In-situ remediation method using peat in underground water polluted by petroleum hydrocarbon as an additive medium

An in-situ remediation, petroleum hydrocarbon technology, applied in water/sludge/sewage treatment, chemical instruments and methods, biological water/sewage treatment, etc. and other problems, to achieve the effect of cheap price

Inactive Publication Date: 2010-04-28
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual restoration process, the PRB bioremediation efficiency may be low due to the low temperature in the groundwater environment, oligonutrients, and the possible competition among strains. At the same time, the aff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] a. Suspend the soil sample of the polluted site with sterile water, stir it with magnetic force for 30 minutes at high speed, then filter the sundries and gravel with eight layers of gauze, let it rest for 1 hour, take 50mL of the suspension and transfer it to 200mL enrichment medium, and keep it at 10°C for low temperature , cultured at 120rpm for 15 days, then took an appropriate amount of bacterial suspension and reconnected it to the fresh enrichment medium, and adapted to the low temperature at 10°C; and then continuously subcultured for 5 times, each time for 9 days, gradually reducing the amount of auxiliary carbon source for each passage, until Petroleum hydrocarbons are the only carbon source;

[0058] b. Connect the above-mentioned native bacterial suspension to the inorganic salt medium with diesel as the only carbon source, continue to adapt and enrich at 10°C low temperature, and regularly monitor the growth of bacteria and the degradation of diesel componen...

Embodiment 2

[0067] a. Suspend the soil sample of the polluted site with sterile water, stir it with magnetic force for 30 minutes at high speed, then filter the sundries and gravel with eight layers of gauze, let it rest for 1 hour, take 50mL of the suspension and transfer it to 200mL enrichment medium, and keep it at 10°C for low temperature , cultured at 120rpm for 15 days, then took an appropriate amount of bacterial suspension and reconnected it to the fresh enrichment medium, and adapted to the low temperature at 10°C; and then continuously subcultured for 5 times, each time for 9 days, gradually reducing the amount of auxiliary carbon source for each passage, until Petroleum hydrocarbons are the only carbon source;

[0068] b. Connect the above-mentioned native bacterial suspension to the inorganic salt medium with diesel as the only carbon source, continue to adapt and enrich at 10°C low temperature, and regularly monitor the growth of bacteria and the degradation of diesel componen...

Embodiment 3

[0077] a. Suspend the soil sample of the polluted site with sterile water, stir it with magnetic force for 30 minutes at high speed, then filter the sundries and gravel with eight layers of gauze, let it rest for 1 hour, take 50mL of the suspension and transfer it to 200mL enrichment medium, and keep it at 10°C for low temperature , cultured at 120rpm for 15 days, then took an appropriate amount of bacterial suspension and reconnected it to the fresh enrichment medium, and adapted to the low temperature at 10°C; and then continuously subcultured for 5 times, each time for 9 days, gradually reducing the amount of auxiliary carbon source for each passage, until Petroleum hydrocarbons are the only carbon source;

[0078] b. Connect the above-mentioned native bacterial suspension to the inorganic salt medium with diesel as the only carbon source, continue to adapt and enrich at 10°C low temperature, and regularly monitor the growth of bacteria and the degradation of diesel componen...

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PUM

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Abstract

The invention relates to an in-situ remediation method using peat in underground water polluted by petroleum hydrocarbon as an additive medium, which comprises: domesticating a strain; separating the strain; preprocessing peat; performing amplification culture of functional bacteria; immobilizing the functional bacteria receiving the amplification culture in the wet peat which is heated; uniformly mixing the peat carrying the functional bacteria and coarse sand in a certain volume ratio to prepare a reaction wall supporting medium; and filling the reaction wall supporting medium into a permeable reaction wall. The method has the advantages that: the peat used as a biological reaction wall additive medium can effectively immobilize functional bacteria, are insusceptible to flow away with underground water so as to be lost and can effectively absorb petroleum hydrocarbons, benzenes, toluenes, ethylbenzenes, dimethylbenzenes and naphthalines in the underground water, wherein the immobilization rate of the peat is 87 percent according to measurement in experiment and the absorption effect of the peat on polycyclic aromatic hydrocarbons is particularly obvious; nutrients are effectively fed to the functional bacteria in the reaction wall so as to ensure points in the reaction wall can keep high biomass in an operation period; and the peat used is cheap, widely and readily available and ecologically safe.

Description

Technical field: [0001] The invention relates to a groundwater-contaminated site restoration technology, in particular to a support medium and a permeable reaction wall for in-situ restoration of petroleum hydrocarbon-contaminated groundwater. Background technique: [0002] In view of the seriousness of petroleum hydrocarbons polluting groundwater, scholars from various countries have carried out extensive research on the control and restoration of petroleum hydrocarbons polluted groundwater. Among them, the permeable reactive barrier technology (Permeable Reactive Barrier, PRB) is a method emerging in many developed countries such as Europe and the United States for in-situ removal of polluted components in groundwater. [0003] The U.S. Environmental Protection Agency (USEPA) defines PRB as: placing active material walls underground to intercept pollution plumes, so that after the pollution plumes pass through the reaction medium, their pollutants can be converted into ano...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 张兰英杨悦锁马会强刘娜高松任何军
Owner JILIN UNIV
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