Ralstonia solanacearum disease resistance high density identification method in field

A technology for tobacco bacterial wilt disease and an identification method, which is applied in the field of crop disease resistance identification, can solve the problems of difficulty in selecting disease-resistant and high-quality tobacco lines, fewer tobacco varieties, and low identification efficiency, thereby improving tobacco planting. The effect of density, high cost, and improved identification efficiency

Inactive Publication Date: 2010-04-07
INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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Problems solved by technology

If it is used as a variety breeding based on this, especially when the low-generation separation materials are used, the identification efficiency is low, and it is difficult

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  • Ralstonia solanacearum disease resistance high density identification method in field

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[0020] Below in conjunction with example the invention is further described in detail, but embodiment is not limitation of the present invention:

[0021] Since 2005, the inventor of this patent application has carried out experiments on tobacco breeding and plant protection in Yixing Village, Hanting Town, Xuancheng City, Anhui Province, and continuously cultivated tobacco greens in a 2hm paddy field every year. Blight-susceptible varieties, until the end of the test in 2007, the incidence of disease-susceptible varieties on about 1.2hm of land reached more than 80% at the end of growth. Since 2008, it was identified as a field with severe bacterial wilt, and began to use it as a disease nursery to carry out research on the identification of tobacco bacterial wilt resistance varieties and the selection of new varieties. From 2008 to 2009, the domestic tobacco bacterial wilt resistance resources and the screening research of local tobacco varieties in Anhui Province were carri...

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Abstract

The invention discloses a ralstonia solanacearum disease resistance high density identification method in the field, comprising: 1) cultivating ralstonia solanacearum disease nursery; 2) tidying disease nursery field in due time: before tobacco in the disease nursery is transplanted, tidying land to reach the requirement of ridging, furrowing and covering with a mulching film due to finely divided soil; according to the requirement that the width of each furrow is 1.5-2.5m and the ditch width between furrows is 0.5-0.7m, making a furrow, levelling furrow surface, and adjusting length according to the size of a plot; 3) determining cultivating density, and adopting a parallel furrow transplant mode: transplanting more than 3-5 lines in each furrow with the row spacing of 0.2-0.4m; 4) reasonably applying fertilizer, and strengthening field management: at the weight ratio of 1:1-2:1-2 of nitrogenous fertilizer, phosphatic fertilizer and potassic fertilizer, adopting a mulching film covering mode, and paying attention to field irrigation; and 5) inspecting and grading state of illness. The invention has the advantages that the ralstonia solanacearum disease resistance high density identification method in the field improves large field identification efficiency of ralstonia solanacearum disease resistance, can be applied to identify ralstonia solanacearum disease resistance, can improve identification material for above 4-6 times under the condition of same capital and disease nursery area and can be applied to breed ralstonia solanacearum disease.

Description

【Technical field】 [0001] The invention relates to a crop disease resistance identification method, in particular to an improvement of a field identification method for tobacco bacterial wilt resistance. 【Background technique】 [0002] Tobacco bacterial wilt is a bacterial disease caused by Ralstia solanacearum (Ralstonia solanacearum). Yield and quality are greatly affected, and it is a devastating disease on tobacco. The disease is widely distributed in tropical, subtropical and some warm regions of the world. It generally occurs in Fujian, Anhui, Jiangsu, Yunnan, Guangxi, Hebei, Hunan, Guangdong and other places in China. Utilizing disease-resistant varieties is a fundamental, effective and economical measure to control tobacco bacterial wilt. Since tobacco is a large crop compared with other field crops, the planting cost is higher, and limited by funding and other conditions, how to economically and quickly identify the resistance of tobacco to bacterial wilt has beco...

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Application Information

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IPC IPC(8): A01G1/00A01H1/04
Inventor 孙学永祖朝龙高正良周应兵阮龙杨华应
Owner INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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