Application of pyrro-quinoline quinine in treating and preventing atherosclerosis
A technology for atherosclerosis and roloquinoline quinone, which is applied in the field of vascular diseases and can solve the problems of research on the prevention and/or treatment of atherosclerosis without PQQ active substances
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0089] Example 1: Inhibitory Effect of PQQ on Low Density Lipoprotein (LDL) Oxidation
[0090] experimental method:
[0091]1. Preparation of LDL: Take fresh plasma from healthy adults (obtained from volunteer donations), obtain an EDTA-containing LDL solution by density gradient sequence ultracentrifugation (reference 5: Lindgren, Jensen et al. 1969), and dissolve the solution in PBS The solution (pH7.5) was dialyzed and desalted (4°C, 4 hours), the resulting LDL solution was diluted to 200 mg / L, and the protein content was determined according to the Lowry method;
[0092] 2. LDL oxidation experiment: CuSO with a concentration of 5 μM 4 The LDL solution obtained in step 1 was diluted to a final concentration of 15 μg / ml to induce LDL oxidation (Reference 1: Esterbauer, Striegl et al., 1989). 25 μg / ml and 50 μg / ml of PQQ were added to the reaction system, and the LDL oxidation curve was drawn by measuring the absorbance of conjugated dienoic acid at 234 nm at different ti...
Embodiment 2
[0097] Example 2: The protective effect of PQQ on endothelial cells
[0098] experimental method:
[0099] 1. Cells and culture conditions: Under sterile conditions, the umbilical cords of newborn fetuses (obtained from Shanghai Obstetrics and Gynecology Hospital) were taken immediately after delivery from healthy puerperas, digested with 0.1% collagenase, and the obtained human umbilical vein endothelial cells were prepared according to Conventional adherent cell culture method for subculture (reference 4: Jaffe, Nachman et al. 1973);
[0100] The composition of the cell culture medium is: DMEM medium 1g, NaHCO 3 0.36g, 1000u each of penicillin and streptomycin, 10mL of fetal bovine serum, add water to 100mL. 37°C, 5% CO 2 Cultivate in an incubator, change the medium after 24 hours, passage and digest when the cell density is about 80%, and take the 2-3 generation cells for experiments;
[0101] 2. PQQ pretreatment: culture umbilical vein endothelial cells in a 96-well...
Embodiment 3
[0109] Example 3: The effect of PQQ on reducing the content of cholesterol in the arteries of atherosclerosis rat model
[0110] experimental method:
[0111] 1. Take several SD male rats aged 6 weeks and weighing about 220g, and raise them under standard conditions;
[0112] 2. The rats were randomly divided into 4 groups, 6 in each group. The groupings are as follows:
[0113] Group 1: Control group: ordinary SD rats fed with feed;
[0114] Group 2: High cholesterol diet group (HCD);
[0115] Group 3: High cholesterol plus low-dose PQQ group, PQQ was given with meals.
[0116] Group 4: High cholesterol plus high-dose PQQ group, PQQ was given with meals.
[0117] Wherein, the composition of high cholesterol diet in group 2-group 4 is: 2% cholesterol, 3% lard, 0.5% bile acid and 0.2% propylthiouracil, add in common feed;
[0118] The oral dose of PQQ in the low-dose PQQ group was: 0.5 mg / kg / d;
[0119] The oral dose of PQQ in the high-dose PQQ group was: 1.0 mg / kg / d. ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More