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Inula salsoloides extract and extraction method and usage thereof

An extraction method, the technology of Polygonum pombe, which is applied in the field of Polygonum pombe extract and its extraction, can solve the problems such as the extraction method and use of Polygonum pombe extract that have not yet been seen, and achieve the effect of inhibiting the activity of NO production

Inactive Publication Date: 2010-06-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Found through the document retrieval of prior art, do not see the report relevant with Polygonum officinale extract of the present invention and its extraction method, application as yet

Method used

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  • Inula salsoloides extract and extraction method and usage thereof
  • Inula salsoloides extract and extraction method and usage thereof
  • Inula salsoloides extract and extraction method and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of Polygonum sesquiterpene A

[0028] Polygonum officinalis 18.0kg, pulverized, extracted by percolation with 75% ethanol (volume fraction) 3 times, 20L each time, 24 hours;

[0029] The extract was concentrated under reduced pressure at 45°C to form an extract, and 1010.0 g of the extract was obtained, which was diluted with 2.5L of water, extracted three times with 8L petroleum ether, then extracted five times with 8L ethyl acetate, and recovered each extract under reduced pressure at 45°C. Some extracts with a relative density of 1.05 were obtained, and the pressure was controlled at 0.1 MPa.

[0030] The ethyl acetate part was subjected to silica gel (100-200 mesh) column chromatography, eluted with dichloromethane:methanol (100:1-1:1) gradient, followed by thin-layer chromatography, and was divided into 10 small parts. Part 5 was sequentially separated by silica gel (200-300 mesh) column chromatography and Sephadex LH-20 column chromatography, collecte...

Embodiment 2

[0032] Preparation of Inulalide B

[0033] Polygonum officinalis 18.0kg, pulverized, extracted by percolation with 75% ethanol (volume fraction) 3 times, 20L each time, 24 hours;

[0034] The extract was concentrated under reduced pressure at 45°C to form an extract, and 1010.0 g of the extract was obtained, which was diluted with 2.5L of water, extracted three times with 8L petroleum ether, then extracted five times with 8L ethyl acetate, and recovered each extract under reduced pressure at 45°C. Some extracts with a relative density of 1.05 were obtained, and the pressure was controlled at 0.1 MPa.

[0035] The ethyl acetate part was subjected to silica gel (100-200 mesh) column chromatography, eluted with dichloromethane:methanol (100:1-1:1) gradient, followed by thin-layer chromatography, and was divided into 10 small parts. Part 5 was sequentially separated by silica gel (200-300 mesh) column chromatography and Sephadex LH-20 column chromatography, collected dichlorometh...

Embodiment 3

[0037] Preparation of Polygonum sesquiterpene A

[0038] Polygonum officinalis 10.0kg, pulverized, extracted twice by percolation with 60% ethanol (volume fraction), 10L each time, 18 hours;

[0039] The extract was concentrated under reduced pressure at 50°C to form an extract, and 612g of extract was obtained. After diluting with 1L of water, it was first extracted 4 times with 5L petroleum ether, then extracted 5 times with 5L ethyl acetate, and the extracted parts were recovered under reduced pressure at 50°C to obtain For the extract with a relative density of 1.08, the pressure is controlled at 0.09Mpa;

[0040] The ethyl acetate part was subjected to silica gel (100-200 mesh) column chromatography, eluted with dichloromethane:methanol (100:1-1:1) gradient, followed by thin-layer chromatography, and was divided into 10 small parts. Part 5 was sequentially separated by silica gel (200-300 mesh) column chromatography and Sephadex LH-20 column chromatography, collected dic...

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Abstract

The invention relates to inula salsoloides extract and an extraction method and a usage thereof. Inula salsoloides sesquiterpenes A and inulasalsolide B are extracted from the inula salsoloides plant. The extraction method for the inula salsoloides extract comprises the following steps: step one, taking inula salsoloides, infiltrating ethyl alcohol into the inula salsoloides for extraction, and obtaining extracting solution; step two, concentrating the extracting solution, obtaining extractum, diluting the extractum by adding water, performing extraction by adopting petroleum ether and ethyl acetate in sequence, performing decompression to recover the extracted parts and obtaining extractum; step three, performing elution with silica gel column chromatography and obtaining inula salsoloides plant extract. The invention also relates to a usage of the inula salsoloides extract in the preparation of anti-inflammatory medicines. The inula salsoloides plant extract can well inhibit the activity generated by NO and has the anti-inflammatory function, thereby being used for preparing anti-inflammatory medicines.

Description

technical field [0001] The invention relates to an extract in the technical field of medicines and its extraction method and application, in particular to an extract of Polygonum chinensis and its extraction method and application. Background technique [0002] Inula salsoloides (Turc.) Ostenf. is a plant of the genus Inula in the family Asteraceae. The flowers of Polygonum chinensis and the whole plant before flowering can be used medicinally, and have the functions of antipyretic and diuretic, and the whole plant can also be used as the main medicine for treating vasculitis. It is widely distributed in Xinjiang, Inner Mongolia, northeastern Qinghai, Shaanxi, Hebei, Shanxi, and central and western Liaoning in China. Inula plants are widely distributed all over the world, and there are more than 100 species in the world, of which more than 25 species are distributed in China. Scholars at home and abroad have isolated flavonoids, sesquiterpene lactones, terpenoids and other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C35/205C07D307/93C07C29/76A61K31/047A61K31/365A61P29/00
Inventor 张卫东金慧子扈晓佳严诗楷
Owner SHANGHAI JIAO TONG UNIV
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