In-situ remediation method using peat in underground water polluted by petroleum hydrocarbon as an additive medium
An in-situ remediation, petroleum hydrocarbon technology, applied in water/sludge/sewage treatment, chemical instruments and methods, biological water/sewage treatment, etc. and other problems, to achieve the effect of cheap price
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Embodiment 1
[0057] a. Suspend the soil sample of the polluted site with sterile water, stir it with magnetic force for 30 minutes at high speed, then filter the sundries and gravel with eight layers of gauze, let it rest for 1 hour, take 50mL of the suspension and transfer it to 200mL enrichment medium, and keep it at 10°C for low temperature , cultured at 120rpm for 15 days, then took an appropriate amount of bacterial suspension and reconnected it to the fresh enrichment medium, and adapted to the low temperature at 10°C; and then continuously subcultured for 5 times, each time for 9 days, gradually reducing the amount of auxiliary carbon source for each passage, until Petroleum hydrocarbons are the only carbon source;
[0058] b. Connect the above-mentioned native bacterial suspension to the inorganic salt medium with diesel as the only carbon source, continue to adapt and enrich at 10°C low temperature, and regularly monitor the growth of bacteria and the degradation of diesel componen...
Embodiment 2
[0067] a. Suspend the soil sample of the polluted site with sterile water, stir it with magnetic force for 30 minutes at high speed, then filter the sundries and gravel with eight layers of gauze, let it rest for 1 hour, take 50mL of the suspension and transfer it to 200mL enrichment medium, and keep it at 10°C for low temperature , cultured at 120rpm for 15 days, then took an appropriate amount of bacterial suspension and reconnected it to the fresh enrichment medium, and adapted to the low temperature at 10°C; and then continuously subcultured for 5 times, each time for 9 days, gradually reducing the amount of auxiliary carbon source for each passage, until Petroleum hydrocarbons are the only carbon source;
[0068] b. Connect the above-mentioned native bacterial suspension to the inorganic salt medium with diesel as the only carbon source, continue to adapt and enrich at 10°C low temperature, and regularly monitor the growth of bacteria and the degradation of diesel componen...
Embodiment 3
[0077] a. Suspend the soil sample of the polluted site with sterile water, stir it with magnetic force for 30 minutes at high speed, then filter the sundries and gravel with eight layers of gauze, let it rest for 1 hour, take 50mL of the suspension and transfer it to 200mL enrichment medium, and keep it at 10°C for low temperature , cultured at 120rpm for 15 days, then took an appropriate amount of bacterial suspension and reconnected it to the fresh enrichment medium, and adapted to the low temperature at 10°C; and then continuously subcultured for 5 times, each time for 9 days, gradually reducing the amount of auxiliary carbon source for each passage, until Petroleum hydrocarbons are the only carbon source;
[0078] b. Connect the above-mentioned native bacterial suspension to the inorganic salt medium with diesel as the only carbon source, continue to adapt and enrich at 10°C low temperature, and regularly monitor the growth of bacteria and the degradation of diesel componen...
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