Method for improving growth trait of Fugu bimaculatus
A growth trait, the technology of redfin puffer, applied in the field of aquatic biological genetics and breeding, can solve the problems of high breeding cost, long breeding cycle, shortening the breeding cycle, etc., and achieve the effect of improving breeding efficiency, shortening the breeding cycle and reducing the breeding cost
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Embodiment 1
[0022] This embodiment takes the following steps:
[0023] (1) Select breeding broodstock
[0024] On April 15, 2008, 20 females of 3- to 4-year-old pufferfish and 10 males of pufferfish were selected.
[0025] (2) Maturity-promoting breeding and spawning of broodstock
[0026] Cultivate the broodstock of the two-spotted puffer and the red-finned puffer in separate ponds. The water temperature is controlled at 18-19°C. Calculated according to the volume of the water, 1 / 3-1 / 2 of the water is changed every day, and 8% of the body weight of the broodstock is fed to clamworms. Once, after 30 days of maturation-promoting cultivation, on May 15, inject a dose of 50 μg per kilogram of fish body weight to inject luteinizing hormone-releasing hormone analogue (LRH-A) to pufferfish; inject 30 μg per kilogram of fish body weight Dose injection of luteinizing hormone-releasing hormone analog (LRH-A) to redfin puffer;
[0027] (3) Artificial insemination and incubation
[0028] 72 hour...
Embodiment 3
[0046] (1) Select breeding broodstock
[0047] In April every year, 5-year-old puffer puffer females and puffer red-finned males are selected at a quantitative ratio of 2:1. The number depends on the number of seedlings. Generally, each female puffer puffer can produce 3 seedlings. Ten thousand tails or so.
[0048] (2) Maturity-promoting breeding and spawning of broodstock
[0049] Cultivate the broodstock of pufferfish and pufferfish in separate ponds, control the water temperature at 19°C, change 1 / 2 of the water every day, and feed clamworms once, the feeding amount is calculated as 8% of the body weight of the broodfish, and promote maturation after 40 days After the cultivation, the broodstock with mature selective glands was artificially induced, and a dose of 50 μg was injected per kilogram of fish body weight to inject luteinizing hormone-releasing hormone analogue (LRH-A) into pufferfish; a dose of 30 μg was injected per kilogram of fish body weight Injecting lutei...
Embodiment 4
[0058] (1) Select breeding broodstock
[0059] From March to April every year, 3 to 5-year-old female pufferfish and male pufferfish of the age of 3 to 5 are selected at a quantitative ratio of 2:1. The production of seedlings is about 30,000.
[0060] (2) Maturity-promoting breeding and spawning of broodstock
[0061] Two-spot puffer and red-fin puffer broodstock were cultivated in separate ponds, the water temperature was controlled at 18±1°C, calculated according to the volume of water, 1 / 3 to 1 / 2 of the water was changed every day, and clam worms were fed once, and the feeding amount was as follows: Calculate 8% of the body weight of the broodstock, after 30 to 40 days of maturation-promoting cultivation, the broodstock of selective gland development is artificially induced, and the dosage of 50 μg per kilogram of fish body weight is used to inject the luteinizing hormone releasing hormone analogue ( LRH-A); according to the dose of 30 μg injection per kilogram of fish b...
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