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Magnetic bacteria separometer

A magnetic bacteria and separation tube technology, which is applied in biochemical instruments, biochemical equipment and methods, and methods for stress-stimulated microbial growth, etc. The effect of the amount of suspension

Inactive Publication Date: 2012-03-07
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because all the static magnet blocks or simple electromagnetic coils are used, the magnetic field strength generated is limited, and the magnetic field distribution is not optimized, so the above three methods cannot efficiently separate magnetotactic bacteria.
Especially for some weak magnetotactic bacteria, the above three methods are completely unsuitable
Moreover, the processing capacity of the above method is limited, and continuous operation is not possible. The magnetotactic bacteria isolated each time must be activated and expanded, which affects the cultivation efficiency of the magnetotactic bacteria.

Method used

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0013] see figure 1 , the present invention includes an adjustment controller 1, a rectifier 2, two coils 3, two electromagnets 4, a separation tube 5, a sample pool 6, a constant current pump 7, a bracket 8, and a collection pool 9, and the two electromagnets 4 are relatively fixed On the bracket 8, the rectifier 2 is connected to the coil 3 of the electromagnet, the opposite surfaces of the two electromagnets 4 are two hyperboloids, the separation tube 5 is placed between the two magnetic pole surfaces of the electromagnet, and the inlet of the separation tube is connected to a constant current Pump 7 , the two outlets ( 10 , 11 ) of the separation tube receive sump 9 . The adjustment controller 1 controls the constant flow pump 7 .

[0014] The pole head surfaces of two electromagnets 4 are hyperbolic shapes among the present invention, and the mag...

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Abstract

The invention discloses a magnetic bacteria separometer which comprises two pieces of electromagnet, a separating tube and a constant flow pump system, wherein the two pieces of electromagnet are fixed on a bracket; a rectifier is connected with coils of the two pieces of electromagnet; the opposite surfaces of the two pieces of electromagnet are two double curved surfaces; and the separating tube is arranged between two magnetic pole surfaces of the electromagnet, an inlet of the separating tube is connected with the constant flow pump system, and two outlets thereof are connected with a collecting tank. The magnetic bacteria separometer can be used for separating the bacteria with different magnetotaxis in a liquid culture medium, has high efficiency, saves the time, is large in treatment capacity and continuous in operation, and can meet the separation requirement of various magnetotactic bacteria including weak magnetotactic bacteria.

Description

technical field [0001] The invention relates to a bacteria separating device, in particular to a magnetic bacteria separating instrument. technical background [0002] The magnetic separation of magnetotactic bacteria uses an external magnetic field to separate magnetotactic bacteria according to their orientation in the magnetic field. At present, the magnetic separation methods of magnetotactic bacteria mainly include magnet adsorption, capillary magnetic separation and inverted magnetic separation. [0003] Magnetic adsorption and capillary magnetic separation have only micro-scale throughput. The inverted magnetic separation consists of a separation chamber and a sampling chamber, which are connected by a small hole. The device is placed upside down on the petri dish, a magnet is placed on the top, and it is left to stand for a period of time, and magnetotactic bacteria are obtained by sampling in the top sampling chamber. Because all of the magnetostatic blocks or si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42
CPCC12M47/04
Inventor 杨英杰邱冠周郭宁刘新星
Owner CENT SOUTH UNIV
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