amount of active ingredients while removing tannin, and some methods will also introduce other impurities.

Method used

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  • Separation method of rhubarb-combined anthraquinone and rhubarb tannin
  • Separation method of rhubarb-combined anthraquinone and rhubarb tannin
  • Separation method of rhubarb-combined anthraquinone and rhubarb tannin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) 1kg of Chinese medicinal material (root or rhizome) of selected and washed rhubarb, add 15kg of boiling water, decoct for 30 minutes, filter with 80 mesh sieve, and collect the liquid; cut the rhubarb into slices of 0.5mm-2mm, Add 10kg boiling water to extract 3 times, 20 minutes each time, combine all the liquid medicines, add 222g of soy protein powder, let stand for 24 hours, filter with 80 mesh sieve, and dry the precipitate at low temperature to obtain rhubarb tannic acid protein extract to realize rhubarb tanning Quality separation; the supernatant is passed through the processed macroporous resin, eluted with 70% ethanol, the eluate is collected, the ethanol is recovered, and dried to obtain the rhubarb-bound anthraquinone extract.

[0025] (2) Assay method: determination of rhubarb-bound anthraquinone content, refer to the method "Assay of bound anthraquinone" in invention patent application 200510094530.8 "Compound drug granules for the treatment of intestinal ...

Embodiment 2

[0047] 1kg of selected and washed Chinese medicinal materials (root or rhizome) of rhubarb, add 10kg of boiling water, decoct for 20 minutes, filter with 80 mesh, and collect the liquid; cut the rhubarb into slices of 0.5mm-2mm and add 15kg respectively Extract 4 times in boiling water, 15 minutes each time, combine all the medicinal solutions, add 125g of soy protein powder, stand for 24 hours, filter with 80 mesh sieve, and dry the precipitate at low temperature to obtain rhubarb tannic acid protein extract to realize rhubarb tannin separation; The supernatant is passed through the processed macroporous resin and eluted with 60% ethanol, the eluate is collected, the ethanol is recovered, and dried to obtain a rhubarb-bound anthraquinone extract.

Embodiment 3

[0049] Pick and wash the Chinese medicinal material Rhubarb (root or rhizome) 1kg, add 4kg of boiling water, decoct for 15 minutes, filter with 80 mesh sieve, and collect the liquid; cut the rhubarb into slices of 0.5mm-2mm and add 20kg respectively Extract 3 times in boiling water, 30 minutes each time, combine all the medicinal solutions, add 333g of soy protein powder, let stand for 24 hours, filter with an 80 mesh sieve, and dry the precipitate at low temperature to obtain the rhubarb tannin protein extract to realize the separation of rhubarb tannins; The supernatant is passed through the processed macroporous resin and eluted with 80% ethanol, the eluate is collected, the ethanol is recovered, and dried to obtain a rhubarb-bound anthraquinone extract.

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Abstract

The invention discloses a separation method of rhubarb-combined anthraquinone and rhubarb tannin, belonging to the separation of anthraquinone. The method comprises the steps: one part of traditional Chinese medicine rhubarb sub cargo is weighted according to the weight parts, and then added into 4-15 parts of boiling water for decoction, and the liquid medicine is collected; the rhubarb sub cargo is sliced, then extracted for 3-4 times by boiling water with 10-25 parts of water adding amount each time, and then filtered, and all the liquid medicine is merged; 0.1-0.5 part of soy protein flour is added into the liquid medicine, then the liquid medicine stands and is filtered by a screen with 60-80 meshes, and then the deposit is dried at low temperature to obtain the rhubarb tannalbin extractive, thus realizing the separation of rhubarb tannin; liquid supernatant is taken and passes through the well-treated macroporous resin, and then is eluted by 50%-90% ethanol, the eluent is collected, the ethanol is recovered, and the rhubarb-combined anthraquinone extractive is obtained after drying. Since the soy protein is a food component and has no poison, the separation method kills twobirds with one stone, and respectively obtains the rhubarb-combined anthraquinone extractive and the rhubarb tannalbin extractive.

Description

Technical field [0001] The invention relates to the separation of anthraquinones, in particular to a method for separating rhubarb-bound anthraquinones and rhubarb tannins. technical background: [0002] The dried roots and rhizomes of Rheum palmatum (Rheum palmatum L.), Rheum tanguticum Maxim.ex Balf. or Rheum officinale Baill. Detoxify blood, remove blood stasis and clear menstruation [0003] The main components of rhubarb are: anthraquinones, stilbene glycosides, chromones, naphthol glycosides, tannins, etc. The content of anthraquinones is 3%-5%, which is divided into bound anthraquinone and free anthracene Quinones, free anthraquinones include rhein, emodin, aloe-emodin, physcion, isoe modin, and chysophanol The combined anthraquinone mainly includes anthraquinone glycosides and bisanthraquinone ketone glycosides. The anthraquinone derivatives in rhubarb mainly have diarrhea effect, antibacterial effect, hemostasis and activating blood circulation, antipyretic effect, anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/708A61P1/10A61P31/04A61P7/04A61P29/00A61P11/00A61K125/00
Inventor 吴咸中伍孝先刘俊红李棣华赵连根卓玉珍
Owner TIANJIN NANKAI HOSPITAL
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