Method for testing bioactivities of GLP-1 receptor agonist

A GLP-1, receptor agonist technology, applied in biochemical equipment and methods, microbial determination/inspection, color/spectral property measurement, etc., can solve the problems of high technical strength, long time, instability, etc
CN101798588AActive Publication Date: 2010-08-11SHANGHAI HUAYI BIO LAB CO LTD +1
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI HUAYI BIO LAB CO LTD
Publication Date
2010-08-11

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Abstract

The invention relates to a method for testing bioactivities, in particular to a method for testing the bioactivities of a GLP-1 receptor agonist. Based on the function of the GLP-1 receptor agonist which can promote mouse insulinoma cells (Min-6) to secrete the insulin, the method comprises the steps of: using insulin quantitatively secreted by a mouse / rat insulin kit to improve the precision of determination through a series of condition optimization processes, and testing the absorbency at the wavelength of 450 nm and the reference wavelength of 590 nm to obtain the effect curve that the GLP-1 receptor agonist induces the Min-6 cells to secrete the insulin; and finally testing the bioactivities of the GLP-1 receptor agonist on the basis of the effect curve.
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Description

technical field

[0001] The invention relates to a biological activity assay method, more specifically to a biological activity assay method of a GLP-1 receptor agonist. Background technique

[0002] Diabetes has become a major disease that threatens human life and health, and GLP-1 receptor agonists occupy an increasingly important position in diabetes drug research [Drug Review. 2009, 24(1): 32-33; Drug Review. 2008, 5(11):504-505]; GLP-1 receptor agonists mainly include glucagon-like peptide-1 (GLP-1), insulin-secreting peptide (Exendin-4), human GLP-1 analogues For Liraglutide, the determination of its biological activity has become a major difficulty because there is no international standard for activity determination [Chinese Journal of Biological Products. 2004, 17(1): 29-31].

[0003] The biological function of GLP-1 receptor agonists is mainly to specifically interact with the GLP-1 receptor of islet cells, causing changes in the corresponding signaling pathways an...

Claims

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