Hepatitis A virus antibody assay kit and preparation method thereof

Abstract

The invention relates to a hepatitis A virus antibody assay kit which comprises an assay box, a gold labeled working solution bottle containing gold labeled working solution and a washing solution bottle containing washing solution, wherein the assay box comprises a box body and a box cover of the assay box, a cellulose nitrate membrane is arranged on the inner side of the box cover, a reaction hole is arranged on the box cover which corresponds to the position of the cellulose nitrate membrane, a water-absorbing pad is arranged in the box body of the assay box, an assay point and a quality control point are arranged on the cellulose nitrate membrane, the assay point is coated by solid-phase anti-human IgM, and the quality control point is coated by goat anti-mouse antibody. The hepatitis A virus antibody assay kit can fast diagnosis hepatitis A virus specific antibody by only dripping serum to be assayed on the cellulose nitrate membrane, and the hepatitis A virus antibody assay kit has the advantages of simple and convenient use, fast assay speed, no need of any instrument during clinical assay and simple structure and can avoid various factors during the experimental process from affecting the result and reduce the labor intensity of operation staff.

Description

technical field [0001] The invention relates to a hepatitis A virus antibody detection kit and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Hepatitis A virus, referred to as hepatitis A, is an intestinal infectious disease caused by hepatitis A virus (HAV). Hepatitis A virus (HAV) is a positive-strand RNA virus belonging to the Picornaviridae family Hepavirus. Hepatitis A is an infectious disease of the digestive tract, which can cause outbreaks and can lead to liver function damage in the early stage of infection. It is a common epidemic disease. It is generally transmitted through the fecal-oral route, which is highly contagious and widely spread, and can be transmitted on a large scale in a short period of time. Therefore, early accurate and specific diagnosis of hepatitis A is of great significance for the prevention and treatment of hepatitis A. [0003] At present, the laboratory diagnosis methods of HAV infect...

AI Technical Summary

Problems solved by technology

[0004] Metabolic inhibition test and enzyme-linked immunoassay are cumbersome to operate, requiring some larger instruments and equipment, complicated operation, expensive, and special personnel are required to operate the experiment, etc., which limit their application to a certain extent; complement fixation test has many components involved in the reaction , the influencing factors are complex, the operation steps are cumbersome and the requirements are very strict, and errors are prone to occur

Images