Periplocin composition, and preparation method thereof and application thereof in preparation of medicine for treating autoimmune diseases

A composition, the technology of barrella glucoside, applied in the field of autoimmune disease drugs, can solve the problems of difficult preparation, and the preparation method has not been reported in the literature, and achieve the effect of reducing costs

Active Publication Date: 2021-11-02
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

{References: Wang LY, Chen ZH, Zhou Y, et al. Structural revision of periplocosides and periperoxides, natural immunosuppressive agents from the genus Periploca. Phytochemistry, 2011, 72, 2230-2236.} The content is about 0.1%, and there are toxic components of cardiac glycosides and a large amount of fatty acids, low-grade oligosaccharides and 4-methoxy salicylaldehyde similar to the glycosides in the medicinal materials. The active ingredient (i.e. Salix polyglycoside) of salicyloside compounds is difficult to prepare, and its preparation method has not been reported in the literature

Method used

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  • Periplocin composition, and preparation method thereof and application thereof in preparation of medicine for treating autoimmune diseases
  • Periplocin composition, and preparation method thereof and application thereof in preparation of medicine for treating autoimmune diseases
  • Periplocin composition, and preparation method thereof and application thereof in preparation of medicine for treating autoimmune diseases

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preparation example Construction

[0085] According to another embodiment of the present disclosure, it provides a method for preparing a baracacid composition, comprising:

[0086] (1) Extraction and concentration steps

[0087] Add water to extract the root bark of Periploca sepium Bunge in Ascariaceae, discard the extract, extract the dregs with 40%-95% ethanol for 1-10 times, and combine the ethanol extracts Concentrate to obtain a suspension;

[0088] (2) Pre-column pretreatment step

[0089] Add an ethanol-water mixture to the suspension under stirring conditions so that the ethanol content is 10%-50%, and carry out solid-liquid separation; dissolve the solid in the ethanol-water mixture to make the ethanol content more than 60%, thereby obtaining a clear liquid, The clear liquid is extracted with a hydrocarbon solvent, and the raffinate is used as a sample liquid for a macroporous resin column;

[0090] Wherein, the hydrocarbon solvent is selected from a C5-C8 hydrocarbon solvent (such as cyclopentane...

Embodiment approach

[0098] According to another embodiment of the present disclosure, the preparation method includes

[0099] (1) Extraction and concentration steps

[0100] Take the root bark of Periploca sepium Bunge of Ascariaceae and add 2-10 times the mass of water to extract at 5-100°C for 2-10 hours, discard the extract, add 2 -10 times the mass of 40%-95% ethanol for 1-10 times, the ethanol extracts are combined and concentrated to 40% to 80% of the mass of the root bark of the raw material to obtain a suspension;

[0101] (2) Pre-column pretreatment step

[0102] Add 40%-95% ethanol to the suspension under stirring conditions to make the ethanol content 10%-50%. After stirring, the solid-liquid separation takes the precipitate, and then suspends the precipitate in 10%-50% ethanol again after stirring. Liquid separation to take the precipitate, then dissolve the precipitate in more than 60% ethanol, filter to obtain the supernatant, extract the supernatant with a hydrocarbon solvent fo...

preparation Embodiment 1

[0117] Preparation Example 1: Preparation of Salix polyglycoside (1)

[0118] Medicinal materials: 500g of Xiangjiapi medicinal materials.

[0119] Extraction: Discard the water extract after water extraction three times, then alcohol extract twice, adding 4L of 70% ethanol each time to obtain 8L alcohol extract.

[0120] Concentration: Concentrate the alcohol extract to obtain 300 g of suspension.

[0121]20% ethanol precipitation: add ethanol to the suspension to 20%, and separate the solid and liquid to obtain 56.175 g of 20% ethanol precipitation.

[0122] 35% ethanol precipitation: 35% ethanol precipitation was performed on the 20% ethanol precipitation, and 45.85 g of 35% ethanol precipitation was obtained by solid-liquid separation.

[0123] 70% ethanol precipitation: Add 2 times the volume of 95% ethanol and 4 times the volume of 70% ethanol to redissolve at 65°C, extract the supernatant to obtain 305 mL of 70% ethanol supernatant.

[0124] Cyclohexane extraction: 3...

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Abstract

The invention relates to a periplocin composition and a preparation method and application thereof. The periploside composition is prepared from the following components in parts by weight: 0.1-49 percent of Periploside A, 0.1-49 percent of Periploside C, 0.1-49 percent of Periploside D, 0.1-20 percent of Periploside E, 0.1-20 percent of Periploside K, 0.1-20 percent of Periploside O, 0.1-20 percent of Periploside Q and 0.1-20 percent of Periploside R. the pharmaceutical preparation taking periploca periploca polyglycoside disclosed by the invention as a pharmaceutical active component can protect the intestinal tissue structure and reduce inflammatory cell infiltration in the intestinal tissue; and the inflammation infiltration condition of joint tissues can be relieved, and the osteoerosion condition of arthritis can be relieved.

Description

technical field [0001] The present disclosure relates to a medicine for autoimmune diseases, and in particular, the present disclosure relates to a composition of baricacid, its preparation method and its use in the preparation of medicines for treating autoimmune diseases. Background technique [0002] Inflammatory bowel disease (Inflammatory Bowel Disease, IBD) is a collective term for a group of specific chronic, recurrent intestinal diseases, mainly including Crohn's disease (Crohn's disease, CD) and ulcerative colitis (Ulcerativecolitis, UC). kind. Ulcerative colitis is a chronic disease that causes inflammation and ulceration of the colon and rectum. The etiology has not been fully elucidated, and it may be related to genetic factors, autoimmune diseases, and changes in intestinal flora. The main symptoms during an attack include abdominal pain and diarrhea with bloody stools. Symptoms of weight loss, fever, and anemia may also occur during an ulcerative colitis attac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/704A61P37/06A61P1/00A61P29/00A61P19/02
CPCA61K31/7048A61K31/704A61P37/06A61P1/00A61P29/00A61P19/02A61K2300/00A61K31/58
Inventor 左建平赵维民林泽民陈振华何世君张如隽
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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