Extract from fruit shell of camellia oleifera abel and preparation method and application thereof

An extract, Camellia oleifera technology, applied in the field of plant extracts, can solve the problems of limited application range, certain toxicity, unstable chemical structure, etc.

Inactive Publication Date: 2013-09-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cerulenin is the first FAS inhibitor discovered, but its chemical structure is unstable and has certain toxicity, which seriously limits its application range. Kuhajda et al. simulated the inhibition mechanism of cerulenin, synthesized and screened out Chemically stable FAS inhibitor C75

Method used

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  • Extract from fruit shell of camellia oleifera abel and preparation method and application thereof
  • Extract from fruit shell of camellia oleifera abel and preparation method and application thereof
  • Extract from fruit shell of camellia oleifera abel and preparation method and application thereof

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preparation example Construction

[0050] The preparation method of Camellia oleifera extract

[0051] Camellia oleifera extract provided by the invention can be prepared by the following method:

[0052] (1) Mix Camellia oleifera and alcohol-water solvent, and extract Camellia oleifera extract by one or more methods selected from the following: leaching, heat reflux extraction, countercurrent extraction, ultrasonic extraction, or microwave radiation extraction.

[0053] The Camellia oleifera raw material used in the present invention can be pretreated or not. The preferred raw material is 5-50 mesh chips with water content below 10w / w%.

[0054] The Camellia oleifera extract provided by the invention is obtained through alcohol-water solvent extraction. The alcohol-water solution is an alcohol solution with a volume percentage of 0-100%. Alcohols commonly used in this field can be used, such as ethanol and methanol, preferably ethanol. The extraction method is thermal reflux extraction, countercurrent extra...

Embodiment 1

[0099] Ultrasonic-assisted Extraction of Camellia oleifera Extract I

[0100] (1) Take Camellia oleifera, crush it and pass through a 50-mesh sieve, put it in a container, add 10 times the weight of water, and extract it by ultrasonic wave at 60°C for 20 minutes;

[0101] (2) Centrifuge the above-mentioned extract at 4000 rpm for 10 minutes, take the supernatant, and dilute it for use;

[0102] (3) Take the extract, dry it at 60°C, and calculate the yield of Camellia oleifera Extract I to be 11.9% (w / w).

[0103] Adopting aluminum nitrate-sodium nitrite colorimetric method to measure the total flavonoid content in every 100 grams of dry tea cattail is 3.05 grams, and the total flavonoid content in Camellia camellia extract I is 25.6% (calculated as rutin).

[0104] The yield of total phenols determined by Folin's phenol colorimetry was 2.31%, and the total phenol content in Camellia oleifera Extract I was 19.4% (calculated as gallic acid).

Embodiment 2

[0106] Ultrasonic-assisted Extraction of Camellia oleifera Extract II with 50% Ethanol

[0107] (1) Take Camellia oleifera, crush it and pass through a 50-mesh sieve, place it in a container, add 10 times the weight of 50% ethanol solution, and extract it by ultrasonic wave at 30°C for 30 minutes;

[0108] (2) The above extract was centrifuged at 4000 rpm for 10 minutes, the supernatant was taken, concentrated, and spray-dried to obtain Camellia oleifera Extract II.

[0109] The content of flavonoids in Camellia oleifera extract was determined by aluminum nitrate-sodium nitrite colorimetric method to be 28.5% (calculated as rutin).

[0110] The total phenol content in the extract of Camellia oleifera was determined to be 21.4% (calculated as gallic acid) by the Folin phenol colorimetric method.

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Abstract

The invention discloses an extract from the fruit shell of camellia oleifera abel and a preparation method and application thereof. Based on the total weight of the extract, the extract contains 5-70 w / weight percent of general flavone (in rutin); and camellia oleifera abel is the fruit shell of the woody plant camellia oleifera abel of the Camellia L category of the theaceae.

Description

technical field [0001] The present invention relates to the field of plant extracts. More specifically, it relates to an effective part extracted from the husk of camellia oleifera fruit, its preparation method and application. Background technique [0002] Camellia oleifera (Camellia oleifera Abel) is a woody plant of the genus Camellia in the family Theaceae. It is a unique oil tree species in my country. It is mainly distributed in Jiangxi, Hunan, Zhejiang, Guangxi, Guangdong, Fujian, Guizhou and other places in China. Camellia oleifera can be divided into white-flowered camellia, safflowered camellia and yellow-flowered camellia according to the color of the flowers. The most widely planted is white-flowered camellia, and ordinary camellia is the most widely distributed. Camellia oil is rich in unsaturated fatty acids and has high nutritional value. It is known as the olive oil of the East. At present, the planting area of ​​camellia oleifera in China is increasing yea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/82A61K8/97A61P35/00A61P39/06A61Q17/04A61K131/00
Inventor 沈建福陈秋平吴晓琴姜天甲王徐卿康海权
Owner ZHEJIANG UNIV
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