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Culturing method of culture in bag and loading outside bag

A culture method and culture technology, applied in the fields of cell engineering and tissue engineering, can solve the problems of inconvenient disinfection of the loading head, increase the risk of culture contamination, etc., and achieve the effects of easy observation and mechanical performance testing, and low pollution

Inactive Publication Date: 2010-03-17
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this loading method, since the loading head is in direct contact with the culture, it is very inconvenient to implement the loading during the loading culture and to sterilize the loading head, which increases the risk of contamination of the culture.

Method used

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  • Culturing method of culture in bag and loading outside bag
  • Culturing method of culture in bag and loading outside bag
  • Culturing method of culture in bag and loading outside bag

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Embodiment Construction

[0021] Combine below Figure 1 to Figure 5 The culture method of bagging and loading out of the bag of the present invention will be described in detail.

[0022] first as figure 1 with figure 2 As shown, in order to prevent the culture from being polluted during the loading process and to facilitate observation and testing, the culture 1 is first packed in the packaging bag 3, and then the packaging bag 3 is put into the loading device in the cell culture box (not shown in the figure). (shown), the culture solution is perfused into the packaging bag 3 through the perfusion system, and a load is applied to the culture 1 outside the packaging bag 3 .

[0023] Wherein, the packaging bag 3 is a soft sealable bag or a part of hard packaging, and the packaging bag 3 is transparent, and can be used for culture observation and mechanical performance testing. The packaging jig 6 is arranged on the packaging bag 3, and the culture 1 After putting it into the packaging bag 3, tighte...

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PUM

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Abstract

The invention provides a culturing method of culture in bag and loading outside bag, comprising the following steps: placing the culture into a flexible and packaging transparent bag, leading in culture liquid into the packaging bag through a perfusion system, applying loading outside the bag to act on the culture and optimizing the growing environment of the culture. The culturing method has thecharacteristics of simple structure, low pollution and easy operation; and the packaging bag can be taken off at any time during the culturing process so as to carry out growing observation and mechanical property monitoring on the culture. The invention is convenient for clinical application and is beneficial to the production of large scale tissue-engineered products.

Description

technical field [0001] The invention relates to the field of cell engineering and tissue engineering, in particular to a culture method of bagging culture and loading outside the bag. Background technique [0002] Tissue loss or damage can cause serious health problems, and tissue engineering repair can overcome the disadvantages of current treatment methods and become an ideal bioremediation treatment. One of the main problems in the construction of artificial tissues is low mechanical properties and poor biochemical properties, and bioreactors play a key role in optimizing artificial tissues. [0003] In fact, many physical factors can affect the growth and development of cartilage tissue, and mechanical factors play a major role in it. Therefore, many researchers have developed a variety of bioreactors with mechanical environments to try to cultivate functional artificial tissues. The mechanical conditions of these reactors include: fluid shear stress, liquid pressure, t...

Claims

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Application Information

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IPC IPC(8): C12N5/00
Inventor 张春秋樊瑜波孙明林叶金铎李江徐艳
Owner TIANJIN UNIVERSITY OF TECHNOLOGY