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Method for preparing bone tissue repairing material by using Arabic gum

A technology of gum arabic and repairing material, applied in the field of preparing bone tissue repairing materials, can solve the problems of low mechanical strength, fast degradation and high degree of swelling

Active Publication Date: 2013-04-24
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using collagen and hydroxyapatite as raw materials to prepare bone tissue repair materials by simulating bone tissue has become one of the hotspots in the field of biomaterials research, and has achieved certain research results: collagen / hydroxyapatite composite materials can be better It mimics the composition of bone and has good biocompatibility, osteoconductivity and osteoinductivity, but has disadvantages such as low mechanical strength, high swelling degree and fast degradation, which limit its clinical application.

Method used

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  • Method for preparing bone tissue repairing material by using Arabic gum
  • Method for preparing bone tissue repairing material by using Arabic gum
  • Method for preparing bone tissue repairing material by using Arabic gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for using gum arabic to prepare bone tissue repair materials, the steps are:

[0023] Take acetic acid aqueous solution with pH=3.0, dissolve collagen in it to prepare an acidic collagen aqueous solution with a concentration of 0.5% by weight; control the ambient temperature at 4°C, take 300mL of the prepared acidic collagen aqueous solution and perform the following operations:

[0024] 1) Add Na to the acidic collagen aqueous solution 2 HPO 4 Make its concentration reach 0.02mol / L, then add NaOH aqueous solution to adjust the pH value to 7.0 to obtain a near-neutral collagen solution;

[0025] 2) Mix the near-neutral collagen solution with 30 mL of gum arabic solution with a concentration of 5wt% (the weight ratio of collagen to gum arabic is 1:1), adjust the pH value to 7.0, stir for 20 minutes to make the system uniform, and obtain collagen / gum arabic mixture;

[0026] 3) Adjust the pH value of the collagen / gum arabic mixed solution to 7.2 with ammonia...

Embodiment 2

[0029] The method for using gum arabic to prepare bone tissue repair materials, the steps are:

[0030] Take acetic acid aqueous solution with pH=2.5, dissolve collagen in it to prepare 0.8wt% acidic collagen aqueous solution; control the ambient temperature at 6°C, take 187.5mL of prepared acidic collagen aqueous solution and perform the following operations:

[0031] 1) Add Na to the acidic collagen aqueous solution 2 HPO 4 Make its concentration reach 0.01mol / L, then add NaOH aqueous solution to adjust the pH value to 6.0 to obtain a near-neutral collagen solution;

[0032] 2) Mix the near-neutral collagen solution with 15 mL of gum arabic solution with a concentration of 5wt% (the weight ratio of collagen to gum arabic is 2:1), adjust the pH value to 6.0, stir for 18 minutes to make the system uniform, and then obtain collagen / gum arabic mixture;

[0033] 3) Adjust the pH value of the collagen / gum arabic mixed solution to 7.0 with NaOH aqueous solution, and then slowly ...

Embodiment 3

[0036] A method for preparing a bone tissue repair material by using gum arabic, the steps of which are as follows:

[0037]Take hydrochloric acid aqueous solution with pH=2.7, dissolve collagen in it to prepare 0.6wt% acidic collagen aqueous solution; control the ambient temperature at 10°C, take 412.5mL of prepared acidic collagen aqueous solution and perform the following operations:

[0038] 1) Add Na to the acidic collagen aqueous solution 2 HPO 4 Make its concentration reach 0.02mol / L, then add ammonia water to adjust the pH value to 6.6 to obtain a near-neutral collagen solution;

[0039] 2) Mix the near-neutral collagen solution with 16.5mL of 5wt% gum arabic solution (the weight ratio of collagen to gum arabic is 3:1), adjust the pH value to 6.6, stir for 10min to make the system uniform, and obtain collagen / arabic Gum mixed solution;

[0040] 3) Adjust the pH value of the collagen / gum arabic mixed solution to 8 with ammonia water, then slowly add 96 mL of 1.2 mol / ...

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Abstract

The invention relates to a method for preparing bone tissue repairing material by using Arabic gum, belonging to the field of the tissue engineering. The method comprises the following steps of: carrying out the following operations on prepared acidic collagen water solution at 0-10 DEG C: (1) adding phosphate into the acidic collagen water solution, and adding alkali to adjust the pH to 6-7 and obtain a nearly natural collagen water solution; (2) mixing the nearly natural collagen water solution and a water solution of the Arabic gum, adjusting the pH to 6-7, and uniformly stirring to obtaina collagen / Arabic gum mixing solution; (3) adding alkali to adjust the pH of the collagen / Arabic gum mixing solution to 7-8, slowly dropwise adding Ca(NO3) water solution and (NH4)2HPO4 water solution into the mixing solution, stirring for reacting for 1-4 h, and controlling the pH of the system to be 7-8 in the reaction process; aging and separating solid and liquid after the reaction is finished, washing the obtained solid repeatedly with distilled water to obtain the Col / HA / GumA composite material. The Col / HA / GumA composite material has the structural characteristics of natural bone, moderate supporting ability, good stability during in vitro enzymolysis and low cytotoxicity, is not easy to swell and is suitable to make bone tissue repairing material.

Description

technical field [0001] The invention belongs to the field of tissue engineering and relates to a method for using gum arabic to prepare bone tissue repair materials. Background technique [0002] In recent years, due to the aggravation of population aging and trauma, tumor, congenital deformity, infection, pathology and other factors, the number of patients with fractures and bone defects has increased significantly, and millions of patients with bone defects need surgical treatment every year. The traditional methods of treating bone defects include autologous bone grafting and allogeneic bone grafting. The former can be described as "treating injuries with injuries", which will lead to many complications and additional injuries; the latter is not only limited by donor sources and ethical issues, but also has Great risk of rejection and disease transmission. [0003] The concept of tissue engineering (Tissue Engineering) has provided a new idea and method for the repair of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20
Inventor 汤克勇冯文坡郑学晶
Owner ZHENGZHOU UNIV
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