Check patentability & draft patents in minutes with Patsnap Eureka AI!

Secretory protein with reactionogenicity, coded nucleic acid and primer pair thereof

A technology for secreting proteins and encoding nucleic acids, which can be used in applications, analytical materials, plant genetic improvement, etc., and can solve problems that have not yet been seen.

Inactive Publication Date: 2010-08-11
SHANGHAI JIAO TONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching the literature of the prior art, it is found that there are no reports related to the reactogenic secreted protein of the present invention, its encoding nucleic acid and primer pairs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Secretory protein with reactionogenicity, coded nucleic acid and primer pair thereof
  • Secretory protein with reactionogenicity, coded nucleic acid and primer pair thereof
  • Secretory protein with reactionogenicity, coded nucleic acid and primer pair thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] The following examples will further illustrate the present invention in conjunction with the accompanying drawings. This embodiment is carried out on the premise of the technical solution of the present invention, and detailed implementation methods and processes are given, but the protection scope of the present invention is not limited to the following embodiments. For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed.

[0018] Step one, DNA extraction

[0019] M. bovis DNA extraction, Mycobacterium bovis (this bacterial strain has been published in "Liu Fang, Yan Yijia, Zhu Jianguo, Hua Xiuguo, Gao Qian; the expression of Mycobacterium bovis Mb1761c protein and its immunogenicity preliminary research [J] China Prevention Journal of Veterinary Medicine. 2008, 30 (11): 875-878 "disclosed) inoculated in Sutong (Sauton) medium, culture...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to secretory protein with reactionogenicity, coded nucleic acid and a primer pair thereof, which belongs to the field of biotechnology. The invention relates to protein which is coded by nucleic acid shown by a base sequence, such as SEQ ID NO.1; the invention discloses nucleic acid with a base sequence shown as SEQ ID NO.1; the invention also discloses a primer pair for amplifying the nucleic acid, an upstream primer has a base sequence shown as SEQ ID NO.2, a downstream primer has a base sequence shown as SEQ ID NO.3. The secretory protein with reactionogenicity can be used for detecting mycobacterium antibody in bovine serum and lays a good foundation for realizing high-flux bovine tuberculosis detection technique.

Description

technical field [0001] The invention relates to a protein in the field of biotechnology, its encoding nucleic acid and a primer pair, in particular to a reactogenic secreted protein, its encoding nucleic acid and a primer pair. Background technique [0002] Bovine tuberculosis is mainly a zoonotic infectious disease caused by Mycobacterium bovis (MTB), which seriously endangers human health and the development of animal husbandry and causes great economic losses. Mycobacterium bovis has a wide animal host spectrum, which also brings great difficulties to the control of the disease. Nowadays, the prevention and control of bovine tuberculosis mainly uses bovine tuberculin PPD for allergy diagnosis, and then isolates or kills the diagnosed positive cattle to achieve the purpose of eliminating the disease. However, the application of the control measures did not achieve the purpose of controlling the disease, but it has been on the rise in recent years. At present, the PPD ski...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/35C12N15/31C12N15/11G01N33/569
Inventor 朱建国刘芳林源
Owner SHANGHAI JIAO TONG UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com