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Method and assembly for optical reproduction with depth discrimination

An optical, deep technology, applied in the direction of using optical devices, optics, measurement devices, etc., can solve the problems of components and detectors that cannot match the lighting pattern, durable application barriers, etc.

Active Publication Date: 2013-07-10
迈克尔·施韦特纳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Probably the most important limitation is due to the fact that even small distortions in the optical image (e.g., barrel or pincushion distortion) result in an inability to match the elements of the illumination pattern to those of the detector at the subpixel level across the entire field of view.
Such a device would require highly corrected and well-tuned optics, which is an obstacle to widespread, robust applications

Method used

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  • Method and assembly for optical reproduction with depth discrimination
  • Method and assembly for optical reproduction with depth discrimination
  • Method and assembly for optical reproduction with depth discrimination

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Embodiment Construction

[0039] A method of producing an optical cross-sectional image by projecting two illumination patterns is described as follows:

[0040] For structured illumination, the illumination light projected onto the sample needs to be modulated in at least one spatial direction. Methods of structured illumination according to the state of the art require the projection of at least three illumination patterns or at least three phase positions of the periodic structure, respectively.

[0041] According to the invention, precisely two structures are projected, wherein it is advantageous if the intensity distribution of the second structure is complementary to that of the first structure and the average intensities of the two illumination patterns do not differ too much from each other.

[0042] Even though two-dimensional illumination patterns can also be used, for the sake of simplicity, grating structures will be used to explain the principles. Without limitation to the general case, i...

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Abstract

A method and an assembly for generating optical section images permit the three-dimensional, layered optical scanning of spatially extended objects. Illumination patterns with periodicity in at least one direction are projected into a plane and the light from the sample which is reflected and / or scattered and / or emitted fluorescence light is being imaged onto a spatially resolving detector. Initially, there is a calibration step, in which the local phase and / or the local period of the illumination patterns are determined for each location on the detector. In the sample detection mode, for the calculation of each optical section image there are two illumination patterns projected into or onto the sample and the resulting intensity distributions are used to form an image on the detector.

Description

technical field [0001] The invention relates to a method and a device for generating optical cross-sectional images. Background technique [0002] The present invention is suitable for three-dimensional, layer-by-layer scanning of spatially extended objects, and is mainly used in microscopy, but is not limited to this field. To generate a three-dimensional image or map of an object, the technique of optical sectioning is often used. A so-called optical section is an image that contains information of a certain range of depths. Accordingly, the optical system for generating the optical cross-sectional image performs selective imaging of these object details within the focal plane, whereas object details outside the focal plane are suppressed in the optical cross-sectional image. [0003] By recording a series of optical cross-sectional images at different focal positions, a three-dimensional object can be scanned step by step. Thus, a three-dimensional representation of an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01B11/24G02B21/00G02B21/16G06T7/00G01N21/64
CPCG01N21/6458G06T7/0065G02B21/367G01B11/2504G06T7/55
Inventor 迈克尔·施韦特纳
Owner 迈克尔·施韦特纳