Human epididymis expression sperm binding protein HEL-178 and encoding gene and application thereof

Abstract

The invention belongs to biotechnology and medical field, and discloses a new human epididymis specific expression sperm binding protein HEL-178 and preparation and application thereof. The invention discloses an amino acid sequence of the HEL-178 protein and provides a recombination expression vector carrying a DNA sequence encoding the protein. The protein is positioned at the back region of the head of a mature sperm, an RT-PCR shows that higher micro-expression is mainly in epididymis, spermary, liver and spleen. A band of about 49 KD is detected the epididymis by using Western blot. The HEL-178 protein can be used as a target protein for the research and development of immunity acyeterion as well as clinical diagnosis and treatment of male infertility. The relevant gene or protein detecting method based on the protein HEL-178 can be widely applied to the reproductive medicine research field.

Description

technical field

[0001] The invention belongs to the field of biotechnology and medicine. Specifically, the present invention relates to a new human epididymis-expressed protein sperm-binding protein hereinafter referred to as HEL-178 and its coding gene and application Background technique

[0002] The development of human beings in this century is facing two serious problems: on the one hand, the global population is rapidly expanding and is expected to reach 9 billion in 2050; on the other hand, according to the World Health Organization survey, about 15% of couples are infertile The problem is that the incidence of infertility in developed countries has soared in the past 10 years, and it can be as high as 30% in developed countries in Europe, of which male factors account for about half. The World Health Organization predicts that with the increase of environmental pollution and sexually transmitted diseases and other pathogenic factors, infertility will become the thir...

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