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Sperm cryopreservation method of Charybdisjaponica

A technology of cryopreservation and sperm, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of unsuitable sperm preservation in Japanese molluscs

Inactive Publication Date: 2010-05-12
HUAIHAI INST OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Japanese crab (Charybdis japonica A.Milne-Edwards) belongs to Arthropoda, Crustacea, Decapod, Portunidae, because the sperm of different kinds of animals has different biological characteristics, so the sperm preservation method disclosed in the prior art does not Suitable for the preservation of Japanese moth sperm

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  • Sperm cryopreservation method of Charybdisjaponica

Examples

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Embodiment 1

[0073] Example 1. A method for cryopreservation of the sperm of Japanese catfish, the steps are as follows:

[0074] (1) Material collection: From the middle of March to the end of August, take the male body of Japanese catfish at the mature stage of testis development, dissect it, take out the testis and put it in a mortar, add an appropriate amount of calcium-free artificial seawater to grind for 3-5 minutes, and then use 200 Remove tissue fragments by filtering with a mesh sieve, centrifuge at 1000 rpm for 5 minutes, obtain white sperm precipitate, dilute to 10 with diluent 6 Sperm suspension per ml, stored at 4°C for later use; the preparation method of the calcium-free artificial seawater is: NaCl 21.63g, NaOH 0.19g, KCl 1.12g, MgSO 4 ·7H 2O 4.93g, H 3 BO 3 0.53g, dissolved in 1L of distilled water; the preparation method of the diluent is: NaCl 25.0g, NaCl 2 SO 4 3.9g, KCl 0.73g, MgCl 2 ·6H 2 O 10.7g, NaHCO 3 0.23g, dissolved in 1L of distilled water, adjust th...

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Abstract

The invention discloses a sperm cryopreservation method of Charybdisjaponica, which is characterized by comprising the steps of material drawing, sperm activity identification, cooling balance, first-time precooling, second-time precooling, freezing and preserving and the like. Aiming at the biological characteristics of Charybdisjaponica sperm, the invention screens out a cryopreservation method suitable for Charybdisjaponica sperm, which can effectively preserve Charybdisjaponica sperm for a long term. After the Charybdisjaponica sperm is preserved in liquid nitrogen for one year by utilizing the method of the invention, the livability of unfrozen sperm can reach over 70%. The method has no need of expensive apparatus, simple operation, low cost and good effect, provides source guarantee of sperm for the artificial insemination of Charybdisjaponica, and can be used for other research fields such as hybridization breeding, germplasm preference, gynogenesis, species conservation and the like of Charybdisjaponica.

Description

technical field [0001] The invention relates to a method for preserving animal sperm, in particular to a method for freezing and preserving the sperm of Japanese catfish. Background technique [0002] In aquaculture production, with the development of artificial insemination technology and the need for germplasm resources protection, research on sperm in vitro preservation technology has attracted more and more attention. The long-term storage of sperm can ensure the stability of genes for a long time, and at the same time avoid the cost of raising parents, greatly improve the feasibility of breeding, and provide a technical platform for sperm metabolism and physiological research. [0003] Charybdis japonica has delicious meat, rich nutrition and moderate price, and the market demand is constantly increasing. Due to overfishing, the natural resources of Japanese crabs have decreased sharply, which is far from meeting the needs of human beings. Therefore, it is urgent to ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 许星鸿阎斌伦徐加涛徐国成浦寅芳许建和
Owner HUAIHAI INST OF TECH
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