Method for structuring model for culturing genital tubercle organ for urethra ectogenesis

A technology for in vitro development and organ culture, applied in the fields of artificial cell constructs, biochemical equipment and methods, measurement/testing of microorganisms, etc., can solve the problems of complex mechanism, shortage, inconvenience, etc., and achieve a reasonable and easy-to-operate Effect

Inactive Publication Date: 2010-05-26
AFFILIATED HOSPITAL OF NANTONG UNIV
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Problems solved by technology

[0002] The pathogenesis of hypospadias is multifactorial and the mechanism is complicated. Genetic factors are one of the reasons for the occurrence of hypospadias. However, in recent years, the increase of endocrine disrupting chemicals in the environment, especially the increase of estrogen intake is considered to be the reason for the incidence of hypospadias. A key factor of the increase, we have successfully established an animal model of estrogen-in...

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  • Method for structuring model for culturing genital tubercle organ for urethra ectogenesis
  • Method for structuring model for culturing genital tubercle organ for urethra ectogenesis
  • Method for structuring model for culturing genital tubercle organ for urethra ectogenesis

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[0065] 1 material

[0066] Animal source: 8-10 weeks old C 57 BL / 6J inbred mice were provided by Shanghai Slack Experimental Animal Co., Ltd.

[0067] Main experimental materials:

[0068] Culture equipment: 30mm - CM hydrophilic PTFE membrane organotypic cell culture dish, 6-well cell culture plate.

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Abstract

The invention discloses a method for structuring a model for culturing genital tubercle organ for urethra ectogenesis, comprising the following steps of acquiring a mouse with accurate preggnancy, identifying embryo sex, cutting and culturing the genital tubercles, identifying the cultured genital tubercles, and the like. The invention has the advantages of reasonable route and easy operation andprovides an effective tool for further researching penile urethra development mechanism and genitalia malformation pathogenesis.

Description

Technical field: [0001] The invention relates to a method for constructing a urethral in vitro development model of genital tuberculosis organ culture. Background technique: [0002] The pathogenesis of hypospadias is multifactorial and the mechanism is complicated. Genetic factors are one of the reasons for the occurrence of hypospadias. However, in recent years, the increase of endocrine disrupting chemicals in the environment, especially the increase of estrogen intake is considered to be the reason for the incidence of hypospadias. A key factor of the increase, we have successfully established an animal model of estrogen-induced hypospadias to study the relationship between some endocrine disrupting chemicals and penile development, especially revealing the mechanism is crucial for the prevention and treatment of hypospadias , but there is currently a lack of relevant tools for the study of penis development. Animal model studies are effective but inconvenient. The devel...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12Q1/68A01K67/02A61B5/00G02B21/22
Inventor 马利民王忠张跃平周树军蔡波李文光吴优成兵沈维东农绍军陆凤英
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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