Extraction and separation method for sand starfish mucopolysaccharide

Abstract

The invention applies alkaline extraction method combined with enzymatic hydrolysis to extract acidic mucopolysaccharide from sand starfish, and sample powder has good solubility. The acidic mucopolysaccharide can be effectively discolored through hydrogen peroxide, and the binding proteins contained therein can be effectively removed through secondary enzymolysis process; and sand starfish acidic mucopolysaccharide I, II can be obtained after ethanol precipitation, wherein the total content of the mucopolysaccharides are up to 75% and 82% respectively, and protein contents thereof are 1.57% and 1.46% respectively. After further study on the mucopolysaccharide II, the mucopolysaccharide II further is found to be an acidic mucopolysaccharide containing sulfate and aldose residues without ketose and is composed of one major ingredient, the average molecular weight of the mucopolysaccharide II is about 12kD, and the charge distribution is uniform.

Description

Technical field: [0001] The invention relates to a method for extracting and separating the mucopolysaccharide of the starfish and its application, in particular to a method for separating the mucopolysaccharide of the starfish by using an alkali extraction method combined with an enzymatic hydrolysis method. Background technique: [0002] Acidic mucopolysaccharides, also known as Glycosaminoglycan, have various functions such as anti-virus, anti-tumor, anti-coagulation, lowering blood fat, anti-radiation and enhancing the immune function of the body, and have been widely valued and researched at present. , and has been used in medicine, cosmetics and other industries. Since the 1980s, domestic research on acidic mucopolysaccharides from animal sources has mainly focused on marine edible animals, and research on other animals has rarely been reported. Sand starfish belongs to Echinodermata (phylum Echinodermata) and class Asteroidea (Asteroidea). It is widely distributed in...

AI Technical Summary

Problems solved by technology

Sand starfish belongs to Echinodermata (phylum Echinodermata) and class Asteroidea (Asteroidea). It is distributed in large numbers along the coast of Shandong, especially in Jiaodong Peninsula.