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Method for feeding cotton bollworms in large scale

A large-scale technology for cotton bollworm, applied in animal husbandry and other directions, can solve the problems of limited research work, difficult tampon disinfection, and large operation workload, so as to reduce the incidence rate and reduce the spread of insect pathogens. simple effect

Inactive Publication Date: 2010-09-08
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

In addition, the disinfection of the cotton bollworm rearing utensils is very important for the artificial rearing of the cotton bollworm. The used glass tubes and glass utensils must be cleaned, dried and sterilized with cotton plugs in an oven at 120°C. Some of them are contaminated by mold. The tampon cannot be pulled out, and it needs to be sterilized by high-pressure moist heat, cleaned and dried, and then sterilized by dry heat in an oven; because the above-mentioned utensils are easily damaged, the tampon is not easy to be thoroughly sterilized, and the workload is large, the research work is difficult. Development is subject to certain restrictions

Method used

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Embodiment Construction

[0017] A method for raising cotton bollworms on a large scale, comprising the following steps in turn:

[0018] Step A: Disinfect and protect the egg surface: lay the eggs on the egg cloth, soak the egg cloth with a mixture of 1-5% formaldehyde and 2-15% HCL for 5 minutes, then rinse and dry with deionized water ;

[0019] Step B: After the eggs hatch, raise the larvae until pupation: Shake the hatched larvae directly from the egg cloth into a fresh-keeping box, cover them with plastic wrap and keep them moist for 3-5 days. Pick each head one by one from the fresh-keeping box into the worm-infestation board of cotton bollworm larvae, cover with plastic wrap and seal a circle around the larvae-infestation board with the plastic wrap to keep moisture, and raise them for about 10 days to pupate;

[0020] Step C: Disinfect and protect the pupal body: Remove the board to collect the cotton bollworm pupae 2 to 3 days after pupation, rinse with clean water first, and then immerse an...

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Abstract

The invention provides a method for feeding cotton bollworms in large scale, which sequentially comprises: a step A of sterilizing and protecting ovum surfaces; a step B of feeding larvae after ova until the larvae pupate; a step C of sterilizing and protecting pupal cells; and a step D of making the pupal cells changed into moths, paired and oviposit. The method has the advantages of reducing incidence rate in a process of artificially feeding the cotton bollworms in large scale, ensuring the quality, quantity and generational continuous healthy breeding of the cotton bollworms, and simultaneously realizing simple feeding operation and high breeding efficiency.

Description

technical field [0001] The invention relates to the field of biological artificial breeding, in particular to a method for raising cotton bollworms on a large scale. Background technique [0002] Establishing cotton bollworm strains, optimizing the artificial breeding technology of cotton bollworm, and providing a large number of healthy and well-developed test insects are the basic work of physiology, genetics, toxicology and other research. In the indoor large-scale breeding of cotton bollworm, in addition to providing suitable food and growth conditions, due to the killing characteristics of cotton bollworm larvae and the existence of entomopathogens in the environment, single-head feeding and strict disinfection of all links must be adopted during the breeding process to ensure breeding success. Thereby need to drop a large amount of manpower and material resources in the artificial breeding process of cotton bollworm, and the raising cost is higher. In the initial bre...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 何丽华牛宝龙翁宏飚顾卫平孟智启
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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