Hepatitis A virus antibody assay kit and preparation method thereof

A hepatitis A virus and antibody detection technology, which is applied in the direction of measuring devices, media-resistant diseases, instruments, etc., can solve the problems of cumbersome operation, error-prone, expensive, etc., and achieve simple and convenient use, reduce labor intensity, Detect fast effects

Inactive Publication Date: 2010-06-30
杨致亭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Metabolic inhibition test and enzyme-linked immunoassay are cumbersome to operate, requiring some larger instruments and equipment, complicated operation, expensive, and special personnel are required to operate the experiment, etc., which limit

Method used

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  • Hepatitis A virus antibody assay kit and preparation method thereof
  • Hepatitis A virus antibody assay kit and preparation method thereof
  • Hepatitis A virus antibody assay kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1, such as figure 1 As shown, the hepatitis A virus antibody detection kit includes a detection box, a gold standard working solution bottle 1 and a washing solution bottle 2 , and the detection box is placed in an aluminum foil bag 10 .

[0040] Such as figure 2 , image 3 As shown, the detection box includes a detection box body 6 and a box cover 3, the inside of the box cover 3 is provided with a nitrocellulose membrane 4, and the box cover 3 corresponding to the position of the nitrocellulose membrane 4 is provided with a reaction hole 9, and the detection box The box body 6 is equipped with a water-absorbing pad 5, and the nitrocellulose membrane 4 is equipped with a detection point 7 and a quality control point 8. The detection point 7 is coated with solid-phase anti-human IgM, and the quality control point 8 is coated with a goat anti-mouse antibody. .

[0041] The gold standard working solution bottle 1 contains the gold standard working solution, an...

Embodiment 2

[0077] Example 2, such as figure 1 As shown, the hepatitis A virus antibody detection kit includes a detection box, a gold standard working solution bottle 1 and a washing solution bottle 2 , and the detection box is placed in an aluminum foil bag 10 .

[0078] Such as figure 2 , image 3 As shown, the detection box includes a detection box body 6 and a box cover 3, the inside of the box cover 3 is provided with a nitrocellulose membrane 4, and the box cover 3 corresponding to the position of the nitrocellulose membrane 4 is provided with a reaction hole 9, and the detection box The box body 6 is equipped with a water-absorbing pad 5, and the nitrocellulose membrane 4 is equipped with a detection point 7 and a quality control point 8. The detection point 7 is coated with solid-phase anti-human IgM, and the quality control point 8 is coated with a goat anti-mouse antibody. .

[0079] The gold standard working solution bottle 1 contains the gold standard working solution, an...

Embodiment 3

[0115] Example 3, such as figure 1 As shown, the hepatitis A virus antibody detection kit includes a detection box, a gold standard working solution bottle 1 and a washing solution bottle 2 , and the detection box is placed in an aluminum foil bag 10 .

[0116] Such as figure 2 , image 3 As shown, the detection box includes a detection box body 6 and a box cover 3, the inside of the box cover 3 is provided with a nitrocellulose membrane 4, and the box cover 3 corresponding to the position of the nitrocellulose membrane 4 is provided with a reaction hole 9, and the detection box The box body 6 is equipped with a water-absorbing pad 5, and the nitrocellulose membrane 4 is equipped with a detection point 7 and a quality control point 8. The detection point 7 is coated with solid-phase anti-human IgM, and the quality control point 8 is coated with a goat anti-mouse antibody. .

[0117] The gold standard working solution bottle 1 contains the gold standard working solution, an...

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Abstract

The invention relates to a hepatitis A virus antibody assay kit which comprises an assay box, a gold labeled working solution bottle containing gold labeled working solution and a washing solution bottle containing washing solution, wherein the assay box comprises a box body and a box cover of the assay box, a cellulose nitrate membrane is arranged on the inner side of the box cover, a reaction hole is arranged on the box cover which corresponds to the position of the cellulose nitrate membrane, a water-absorbing pad is arranged in the box body of the assay box, an assay point and a quality control point are arranged on the cellulose nitrate membrane, the assay point is coated by solid-phase anti-human IgM, and the quality control point is coated by goat anti-mouse antibody. The hepatitis A virus antibody assay kit can fast diagnosis hepatitis A virus specific antibody by only dripping serum to be assayed on the cellulose nitrate membrane, and the hepatitis A virus antibody assay kit has the advantages of simple and convenient use, fast assay speed, no need of any instrument during clinical assay and simple structure and can avoid various factors during the experimental process from affecting the result and reduce the labor intensity of operation staff.

Description

technical field [0001] The invention relates to a hepatitis A virus antibody detection kit and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Hepatitis A virus, referred to as hepatitis A, is an intestinal infectious disease caused by hepatitis A virus (HAV). Hepatitis A virus (HAV) is a positive-strand RNA virus belonging to the Picornaviridae family Hepavirus. Hepatitis A is an infectious disease of the digestive tract, which can cause outbreaks and can lead to liver function damage in the early stage of infection. It is a common epidemic disease. It is generally transmitted through the fecal-oral route, which is highly contagious and widely spread, and can be transmitted on a large scale in a short period of time. Therefore, early accurate and specific diagnosis of hepatitis A is of great significance for the prevention and treatment of hepatitis A. [0003] At present, the laboratory diagnosis methods of HAV infect...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/558G01N33/544
CPCY02A50/30
Inventor 杨致亭管廷武单永德刘光福马金环
Owner 杨致亭
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