Sugarcane tissue culture seedling bagging outdoor rooting culture method

Abstract

The invention provides a sugarcane tissue culture seedling bagging outdoor rooting culture method, which is characterized by comprising the following steps: putting a sugarcane liquid rooting culture medium into a plastic film bag, sterilizing at high temperature, inoculating, sealing, and putting into a greenhouse, wherein the rooting culture and the seedling hardening process are carried out at the same time under the outdoor natural conditions; and moving the tissue culture seedlings out of the bag after rooting, and planting in groups. Compared with the prior method, the method of the invention does not occupy the space of the culture room during rooting culture, has the advantages of low pollution rate, favorable light acceptance, good quality of roots and seedlings and the like, can remove the seedlings out of the bag for group planting for 5 days in advance, enhances the transplanting survival percent by more than 20%, is convenient for long-distance transportation, and is suitable for mass sugarcane tissue culture seedling rooting culture.

Description

technical field [0001] The invention relates to a method for tissue culture, in particular to a method for bagging outdoor rooting culture of sugarcane tissue culture seedlings. Background technique [0002] The application of tissue culture technology is the main technical means to speed up the breeding and popularization of new sugarcane varieties and the production of healthy seedlings free of viruses, and it has been applied on a large scale in production. At present, sugarcane tissue culture is the same as other crop tissues, and most of the culture containers are hard flasks, jars, and triangular flasks. Glass bottles are used in a large amount and are easy to wear and tear, especially because they take up a lot of space, resulting in a lot of waste of facilities and equipment, and high production costs; If the conditions are poor, the quality of the roots and seedlings is weak, and the survival rate of transplanting is low. To improve the quality of the roots and see...

AI Technical Summary

Problems solved by technology

Glass bottles are used in a large amount and are easy to wear and tear, especially because they take up a lot of space, resulting in a lot of waste of facilities and equipment, and high production costs; The conditions are poor, the quality of the roots and seedlings is weak, and the survival rate of transplanting is low. To improve the quality of the roots and seedlings, in addition to the need for a suitable medium, the most effective technical measure is to extend the time for rooting seedlings to harden outdoors

Adopting glass bottle to carry out rooting cultivation at present is not conducive to the hardening of tissue culture rooting seedlings except the above-mentioned shortcomings. The main manifestations are as follows. Large temperature changes cause the lid to expand with heat and contract with cold. There is an exchange of gas inside and outside the culture bottle, and air fungus enters the bottle, which greatly increases the pollution rate; Part of the nutrients and oxygen in the bottle have been absorbed and consumed during the rooting process of the tissue cultured seedlings. The nutritional conditions in the bottle are poor, and the hardening time should not be too long, otherwise the rooted seedlings will die due to insufficient nutrition. If the hardening time is insufficient, the hardening effect

[0003] In addition, other plants such as bananas are also produced in bags during the tissue culture process, but the medium is solid, and the production cost is relatively high